Core–Shell Distinct Nanodrug Showing On-Demand Sequential Drug Release To Act on Multiple Cell Types for Synergistic Anticancer Therapy

ACS Nano ◽  
2019 ◽  
Vol 13 (6) ◽  
pp. 7036-7049 ◽  
Author(s):  
Jinsheng Huang ◽  
Yongmin Xu ◽  
Hong Xiao ◽  
Zecong Xiao ◽  
Yu Guo ◽  
...  
Keyword(s):  
Drug Release ◽  
Cell Types ◽  
Anticancer Therapy ◽  
Core Shell ◽  
On Demand ◽  
Multiple Cell ◽  
Sequential Drug Release

Layer-by-layer printing of cells and its application to tissue engineering

2004 ◽  
Vol 845 ◽  
Author(s):  
Priya Kesari ◽  
Tao Xu ◽  
Thomas Boland
Keyword(s):  
Tissue Engineering ◽  
Microelectromechanical Systems ◽  
Cell Types ◽  
Three Dimensions ◽  
Layer By Layer ◽  
Hematopoietic Stem ◽  
On Demand ◽  
Cell Structures ◽  
Aqueous Environments ◽  
Multiple Cell

ABSTRACTTissues and organs exhibit distinct shapes and functions nurtured by vascular connectivity. In order to mimic and examine these intricate structure-function relationships, it is necessary to develop efficient strategies for assembling tissue-like constructs. Many of the top-down fabrication techniques used to build microelectromechanical systems, including photolithography, are attractive due to the similar feature sizes, but are not suitable for delicate biological systems or aqueous environments. A layer-by layer approach has been proposed by us to pattern functional cell structures in three dimensions. Freeform cell structures are created by the inkjet method, in which cells are entrapped within hydrogels and crosslinked on demand. The cells are viable, functional and show potential for cell maturation as exemplified by the diversion of hematopoietic stem cells into multiple cell types. These results show promise for many tissue engineering applications.

Polymer-controlled core–shell nanoparticles: a novel strategy for sequential drug release

RSC Advances ◽  
2014 ◽  
Vol 4 (57) ◽  
pp. 30430-30439 ◽  
Author(s):  
Yang Cao ◽  
Bochu Wang ◽  
Yazhou Wang ◽  
Deshuai Lou
Keyword(s):  
Drug Release ◽  
Plga Nanoparticles ◽  
Therapeutic Agents ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Miscible Liquids ◽  
Novel Strategy ◽  
Core Shell Nanoparticles ◽  
Sequential Drug Release ◽  
Coaxial Electrospray

Immiscible and miscible liquids were utilized to fabricate PVP/PLGA and PCL/PLGA nanoparticles with a distinct core–shell structure by coaxial electrospray. Two different sequential drug release profiles from different nanoparticles were observed. The melanoma cells and endothelial cells can be sequentially targeted and killed by therapeutic agents released from nanoparticles.

Bioinspired peptosomes with programmed stimuli-responses for sequential drug release and high-performance anticancer therapy

Nanoscale ◽  
2017 ◽  
Vol 9 (27) ◽  
pp. 9317-9324 ◽  
Author(s):  
Yuan Li ◽  
Wei Li ◽  
Weier Bao ◽  
Bin Liu ◽  
Dan Li ◽  
...  
Keyword(s):  
Drug Release ◽  
High Performance ◽  
Anticancer Therapy ◽  
Anticancer Efficacy ◽  
Sequential Drug Release

Bioinspired peptosomes based on natural α-lactalbumin were developed with triple-responses for sequential drug release and high anticancer efficacy.

