scholarly journals A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2556-2564 ◽  
Author(s):  
Runfeng Xu ◽  
Yue Wang ◽  
Huiyan You ◽  
Liangwei Zhang ◽  
Yunqing Wang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Reperfusion Injury ◽  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Real Time Monitoring ◽  
In Cells

A fluorescent probe, Cy-ArB, is developed for real-time monitoring of H2O2 fluctuations in cells and in vivo during ischemia/reperfusion processes.

scholarly journals Intravital imaging of real-time endogenous actin dysregulation in proximal and distal tubules at the onset of severe ischemia-reperfusion injury

2021 ◽  
Author(s):  
Peter R. Corridon ◽  
Shurooq H. Karam ◽  
Ali A. Khraibi ◽  
Anousha A. Khan ◽  
Mohamed A. Alhashmi
Keyword(s):  
Reperfusion Injury ◽  
Real Time ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Renal Ischemia ◽  
Two Photon Microscopy ◽  
Real Time Visualization ◽  
Distal Tubules ◽  
Actin Expression

Abstract Severe renal ischemia-reperfusion injury (IRI) can lead to acute and chronic kidney dysfunction. Cytoskeletal modifications are among the main effects of this condition. The majority of studies that have contributed to the current understanding of IRI have relied on histological analyses using exogenous probes after the fact. Here we report the successful real-time visualization of actin cytoskeletal alterations in live proximal and distal tubules that arise at the onset of severe IRI. To achieve this, we induced fluorescent actin expression in these segments in rats with hydrodynamic gene delivery (HGD). Using intravital two-photon microscopy we then tracked and quantified endogenous actin dysregulation that occurred by subjecting these animals to 60 minutes of bilateral renal ischemia. Rapid (by 1-hour post-reperfusion) and significant (up to 50%) declines in actin content were observed. The decline in fluorescence within proximal tubules was significantly greater than that observed in distal tubules. Actin-based fluorescence was not recovered during the measurement period extending 24 hours post-reperfusion. Such injury decimated the renal architecture, in particular, actin brush borders, and hampered the reabsorptive and filtrative capacities of these tubular compartments. Thus, for the first time, we show that the combination of HGD and intravital microscopy can serve as an experimental tool to better understand how IRI modifies the cytoskeleton in vivo and provide an extension to current histopathological techniques.

A tumor-targeted near-infrared fluorescent probe for HNO and its application to the real-time monitoring of HNO release in vivo

2021 ◽  
Author(s):  
Ziyin Chai ◽  
Diankai Liu ◽  
Xiaoyi Li ◽  
Yanyan Zhao ◽  
Wen Shi ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Real Time Monitoring ◽  
The Real

A tumor-targeted near-infrared fluorescent probe has been developed for monitoring the intratumoral release of HNO from its donors in vivo.

scholarly journals Intravital imaging of real-time endogenous actin dysregulation in proximal and distal tubules at the onset of severe ischemia-reperfusion injury

2021 ◽  
Author(s):  
Peter R Corridon ◽  
Shurooq H Karam ◽  
Ali A Khraibi ◽  
Anousha A Khan ◽  
Mohamed A Alhashmi
Keyword(s):  
Reperfusion Injury ◽  
Real Time ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Renal Ischemia ◽  
Two Photon Microscopy ◽  
Real Time Visualization ◽  
Distal Tubules ◽  
Actin Expression

Severe renal ischemia-reperfusion injury (IRI) can lead to acute and chronic kidney dysfunction. Cytoskeletal modifications are among the main effects of this condition. The majority of studies that have contributed to the current understanding of IRI have relied on histological analyses using exogenous probes after the fact. Here we report the successful real-time visualization of actin cytoskeletal alterations in live proximal and distal tubules that arise at the onset of severe IRI. To achieve this, we induced fluorescent actin expression in these segments in rats with hydrodynamic gene delivery (HGD). Using intravital two-photon microscopy we then tracked and quantified endogenous actin dysregulation that occurred by subjecting these animals to 60 minutes of bilateral renal ischemia. Rapid (by 1-hour post-reperfusion) and significant (up to 50%) declines in actin content were observed. The decline in fluorescence within proximal tubules was significantly greater than that observed in distal tubules. Actin-based fluorescence was not recovered during the measurement period extending 24 hours post-reperfusion. Such injury decimated the renal architecture, in particular, actin brush borders, and hampered the reabsorptive and filtrative capacities of these tubular compartments. Thus, for the first time, we show that the combination of HGD and intravital microscopy can serve as an experimental tool to better understand how IRI modifies the cytoskeleton in vivo and provide an extension to current histopathological techniques.

Real-time monitoring of γ-Glutamyltranspeptidase in living cells and in vivo by near-infrared fluorescent probe with large Stokes shift

Talanta ◽  
2019 ◽  
Vol 191 ◽  
pp. 126-132 ◽  
Author(s):  
Feiyan Liu ◽  
Zhen Wang ◽  
Tianyu Zhu ◽  
Wenli Wang ◽  
Biao Nie ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Stokes Shift ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Large Stokes Shift
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