scholarly journals Transformation Cycle of Magnetosomes in Human Stem Cells: From Degradation to Biosynthesis of Magnetic Nanoparticles Anew

ACS Nano ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 1406-1417 ◽  
Author(s):  
Alberto Curcio ◽  
Aurore Van de Walle ◽  
Aida Serrano ◽  
Sandra Preveral ◽  
Christine Péchoux ◽  
...  
Keyword(s):  
Stem Cells ◽  
Magnetic Nanoparticles ◽  
Human Stem Cells ◽  
Transformation Cycle

scholarly journals Biosynthesis of magnetic nanoparticles from nano-degradation products revealed in human stem cells

2019 ◽  
Vol 116 (10) ◽  
pp. 4044-4053 ◽  
Author(s):  
Aurore Van de Walle ◽  
Anouchka Plan Sangnier ◽  
Ali Abou-Hassan ◽  
Alberto Curcio ◽  
Miryana Hémadi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Magnetic Nanoparticles ◽  
Degradation Products ◽  
Transformation Process ◽  
Human Cells ◽  
Human Stem Cells ◽  
Direct Demonstration ◽  
High Doses ◽  
Differentiation Status

While magnetic nanoparticles offer exciting possibilities for stem cell imaging or tissue bioengineering, their long-term intracellular fate remains to be fully documented. Besides, it appears that magnetic nanoparticles can occur naturally in human cells, but their origin and potentially endogenous synthesis still need further understanding. In an effort to explore the life cycle of magnetic nanoparticles, we investigated their transformations upon internalization in mesenchymal stem cells and as a function of the cells’ differentiation status (undifferentiated, or undergoing adipogenesis, osteogenesis, and chondrogenesis). Using magnetism as a fingerprint of the transformation process, we evidenced an important degradation of the nanoparticles during chondrogenesis. For the other pathways, stem cells were remarkably “remagnetized” after degradation of nanoparticles. This remagnetization phenomenon is the direct demonstration of a possible neosynthesis of magnetic nanoparticlesin celluloand could lay some foundation to understand the presence of magnetic crystals in human cells. The neosynthesis was shown to take place within the endosomes and to involve the H-subunit of ferritin. Moreover, it appeared to be the key process to avoid long-term cytotoxicity (impact on differentiation) related to high doses of magnetic nanoparticles within stem cells.

Testing Human Stem Cells in Primates

2005 ◽  
Vol 35 (16) ◽  
pp. 14
Author(s):  
GUY McKHANN
Keyword(s):  
Stem Cells ◽  
Human Stem Cells

Human stem cells transplantation to the subretinal space of the retina in the experiment

2020 ◽  
pp. 259-260
Author(s):  
A.A. Mikaelyan ◽  
◽  
N.L. Sheremet ◽  
A.Y. Andreev ◽  
A.A. Plyukhova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Subretinal Space ◽  
Human Stem Cells ◽  
Stem Cells Transplantation

Quantitative Evaluation of Chromosomal Rearrangements in Primary Gene-Edited Human Stem Cells by Preclinical CAST-Seq

2020 ◽  
Author(s):  
Giandomenico Turchiano ◽  
Geoffroy Andrieux ◽  
Georges Blattner ◽  
Valentina Pennucci ◽  
Julia Klermund ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantitative Evaluation ◽  
Chromosomal Rearrangements ◽  
Human Stem Cells ◽  
Primary Gene

Regeneration of pulp-dentin complex using human stem cells of the apical papilla: in vivo interaction with two bioactive materials

2021 ◽  
Author(s):  
Diana B. Sequeira ◽  
Ana Rafaela Oliveira ◽  
Catarina M. Seabra ◽  
Paulo J. Palma ◽  
Carlos Ramos ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Stem Cells ◽  
Bioactive Materials ◽  
Apical Papilla

scholarly journals Therapeutic Potential of Magnetic Nanoparticle-Based Human Adipose-Derived Stem Cells in a Mouse Model of Parkinson’s Disease

2021 ◽  
Vol 22 (2) ◽  
pp. 654
Author(s):  
Ka Young Kim ◽  
Keun-A Chang
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Magnetic Nanoparticles ◽  
Substantia Nigra ◽  
Mouse Model ◽  
Magnetic Nanoparticle ◽  
Imaging System ◽  
Therapeutic Potential ◽  
Adipose Derived Stem Cells

Parkinson’s disease (PD) is a progressive neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra. Several treatments for PD have focused on the management of physical symptoms using dopaminergic agents. However, these treatments induce various adverse effects, including hallucinations and cognitive impairment, owing to non-targeted brain delivery, while alleviating motor symptoms. Furthermore, these therapies are not considered ultimate cures owing to limited brain self-repair and regeneration abilities. In the present study, we aimed to investigate the therapeutic potential of human adipose-derived stem cells (hASCs) using magnetic nanoparticles in a 6-hydroxydopamine (6-OHDA)-induced PD mouse model. We used the Maestro imaging system and magnetic resonance imaging (MRI) for in vivo tracking after transplantation of magnetic nanoparticle-loaded hASCs to the PD mouse model. The Maestro imaging system revealed strong hASCs signals in the brains of PD model mice. In particular, MRI revealed hASCs distribution in the substantia nigra of hASCs-injected PD mice. Behavioral evaluations, including apomorphine-induced rotation and rotarod performance, were significantly recovered in hASCs-injected 6-OHDA induced PD mice when compared with saline-treated counterparts. Herein, we investigated whether hASCs transplantation using magnetic nanoparticles recovered motor functions through targeted brain distribution in a 6-OHDA induced PD mice. These results indicate that magnetic nanoparticle-based hASCs transplantation could be a potential therapeutic strategy in PD.

scholarly journals Telomere length set point regulation in human pluripotent stem cells critically depends on the shelterin protein TPP1

2020 ◽  
Vol 31 (23) ◽  
pp. 2583-2596
Author(s):  
John M. Boyle ◽  
Kelsey M. Hennick ◽  
Samuel G. Regalado ◽  
Jacob M. Vogan ◽  
Xiaozhu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Telomere Length ◽  
Pluripotent Stem Cells ◽  
Human Stem Cells ◽  
Set Point ◽  
Hypomorphic Allele ◽  
Point Control ◽  
Set Point Control ◽  
Stem Cell Lines

To better understand telomere length set point control in human stem cells, we generated knockout stem cell lines for TPP1 and contrasted their phenotypes with those of homozygous TPP1 L104A mutant stem cells. This comparison reveals that TPP1 L104A is not a hypomorphic allele but formally establishes TPP1 L104 as a dissociation of function mutant.

scholarly journals Cellular autophagy of human stem cells in the process of differentiation

2015 ◽  
Vol 26 (2) ◽  
pp. S21
Author(s):  
Shing-Hwa Lu ◽  
Hsin-Chen Lee ◽  
Alex T.L. Lin ◽  
Kuang-Kuo Chen ◽  
Luke S. Chang
Keyword(s):  
Stem Cells ◽  
Human Stem Cells ◽  
Cellular Autophagy
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