Three-Dimensional Orientation of Anisotropic Plasmonic Aggregates at Intracellular Nuclear Indentation Sites by Integrated Light Sheet Super-Resolution Microscopy

ACS Nano ◽  
2018 ◽  
Vol 12 (5) ◽  
pp. 4156-4163 ◽  
Author(s):  
Suresh Kumar Chakkarapani ◽  
Yucheng Sun ◽  
Seungah Lee ◽  
Ning Fang ◽  
Seong Ho Kang
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Light Sheet ◽  
Super Resolution Microscopy

Cubic spline-based depth-dependent localization of mitochondria–endoplasmic reticulum contacts by three-dimensional light-sheet super-resolution microscopy

The Analyst ◽  
2021 ◽  
Author(s):  
Yucheng Sun ◽  
Seungah Lee ◽  
Seong Ho Kang
Keyword(s):  
Endoplasmic Reticulum ◽  
Calcium Homeostasis ◽  
Three Dimensional ◽  
Super Resolution ◽  
Light Sheet ◽  
Cubic Spline ◽  
Contact Distance ◽  
Super Resolution Microscopy

The contact distance between mitochondria (Mito) and endoplasmic reticulum (ER) has received considerable attention owing to their crucial function in maintaining lipid and calcium homeostasis. Herein, cubic spline algorithm-based depth-dependent...

A Multi-Functional Microfluidic Device Compatible with Widefield and Light Sheet Imaging

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Regan P Moore ◽  
Ellen C O’Shaughnessy ◽  
Yu Shi ◽  
Ana T Nogueira ◽  
Katelyn M Heath ◽  
...  
Keyword(s):  
High Resolution ◽  
Microfluidic Device ◽  
Super Resolution ◽  
Light Sheet ◽  
Ethylene Propylene ◽  
Super Resolution Microscopy ◽  
Fluorinated Ethylene Propylene

We present a microfluidic device compatible with high resolution light sheet and super-resolution microscopy. Our device is a 150 μm thick chamber with a transparent fluorinated ethylene propylene (FEP) cover...

Combination Light-Sheet Illumination with Super-Resolution Three-Dimensional Fluorescence Microimaging

2018 ◽  
Vol 45 (3) ◽  
pp. 0307006 ◽  
Author(s):  
谢新林 Xie Xinlin ◽  
陈蓉 Chen Rong ◽  
赵宇轩 Zhao Yuxuan ◽  
费鹏 Fei Peng
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Light Sheet

scholarly journals Small-Molecule Labeling of Live Cell Surfaces for Three-Dimensional Super-Resolution Microscopy

2014 ◽  
Vol 136 (40) ◽  
pp. 14003-14006 ◽  
Author(s):  
Marissa K. Lee ◽  
Prabin Rai ◽  
Jarrod Williams ◽  
Robert J. Twieg ◽  
W. E. Moerner
Keyword(s):  
Small Molecule ◽  
Three Dimensional ◽  
Super Resolution ◽  
Live Cell ◽  
Cell Surfaces ◽  
Super Resolution Microscopy

scholarly journals GPU-accelerated real-time reconstruction in Python of three-dimensional datasets from structured illumination microscopy with hexagonal patterns

2021 ◽  
Vol 379 (2199) ◽  
Author(s):  
Hai Gong ◽  
Wenjun Guo ◽  
Mark A. A. Neil
Keyword(s):  
Moving Objects ◽  
Three Dimensional ◽  
Simulated Data ◽  
Super Resolution ◽  
Light Sheet ◽  
Processing Unit ◽  
Structured Illumination ◽  
Reconstruction Algorithms ◽  
Structured Illumination Microscopy ◽  
Axially Moving

We present a structured illumination microscopy system that projects a hexagonal pattern by the interference among three coherent beams, suitable for implementation in a light-sheet geometry. Seven images acquired as the illumination pattern is shifted laterally can be processed to produce a super-resolved image that surpasses the diffraction-limited resolution by a factor of over 2 in an exemplar light-sheet arrangement. Three methods of processing data are discussed depending on whether the raw images are available in groups of seven, individually in a stream or as a larger batch representing a three-dimensional stack. We show that imaging axially moving samples can introduce artefacts, visible as fine structures in the processed images. However, these artefacts are easily removed by a filtering operation carried out as part of the batch processing algorithm for three-dimensional stacks. The reconstruction algorithms implemented in Python include specific optimizations for calculation on a graphics processing unit and we demonstrate its operation on experimental data of static objects and on simulated data of moving objects. We show that the software can process over 239 input raw frames per second at 512 × 512 pixels, generating over 34 super-resolved frames per second at 1024 × 1024 pixels. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.

scholarly journals A Review on Dual-Lens Fluorescence Microscopy for Three-Dimensional Imaging

2020 ◽  
Vol 8 ◽  
Author(s):  
Xiaoyan Li ◽  
Yubing Han ◽  
Wenjie Liu ◽  
Cuifang Kuang ◽  
Xu Liu ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
3D Imaging ◽  
Signal To Noise Ratio ◽  
Three Dimensional ◽  
Super Resolution ◽  
Light Sheet ◽  
Animal Cells ◽  
3D Images ◽  
Single Lens ◽  
Model Animal

Three-dimensional (3D) imaging using dual-lens fluorescence microscopies is popular in observing fluorescently labeled biological samples, such as mammalian/model animal cells, tissues, and embryos. Specifically, dual-lens super-resolution fluorescence microscopy methods using two opposing objective lenses allow significantly higher axial resolution and better signal to noise ratio than traditional single-lens counterparts, and thus distinguish more details in 3D images of fine intracellular structures. For 3D imaging of thick tissues and entire embryos, dual-lens light-sheet fluorescence microscopy methods using two objective lenses, either orthogonal or non-orthogonal, to achieve selective plane illumination, can meet the requirements, and thus can be used to observe embryo development and structures of interest in thick tissues. This review summarizes both dual-lens fluorescence microscopy methods, including their principles, configurations, and 3D imaging applications, providing a guideline for biological laboratories with different 3D imaging needs.

Sign in / Sign up

Export Citation Format

Share Document