Asparaginyl Ligase-Catalyzed One-Step Cell Surface Modification of Red Blood Cells

2021 ◽  
Author(s):  
Thibault J. Harmand ◽  
Novalia Pishesha ◽  
Fabian B. H. Rehm ◽  
Weiyi Ma ◽  
William B. Pinney ◽  
...  
Keyword(s):  
Surface Modification ◽  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
One Step

Detection of islet cell surface antibodies by an indirect rosette assay, using islet cells and protein A-labeled sheep red blood cells

1984 ◽  
Vol 74 (1) ◽  
pp. 173-180 ◽  
Author(s):  
Shoji Maruyama ◽  
Masahiko Sugiura ◽  
Michio Nakazawa ◽  
Hiroko Tomiyama ◽  
Miyuki Shizawa ◽  
...  
Keyword(s):  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Islet Cell ◽  
Islet Cells ◽  
Protein A ◽  
Sheep Red Blood Cells ◽  
Islet Cell Surface Antibodies ◽  
Surface Antibodies

Improved Extraction of Saturated Fatty Acids but not Omega-3 Fatty Acids from Sheep Red Blood Cells Using a One-Step Extraction Procedure

Lipids ◽  
2012 ◽  
Vol 47 (7) ◽  
pp. 719-727 ◽  
Author(s):  
Edward H. Clayton ◽  
Catherine E. Gulliver ◽  
John W. Piltz ◽  
Robert D. Taylor ◽  
Robert J. Blake ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Saturated Fatty Acids ◽  
Extraction Procedure ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Sheep Red Blood Cells ◽  
One Step

scholarly journals Normal Functions of the Spleen Relative to Red Blood Cells: A Review

Blood ◽  
1959 ◽  
Vol 14 (4) ◽  
pp. 399-408 ◽  
Author(s):  
WILLIAM H. CROSBY
Keyword(s):  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Function ◽  
Red Cells ◽  
Red Cell ◽  
Cell Production ◽  
Normal Functions ◽  
Normal Spleen ◽  
And Storage

Abstract During all the stages of a red cell’s life the normal spleen exerts a normal function. Eight of these functions have been considered: (1) erythropoiesis; (2) an effect upon red cell production; (3) an effect upon maturation of the red cell surface; (4) the reservoir function; (5) the "culling function"; (6) iron turnover and storage; (7) the "pitting function"; (8) destruction of old red cells.

One-step enrichment of nucleated red blood cells

1993 ◽  
Vol 158 (2) ◽  
pp. 277-280 ◽  
Author(s):  
Neelima M. Bhat ◽  
Marcia M. Bieber ◽  
Nelson N.H. Teng
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Nucleated Red Blood Cells ◽  
One Step

Antigenically shielded universal red blood cells by polydopamine-based cell surface engineering

2014 ◽  
Vol 5 (9) ◽  
pp. 3463-3468 ◽  
Author(s):  
Ben Wang ◽  
Guangchuan Wang ◽  
Binjie Zhao ◽  
Jiajun Chen ◽  
Xueyun Zhang ◽  
...  
Keyword(s):  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surface Engineering ◽  
Cell Surface Engineering

A strategy of surface engineering by using polydopamine was developed to shelter antigens on red blood cells and prevent coagulation reactions.

scholarly journals Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity

1998 ◽  
Vol 141 (6) ◽  
pp. 1335-1347 ◽  
Author(s):  
Hui Qiao ◽  
Sandra L. Pelletier ◽  
Lucas Hoffman ◽  
Jill Hacker ◽  
R. Todd Armstrong ◽  
...  
Keyword(s):  
Cell Surface ◽  
Red Blood Cells ◽  
Conformational Change ◽  
Blood Cells ◽  
Ph Dependence ◽  
Coiled Coil ◽  
Low Ph ◽  
Cell Surface Expression ◽  
Fusion Activity ◽  
Influenza Hemagglutinin

We tested the role of the “spring-loaded” conformational change in the fusion mechanism of the influenza hemagglutinin (HA) by assessing the effects of 10 point mutants in the region of high coiled-coil propensity, HA2 54–81. The mutants included proline substitutions at HA2 55, 71, and 80, as well as a double proline substitution at residues 55 and 71. Mutants were expressed in COS or 293T cells and assayed for cell surface expression and structural features as well as for their ability to change conformation and induce fusion at low pH. We found the following: Specific mutations affected the precise carbohydrate structure and folding of the HA trimer. All of the mutants, however, formed trimers that could be expressed at the cell surface in a form that could be proteolytically cleaved from the precursor, HA0, to the fusion-permissive form, HA1-S-S-HA2. All mutants reacted with an antibody against the major antigenic site and bound red blood cells. Seven out of ten mutants displayed a wild-type (wt) or moderately elevated pH dependence for the conformational change. V55P displayed a substantial reduction (∼60– 80%) in the initial rate of lipid mixing. The other single mutants displayed efficient fusion with the same pH dependence as wt-HA. The double proline mutant V55P/ S71P displayed no fusion activity despite being well expressed at the cell surface as a proteolytically cleaved trimer that could bind red blood cells and change conformation at low pH. The impairment in fusion for both V55P and V55P/S71P was at the level of outer leaflet lipid mixing. We interpret our results in support of the hypothesis that the spring-loaded conformational change is required for fusion. An alternate model is discussed.

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