scholarly journals De Novo-Designed Near-Infrared Nanoaggregates for Super-Resolution Monitoring of Lysosomes in Cells, in Whole Organoids, and in Vivo

ACS Nano ◽  
2019 ◽  
Vol 13 (12) ◽  
pp. 14426-14436 ◽  
Author(s):  
Hongbao Fang ◽  
Shankun Yao ◽  
Qixin Chen ◽  
Chunyan Liu ◽  
Yuqi Cai ◽  
...  
Keyword(s):  
Near Infrared ◽  
De Novo ◽  
Super Resolution ◽  
In Cells

A new chloro-substituted dicyanoisophorone-based near-infrared fluorophore with a larger Stokes shift and its application for detecting cysteine in cells and in vivo

2020 ◽  
Vol 44 (36) ◽  
pp. 15432-15438
Author(s):  
Yu Wang ◽  
Wenda Zhang ◽  
Ting Ma ◽  
Duolu Li ◽  
Yubing Zhou ◽  
...  
Keyword(s):  
Near Infrared ◽  
Stokes Shift ◽  
Hydroxyl Group ◽  
Molecular Fluorescence ◽  
Fluorescence Bioimaging ◽  
In Cells

Many dicyanoisophorone-based fluorophores with an optical hydroxyl group have been explored to meet different imaging needs along with the rapid and wide development of molecular fluorescence bioimaging in recent years.

scholarly journals A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2556-2564 ◽  
Author(s):  
Runfeng Xu ◽  
Yue Wang ◽  
Huiyan You ◽  
Liangwei Zhang ◽  
Yunqing Wang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Reperfusion Injury ◽  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Real Time Monitoring ◽  
In Cells

A fluorescent probe, Cy-ArB, is developed for real-time monitoring of H2O2 fluctuations in cells and in vivo during ischemia/reperfusion processes.

Inhibitor structure-guided design and synthesis of near-infrared fluorescent probes for monoamine oxidase A (MAO-A) and its application in living cells and in vivo

2019 ◽  
Vol 55 (17) ◽  
pp. 2477-2480 ◽  
Author(s):  
Zhengmin Yang ◽  
Wenxiu Li ◽  
Hua Chen ◽  
Qingyuan Mo ◽  
Jun Li ◽  
...  
Keyword(s):  
Monoamine Oxidase ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Monoamine Oxidase A ◽  
Specific Detection ◽  
Design And Synthesis ◽  
Mao A ◽  
In Cells

A series of near-infrared fluorescent probes based on inhibitor (clorgyline) structure-guided design were synthesized for the specific detection of MAO-A in cells and in vivo.

scholarly journals Correction: A ratiometric fluorescent probe for peroxynitrite prepared by de novo synthesis and its application in assessing the mitochondrial oxidative stress status in cells and in vivo

2018 ◽  
Vol 54 (93) ◽  
pp. 13159-13159 ◽  
Author(s):  
Dong-Ye Zhou ◽  
Yongfei Li ◽  
Wen-Li Jiang ◽  
Yang Tian ◽  
Junjie Fei ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fluorescent Probe ◽  
De Novo ◽  
De Novo Synthesis ◽  
Mitochondrial Oxidative Stress ◽  
Oxidative Stress Status ◽  
Ratiometric Fluorescent Probe ◽  
In Cells

Correction for ‘A ratiometric fluorescent probe for peroxynitrite prepared by de novo synthesis and its application in assessing the mitochondrial oxidative stress status in cells and in vivo’ by Dong-Ye Zhou et al., Chem. Commun., 2018, 54, 11590–11593.

scholarly journals Phosphorothioate Antisense Oligonucleotides Induce the Formation of Nuclear Bodies

1998 ◽  
Vol 9 (5) ◽  
pp. 1007-1023 ◽  
Author(s):  
Peter Lorenz ◽  
Brenda F. Baker ◽  
C. Frank Bennett ◽  
David L. Spector
Keyword(s):  
Antisense Oligonucleotides ◽  
De Novo ◽  
Regulation Of Gene Expression ◽  
Nuclear Body ◽  
Nuclear Bodies ◽  
Phosphorothioate Oligodeoxynucleotides ◽  
Nuclear Structures ◽  
In Cells

Antisense oligonucleotides are powerful tools for the in vivo regulation of gene expression. We have characterized the intracellular distribution of fluorescently tagged phosphorothioate oligodeoxynucleotides (PS-ONs) at high resolution under conditions in which PS-ONs have the potential to display antisense activity. Under these conditions PS-ONs predominantly localized to the cell nucleus where they accumulated in 20–30 bright spherical foci designated phosphorothioate bodies (PS bodies), which were set against a diffuse nucleoplasmic population excluding nucleoli. PS bodies are nuclear structures that formed in cells after PS-ON delivery by transfection agents or microinjection but were observed irrespectively of antisense activity or sequence. Ultrastructurally, PS bodies corresponded to electron-dense structures of 150–300 nm diameter and resembled nuclear bodies that were found with lower frequency in cells lacking PS-ONs. The environment of a living cell was required for the de novo formation of PS bodies, which occurred within minutes after the introduction of PS-ONs. PS bodies were stable entities that underwent noticeable reorganization only during mitosis. Upon exit from mitosis, PS bodies were assembled de novo from diffuse PS-ON pools in the daughter nuclei. In situ fractionation demonstrated an association of PS-ONs with the nuclear matrix. Taken together, our data provide evidence for the formation of a nuclear body in cells after introduction of phosphorothioate oligodeoxynucleotides.

scholarly journals Guiding biomolecular interactions in cells using de novo protein-protein interfaces

2018 ◽  
Author(s):  
Abigail J Smith ◽  
Franziska Thomas ◽  
Deborah Shoemark ◽  
Derek N Woolfson ◽  
Nigel J Savery
Keyword(s):  
Rational Design ◽  
De Novo ◽  
Gene Activation ◽  
Low Complexity ◽  
Coiled Coils ◽  
Biomolecular Interactions ◽  
Biomolecular Systems ◽  
Binding Domains ◽  
In Cells

An improved ability to direct and control biomolecular interactions in living cells would impact on synthetic biology. A key issue is the need to introduce interacting components that act orthogonally to endogenous proteomes and interactomes. Here we show that low-complexity, de novo designed protein-protein-interaction (PPI) domains can substitute for natural PPIs and guide engineered protein-DNA interactions in Escherichia coli. Specifically, we use de novo homo- and hetero-dimeric coiled coils to reconstitute a cytoplasmic split adenylate cyclase; to recruit RNA polymerase to a promoter and activate gene expression; and to oligomerize both natural and designed DNA-binding domains to repress transcription. Moreover, the stabilities of the heterodimeric coiled coils can be modulated by rational design and, thus, adjust the levels of gene activation and repression in vivo. These experiments demonstrate the possibilities for using designed proteins and interactions to control biomolecular systems such as enzyme cascades and circuits in cells.

A high-performance enzyme-activated near-infrared probe for the sensing and tracking of tumor-related NQO1 in cells and in vivo

2022 ◽  
Vol 354 ◽  
pp. 131129
Author(s):  
Yong Zhang ◽  
Xueqian Chen ◽  
Qing Yuan ◽  
Yongning Bian ◽  
Mingrui Li ◽  
...  
Keyword(s):  
High Performance ◽  
Near Infrared ◽  
In Cells
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