Antigenically shielded universal red blood cells by polydopamine-based cell surface engineering

2014 ◽  
Vol 5 (9) ◽  
pp. 3463-3468 ◽  
Author(s):  
Ben Wang ◽  
Guangchuan Wang ◽  
Binjie Zhao ◽  
Jiajun Chen ◽  
Xueyun Zhang ◽  
...  
Keyword(s):  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surface Engineering ◽  
Cell Surface Engineering

A strategy of surface engineering by using polydopamine was developed to shelter antigens on red blood cells and prevent coagulation reactions.

scholarly journals Surface-anchored framework for generating RhD-epitope stealth red blood cells

2020 ◽  
Vol 6 (12) ◽  
pp. eaaw9679 ◽  
Author(s):  
Yueqi Zhao ◽  
Mingjie Fan ◽  
Yanni Chen ◽  
Zhaoming Liu ◽  
Changyu Shao ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Blood Transfusion ◽  
Mouse Model ◽  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surface Engineering ◽  
Rabbit Model ◽  
Cell Surface Engineering ◽  
Alternative Approach

Rhesus D (RhD) is one of the most important immunogenic antigens on red blood cells (RBCs). However, the supply of RhD-negative blood frequently faces critical shortages in clinical practice, and the positive-to-negative transition of the RhD antigen remains a great challenge. Here, we developed an alternative approach for sheltering the epitopes on RhD-positive RBCs using a surface-anchored framework, which is flexible but can achieve an optimal shield effect with minimal physicochemical influence on the cell. The chemical framework completely obstructed the RhD antigens on the cell surface, and the assessments of both blood transfusion in a mouse model and immunostimulation with human RhD-positive RBCs in a rabbit model confirmed the RhD-epitope stealth characteristics of the engineered RBCs. This work provides an efficient methodology for improving the cell surface for universal blood transfusion and generally indicates the potential of rationally designed cell surface engineering for transfusion and transplantation medicine.

Detection of islet cell surface antibodies by an indirect rosette assay, using islet cells and protein A-labeled sheep red blood cells

1984 ◽  
Vol 74 (1) ◽  
pp. 173-180 ◽  
Author(s):  
Shoji Maruyama ◽  
Masahiko Sugiura ◽  
Michio Nakazawa ◽  
Hiroko Tomiyama ◽  
Miyuki Shizawa ◽  
...  
Keyword(s):  
Cell Surface ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Islet Cell ◽  
Islet Cells ◽  
Protein A ◽  
Sheep Red Blood Cells ◽  
Islet Cell Surface Antibodies ◽  
Surface Antibodies

scholarly journals Patterning of Growth Factor Gradients in Embryoid Bodies via Extracellular Matrix-Assisted Glycocalyx Engineering

2020 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
honigfort Daniel J. ◽  
purcell Sean C. ◽  
Kamil Godula
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Growth Factor ◽  
Cell Surface ◽  
Surface Engineering ◽  
Embryoid Bodies ◽  
Cell Surface Engineering

Cell surface engineering with synthetic glycomimetic co-receptors for FGF2 was used to establish gradients of stem cells with enhanced FGF2 affinity in embryoid bodies (EBs). Gradient shape was controlled by pre-assembly of glycomimetics into nanoscale vesicles with tunable dimensions and EB penetrance. <br>

Chemical and Enzymatic Strategies for Bacterial and Mammalian Cell Surface Engineering

2018 ◽  
Vol 24 (32) ◽  
pp. 8042-8050 ◽  
Author(s):  
Xiaobao Bi ◽  
Juan Yin ◽  
Ashley Chen Guanbang ◽  
Chuan-Fa Liu
Keyword(s):  
Cell Surface ◽  
Mammalian Cell ◽  
Surface Engineering ◽  
Cell Surface Engineering
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