Super-resolution Imaging of Individual Human Subchromosomal Regions in Situ Reveals Nanoscopic Building Blocks of Higher-Order Structure

ACS Nano ◽  
2018 ◽  
Vol 12 (5) ◽  
pp. 4909-4918 ◽  
Author(s):  
Ke Fang ◽  
Xuecheng Chen ◽  
Xiaowei Li ◽  
Yi Shen ◽  
Jielin Sun ◽  
...  
Keyword(s):  
Super Resolution ◽  
Building Blocks ◽  
Higher Order ◽  
Order Structure ◽  
Resolution Imaging ◽  
Individual Human

scholarly journals In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT

2017 ◽  
pp. 231-252 ◽  
Author(s):  
Brian J. Beliveau ◽  
Alistair N. Boettiger ◽  
Guy Nir ◽  
Bogdan Bintu ◽  
Peng Yin ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Yanxiang Ni ◽  
Bo Cao ◽  
Tszshan Ma ◽  
Gang Niu ◽  
Yingdong Huo ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Molecular Beacon ◽  
Genomic Sequence ◽  
Single Cells ◽  
Nuclear Genome ◽  
Super Resolution ◽  
Resolution Limit ◽  
Fish Method ◽  
Resolution Imaging

High-resolution visualization of short non-repetitive DNA in situ in the nuclear genome is essential for studying looping interactions and chromatin organization in single cells. Recent advances in fluorescence in situ hybridization (FISH) using Oligopaint probes have enabled super-resolution imaging of genomic domains with a resolution limit of 4.9 kb. To target shorter elements, we developed a simple FISH method that uses molecular beacon (MB) probes to facilitate the probe-target binding, while minimizing non-specific fluorescence. We used three-dimensional stochastic optical reconstruction microscopy (3D-STORM) with optimized imaging conditions to efficiently distinguish sparsely distributed Alexa-647 from background cellular autofluorescence. Utilizing 3D-STORM and only 29–34 individual MB probes, we observed 3D fine-scale nanostructures of 2.5 kb integrated or endogenous unique DNA in situ in human or mouse genome, respectively. We demonstrated our MB-based FISH method was capable of visualizing the so far shortest non-repetitive genomic sequence in 3D at super-resolution.

scholarly journals Super-Resolution Imaging of Higher-Order Chromatin Structures at Different Epigenomic States in Single Mammalian Cells

Cell Reports ◽  
2018 ◽  
Vol 24 (4) ◽  
pp. 873-882 ◽  
Author(s):  
Jianquan Xu ◽  
Hongqiang Ma ◽  
Jingyi Jin ◽  
Shikhar Uttam ◽  
Rao Fu ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Super Resolution ◽  
Higher Order ◽  
Resolution Imaging

scholarly journals Spin-Orbit Angular Momentum Conversion in Metamaterials and Metasurfaces

2019 ◽  
Vol 1 (1) ◽  
pp. 91-106 ◽  
Author(s):  
Graciana Puentes
Keyword(s):  
Angular Momentum ◽  
Quantum Communication ◽  
Super Resolution ◽  
Building Blocks ◽  
New Physics ◽  
Spin Orbit ◽  
Optical Fields ◽  
Precise Control ◽  
Resolution Imaging ◽  
And Performance

In the last decades, unprecedented progress in the manipulation of the spin angular momentum (SAM) and orbital angular momentum (OAM) of light has been achieved, enabling a number of applications, ranging from classical and quantum communication to optical microscopy and super-resolution imaging. Metasurfaces are artificially engineered 2D metamaterials with designed subwavelength-size building blocks, which allow the precise control of optical fields with unparalleled flexibility and performance. The reduced dimensionality of optical metasurfaces enables new physics and leads to functionalities and applications that are remarkably different from those achievable with bulk materials. In this review, we present an overview of the progress in optical metasurfaces for the manipultation of SAM and OAM of light, for applications in integrated spin-orbit conversion (SOC) devices.

Photonic Nanojet Sub-Diffraction Nano-Fabrication With in situ Super-Resolution Imaging

2019 ◽  
Vol 18 ◽  
pp. 226-233 ◽  
Author(s):  
Yangdong Wen ◽  
Haibo Yu ◽  
Wenxiu Zhao ◽  
Feifei Wang ◽  
Xiaoduo Wang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Nano Fabrication ◽  
Photonic Nanojet ◽  
Resolution Imaging

scholarly journals Super-Resolution Fluorescence Microscopy of Protein Association and Higher-Order Structure

2018 ◽  
Vol 114 (3) ◽  
pp. 348a-349a
Author(s):  
Adriano Vissa ◽  
Maximiliano Giuliani ◽  
William S. Trimble ◽  
Peter K. Kim ◽  
Christopher M. Yip
Keyword(s):  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Higher Order ◽  
Order Structure ◽  
Protein Association

Single-wavelength-controlled in situ dynamic super-resolution fluorescence imaging for block copolymer nanostructures via blue-light-switchable FRAP

2016 ◽  
Vol 15 (11) ◽  
pp. 1433-1441 ◽  
Author(s):  
Wen-Liang Gong ◽  
Jie Yan ◽  
Ling-Xi Zhao ◽  
Chong Li ◽  
Zhen-Li Huang ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Block Copolymers ◽  
Fluorescence Imaging ◽  
Blue Light ◽  
Super Resolution ◽  
Resolution Imaging

A blue-light-switchable fluorophore enables single-wavelength controlledin situdynamic super-resolution imaging of block copolymers.

Transitions between in situ and isolated chromatin

1993 ◽  
Vol 105 (2) ◽  
pp. 551-561 ◽  
Author(s):  
P.J. Giannasca ◽  
R.A. Horowitz ◽  
C.L. Woodcock
Keyword(s):  
Higher Order ◽  
Chromatin Fiber ◽  
Sperm Chromatin ◽  
Order Structure ◽  
Native State ◽  
Chromatin Fibers

We show that the mechanism by which chromatin displaying higher-order structure is usually isolated from nuclei involves a transition to an extended nucleosomal arrangement. After being released from nuclei, chromatin must refold in order to produce the typical chromatin fibers observed in solution. For starfish sperm chromatin with a long nucleosome repeat (222 bp), isolated fibers are significantly wider than those in the nucleus, indicating that the refolding process does not regenerate the native higher-order structure. We also propose that for typical eukaryotic nuclei, the concept that the native state of the (inactive) bulk of the genome is a chromatin fiber with defined architecture be reconsidered.

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