Real-Time Study of Protein Phase Separation with Spatiotemporal Analysis of Single-Nanoparticle Trajectories

ACS Nano ◽  
2020 ◽  
Author(s):  
Qi Pan ◽  
Daxiao Sun ◽  
Jianfeng Xue ◽  
Jie Hao ◽  
Hansen Zhao ◽  
...  
1999 ◽  
Vol 597 ◽  
Author(s):  
L. V. Natarajan ◽  
R. L. Sutherland ◽  
R. T. Pogue ◽  
V. P. Tondiglia ◽  
D. Tomlin ◽  
...  

AbstractThe formation of holographic reflection gratings in polymer dispersed liquid crystals(H-PDLC) was investigated in real time both in reflection and transmission modes. Real time study is useful for obtaining information on the speed of grating formation, phase separation dynamics, host polymer shrinkage, polarization dependence of the reading beam, bleaching of the photointiator dye and scattering of the writing beam during grating formation. The real time studies showed that the formation of the grating is fast and reaches a maximum in a few seconds. The diffraction efficiency (DE) VS time curve shows an initial increase followed by a sharp decrease and a recovery to higher efficiencies. Variation of monomer functionality in the pre-polymer syrup resulted in a decrease of reflection efficiency as the functionality is lowered. Varying the writing power of the laser beam gave higher efficiencies at powers exceeding 10 mW/cm2. Liquid crystal (LC) loadings of >16% are needed to obtain measurable efficiencies. Varying the polarization of the probe beam (S vs P) revealed differences in the temporal evolution of DE. Morphology studies are indicative of the differences in the phase separation due to monomer functionality, LC concentration and also laser power. The growth dynamics of H-PDLC gratings are very different from the well known Du Pont photopolymer films which may be due to the phase separation process accompanying gelation in H-PDLC systems.


Author(s):  
Jenni Myllykoski ◽  
Anniina Rantakari

This chapter focuses on temporality in managerial strategy making. It adopts an ‘in-time’ view to examine strategy making as the fluidity of the present experience and draws on a longitudinal, real-time study in a small Finnish software company. It shows five manifestations of ‘in-time’ processuality in strategy making, and identifies a temporality paradox that arises from the engagement of managers with two contradictory times: constructed linear ‘over time’ and experienced, becoming ‘in time’. These findings lead to the re-evaluation of the nature of intention in strategy making, and the authors elaborate the constitutive relation between time as ‘the passage of nature’ and human agency. Consequently, they argue that temporality should not be treated merely as an objective background or a subjective managerial orientation, but as a fundamental characteristic of processuality that defines the dynamics of strategy making.


Nanoscale ◽  
2020 ◽  
Vol 12 (45) ◽  
pp. 22928-22934
Author(s):  
Cristina Palencia ◽  
Robert Seher ◽  
Jan Krohn ◽  
Felix Thiel ◽  
Felix Lehmkühler ◽  
...  
Keyword(s):  

In situ studies are crucial to demonstrate that magic-size clusters are always intermediates in the formation of regular NCs.


2012 ◽  
Vol 22 (6) ◽  
pp. 2485-2490 ◽  
Author(s):  
Yong-baek Lee ◽  
Seok Ho Lee ◽  
Kihyun Kim ◽  
Jin Woo Lee ◽  
Kyung-Yeon Han ◽  
...  

2015 ◽  
Vol 3 (27) ◽  
pp. 7128-7134 ◽  
Author(s):  
Nathaniel J. Carter ◽  
Roland Mainz ◽  
Bryce C. Walker ◽  
Charles J. Hages ◽  
Justus Just ◽  
...  
Keyword(s):  

Small (∼5 nm), Cu- and Sn-rich nanoparticles play a key role in initiating the growth of micrometer-sized Cu2ZnSn(S,Se)4 grains.


2018 ◽  
Vol 122 (50) ◽  
pp. 28550-28555 ◽  
Author(s):  
Yunong Zhang ◽  
Kaijie Ma ◽  
Xiangrong Kuang ◽  
Le Liu ◽  
Yunxu Sun ◽  
...  

2000 ◽  
Vol 47 (4A) ◽  
pp. 685-692 ◽  
Author(s):  
Noelle Lascoux ◽  
Guilhem Gallot ◽  
Francois Hache ◽  
Geoffrey Mitchell Gale ◽  
Savo Bratos ◽  
...  

2008 ◽  
Vol 129 (20) ◽  
pp. 204308 ◽  
Author(s):  
Ihar Shchatsinin ◽  
Tim Laarmann ◽  
Nick Zhavoronkov ◽  
Claus Peter Schulz ◽  
Ingolf V. Hertel

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