scholarly journals Unprecedented Treatment Strategy of Aquatic Environments: Oxidative Degradation of Penicillin G by Chromium Trioxide in Acidic Media and the Impact of Metal Ion Catalysts: Kinetics and Mechanistic Insights

ACS Omega ◽  
2020 ◽  
Vol 5 (50) ◽  
pp. 32781-32791
Author(s):  
Ahmed Fawzy ◽  
Arafat Toghan
Author(s):  
James W. E. Dickey ◽  
Neil E. Coughlan ◽  
Jaimie T. A. Dick ◽  
Vincent Médoc ◽  
Monica McCard ◽  
...  

AbstractThe influence of climate change on the ecological impacts of invasive alien species (IAS) remains understudied, with deoxygenation of aquatic environments often-overlooked as a consequence of climate change. Here, we therefore assessed how oxygen saturation affects the ecological impact of a predatory invasive fish, the Ponto-Caspian round goby (Neogobius melanostomus), relative to a co-occurring endangered European native analogue, the bullhead (Cottus gobio) experiencing decline in the presence of the IAS. In individual trials and mesocosms, we assessed the effect of high, medium and low (90%, 60% and 30%) oxygen saturation on: (1) functional responses (FRs) of the IAS and native, i.e. per capita feeding rates; (2) the impact on prey populations exerted; and (3) how combined impacts of both fishes change over invasion stages (Pre-invasion, Arrival, Replacement, Proliferation). Both species showed Type II potentially destabilising FRs, but at low oxygen saturation, the invader had a significantly higher feeding rate than the native. Relative Impact Potential, combining fish per capita effects and population abundances, revealed that low oxygen saturation exacerbates the high relative impact of the invader. The Relative Total Impact Potential (RTIP), modelling both consumer species’ impacts on prey populations in a system, was consistently higher at low oxygen saturation and especially high during invader Proliferation. In the mesocosm experiment, low oxygen lowered RTIP where both species were present, but again the IAS retained high relative impact during Replacement and Proliferation stages at low oxygen. We also found evidence of multiple predator effects, principally antagonism. We highlight the threat posed to native communities by IAS alongside climate-related stressors, but note that solutions may be available to remedy hypoxia and potentially mitigate impacts across invasion stages.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
A. Sukumaran ◽  
S. Pladwig ◽  
J. Geddes-McAlister

Abstract Background Microbial organisms encounter a variety of environmental conditions, including changes to metal ion availability. Metal ions play an important role in many biological processes for growth and survival. As such, microbes alter their cellular protein levels and secretion patterns in adaptation to a changing environment. This study focuses on Klebsiella pneumoniae, an opportunistic bacterium responsible for nosocomial infections. By using K. pneumoniae, we aim to determine how a nutrient-limited environment (e.g., zinc depletion) modulates the cellular proteome and secretome of the bacterium. By testing virulence in vitro, we provide novel insight into bacterial responses to limited environments in the presence of the host. Results Analysis of intra- and extracellular changes identified 2380 proteins from the total cellular proteome (cell pellet) and 246 secreted proteins (supernatant). Specifically, HutC, a repressor of the histidine utilization operon, showed significantly increased abundance under zinc-replete conditions, which coincided with an expected reduction in expression of genes within the hut operon from our validating qRT-PCR analysis. Additionally, we characterized a putative cation transport regulator, ChaB that showed significantly higher abundance under zinc-replete vs. -limited conditions, suggesting a role in metal ion homeostasis. Phenotypic analysis of a chaB deletion strain demonstrated a reduction in capsule production, zinc-dependent growth and ion utilization, and reduced virulence when compared to the wild-type strain. Conclusions This is first study to comprehensively profile the impact of zinc availability on the proteome and secretome of K. pneumoniae and uncover a novel connection between zinc transport and capsule production in the bacterial system.


2007 ◽  
Vol 53 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Jennifer R Huddleston ◽  
John C Zak ◽  
Randall M Jeter

Members of the bacterial genus Aeromonas are widely isolated from aquatic environments and studied in part for their ability to act as opportunistic pathogens in a variety of animals. All aeromonads, with the exception of Aeromonas trota, are generally thought to be resistant to ampicillin, so the antibiotic is frequently added to isolation medium as a selective agent. In this study, 282 aeromonads from environmental sources were isolated on a medium without ampicillin and their resistance to ampicillin determined. Of the 104 of these isolates that were judged to be independent (nonredundant), 18 (17.3%) were susceptible to ampicillin. A chi-square analysis was performed to determine the impact of ampicillin use on enumerating Aeromonas species from environmental samples. Our results indicate that, when ampicillin is used as a selective agent, a significant portion of the aeromonad population in at least some environ ments can be omitted from isolation.Key words: Aeromonas, ampicillin, selective media.


Author(s):  
Adnan Fareed ◽  
Abid Hussain ◽  
Mohsin Nawaz ◽  
Muhammad Imran ◽  
Zulfiqar Ali ◽  
...  

Author(s):  
Borut Kobal ◽  
Branko Cvjeticanin ◽  
Matija Barbič ◽  
Leon Meglič ◽  
Erik Škof ◽  
...  

Author(s):  
Diovana Napoleão ◽  
Letícia Alvarenga de Paula Eduardo ◽  
Roberta Veloso Garcia ◽  
Estaner Claro Romão

Environmental issues have been a prominent issue worldwide and the increase in plastic production and the lack of post-consumer waste management programs result in inadequate disposal and deposition in terrestrial and aquatic environments, causing environmental impacts. This paper was developed through the analysis of aspects of pedagogical practice in relation to environmental education with the plastic theme and its environmental impact on the environment. In this perspective, environmental education through the individual and the collectivity builds social values, knowledge, skills and competences aimed at the conservation of the environment. However, it was observed that addressing environmental issues should be considered a challenge for teachers, as it is a broad and diverse subject, as they are not prepared for the development of these activities and schools do not have the infrastructure to meet the needs of approaches to environmental issues.


2019 ◽  
Vol 2020 (6) ◽  
pp. 523-531 ◽  
Author(s):  
Yulia S. Kudyakova ◽  
Pavel A. Slepukhin ◽  
Marina S. Valova ◽  
Yanina V. Burgart ◽  
Viktor I. Saloutin ◽  
...  

2020 ◽  
Vol 14 (1) ◽  
pp. 234-241
Author(s):  
Reem Shammout ◽  
Raiean Al Habbal ◽  
Fadi Rayya

Iatrogenic porta hepatis injury is a rare but devastating surgical complication of laparoscopic cholecystectomy. There are no systematic studies examining the best treatment strategy in patients with this injury. We present a case of a 23-year-old woman with a large abscess in the right hepatic lobe due to an extreme vasculobiliary injury after laparoscopic cholecystectomy. Although rare, the impact of vasculobiliary injuries after cholecystectomy highlights the need for constant alertness and prompt management in order to minimize mortality and morbidity usually associated with the routine operative procedure.


Metallomics ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 925-935 ◽  
Author(s):  
Rohit Budhraja ◽  
Chang Ding ◽  
Philipp Walter ◽  
Stephan Wagner ◽  
Thorsten Reemtsma ◽  
...  

Absolute metal ion content was determined from whole cells of different microbial species and changes were related to growth conditions and change of encoded genes.


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