Core–Shell Chitosan Microcapsules for Programmed Sequential Drug Release

2016 ◽  
Vol 8 (16) ◽  
pp. 10524-10534 ◽  
Author(s):  
Xiu-Lan Yang ◽  
Xiao-Jie Ju ◽  
Xiao-Ting Mu ◽  
Wei Wang ◽  
Rui Xie ◽  
...  
Keyword(s):  
Drug Release ◽  
Core Shell ◽  
Sequential Drug Release

scholarly journals Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms

2021 ◽  
Vol 3 (2) ◽  
pp. 166-181 ◽  
Author(s):  
Alexandra A. C. Newman ◽  
Vlad Serbulea ◽  
Richard A. Baylis ◽  
Laura S. Shankman ◽  
Xenia Bradley ◽  
...  
Keyword(s):  
Atherosclerotic Lesion ◽  
Cell Types ◽  
Fibrous Cap ◽  
Multiple Cell

scholarly journals MyD88 Is Not Required for Muscle Injury-Induced Endochondral Heterotopic Ossification in a Mouse Model of Fibrodysplasia Ossificans Progressiva

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 630
Author(s):  
Huili Lyu ◽  
Cody M. Elkins ◽  
Jessica L. Pierce ◽  
C. Henrique Serezani ◽  
Daniel S. Perrien
Keyword(s):  
Heterotopic Ossification ◽  
Muscle Injury ◽  
Cell Types ◽  
Fibrodysplasia Ossificans Progressiva ◽  
Inflammatory Signaling ◽  
Conditional Deletion ◽  
Pathway Crosstalk ◽  
Multiple Cell

Excess inflammation and canonical BMP receptor (BMPR) signaling are coinciding hallmarks of the early stages of injury-induced endochondral heterotopic ossification (EHO), especially in the rare genetic disease fibrodysplasia ossificans progressiva (FOP). Multiple inflammatory signaling pathways can synergistically enhance BMP-induced Smad1/5/8 activity in multiple cell types, suggesting the importance of pathway crosstalk in EHO and FOP. Toll-like receptors (TLRs) and IL-1 receptors mediate many of the earliest injury-induced inflammatory signals largely via MyD88-dependent pathways. Thus, the hypothesis that MyD88-dependent signaling is required for EHO was tested in vitro and in vivo using global or Pdgfrα-conditional deletion of MyD88 in FOP mice. As expected, IL-1β or LPS synergistically increased Activin A (ActA)-induced phosphorylation of Smad 1/5 in fibroadipoprogenitors (FAPs) expressing Alk2R206H. However, conditional deletion of MyD88 in Pdgfrα-positive cells of FOP mice did not significantly alter the amount of muscle injury-induced EHO. Even more surprisingly, injury-induced EHO was not significantly affected by global deletion of MyD88. These studies demonstrate that MyD88-dependent signaling is dispensable for injury-induced EHO in FOP mice.

scholarly journals Microtubule–microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes

2016 ◽  
Vol 113 (34) ◽  
pp. E4995-E5004 ◽  
Author(s):  
Wen Lu ◽  
Michael Winding ◽  
Margot Lakonishok ◽  
Jill Wildonger ◽  
Vladimir I. Gelfand
Keyword(s):  
Cytoplasmic Streaming ◽  
Cell Types ◽  
Nurse Cells ◽  
Wild Type ◽  
Actin Cortex ◽  
Microtubule Motor ◽  
Multiple Cell ◽  
Cytoplasmic Microtubules ◽  
Two Populations

Cytoplasmic streaming in Drosophila oocytes is a microtubule-based bulk cytoplasmic movement. Streaming efficiently circulates and localizes mRNAs and proteins deposited by the nurse cells across the oocyte. This movement is driven by kinesin-1, a major microtubule motor. Recently, we have shown that kinesin-1 heavy chain (KHC) can transport one microtubule on another microtubule, thus driving microtubule–microtubule sliding in multiple cell types. To study the role of microtubule sliding in oocyte cytoplasmic streaming, we used a Khc mutant that is deficient in microtubule sliding but able to transport a majority of cargoes. We demonstrated that streaming is reduced by genomic replacement of wild-type Khc with this sliding-deficient mutant. Streaming can be fully rescued by wild-type KHC and partially rescued by a chimeric motor that cannot move organelles but is active in microtubule sliding. Consistent with these data, we identified two populations of microtubules in fast-streaming oocytes: a network of stable microtubules anchored to the actin cortex and free cytoplasmic microtubules that moved in the ooplasm. We further demonstrated that the reduced streaming in sliding-deficient oocytes resulted in posterior determination defects. Together, we propose that kinesin-1 slides free cytoplasmic microtubules against cortically immobilized microtubules, generating forces that contribute to cytoplasmic streaming and are essential for the refinement of posterior determinants.

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