Accurate Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluorescence Correlation Spectroscopy

ACS Nano ◽  
2015 ◽  
Vol 9 (7) ◽  
pp. 7360-7373 ◽  
Author(s):  
Karel Goossens ◽  
Mira Prior ◽  
Victor Pacheco ◽  
Dieter Willbold ◽  
Klaus Müllen ◽  
...  
Keyword(s):  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Organic Media ◽  
Fluorescence Correlation

Monte Carlo simulation of fluorescence correlation spectroscopy data

2011 ◽  
Vol 76 (3) ◽  
pp. 207-222 ◽  
Author(s):  
Peter Košovan ◽  
Filip Uhlík ◽  
Jitka Kuldová ◽  
Miroslav Štěpánek ◽  
Zuzana Limpouchová ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Rotational Diffusion ◽  
Correlation Spectroscopy ◽  
Analytical Models ◽  
Spherical Particles ◽  
Fluorescence Signal ◽  
Fluorescence Correlation

We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones. It means that the current data treatment yields apparent values of diffusion coefficients and other parameters only and the interpretation of experimental results for systems of particles with sizes comparable to the size of the active illuminated volume requires great care and precaution.

How To Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy

Langmuir ◽  
2003 ◽  
Vol 19 (10) ◽  
pp. 4120-4126 ◽  
Author(s):  
A. Benda ◽  
M. Beneš ◽  
V. Mareček ◽  
A. Lhotský ◽  
W. Th. Hermens ◽  
...  
Keyword(s):  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Confocal Fluorescence
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