Biocatalysis in Polymersomes: Improving Multienzyme Cascades with Incompatible Reaction Steps by Compartmentalization

ACS Catalysis ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3900-3904 ◽  
Author(s):  
Ludwig Klermund ◽  
Sarah T. Poschenrieder ◽  
Kathrin Castiglione
1980 ◽  
Vol 58 (24) ◽  
pp. 2594-2601 ◽  
Author(s):  
P. Stössel ◽  
G. Lazarovits ◽  
E. W. B. Ward

Intact 6-day old soybean hypocotyls (Glycine max L., cv. Altona) were inoculated with zoospores of Phytophthora megasperma Drechs. var. sojae Hildeb. either at the top (susceptible to compatible races, resistant to incompatible races) or the bottom (resistant to both compatible and incompatible races) and, after a 22-h incubation, were examined by light microscopy. Penetration at the top and bottom by both compatible (race 6) and incompatible (race 4) P. megasperma var. sojae was predominantly between anticlinal walls of epidermal cells. Both races, but especially race 4, also penetrated directly into the outer walls of epidermal cells, but epidermal cells rarely were invaded. Both races grew mainly intercellularly, but race 6 produced haustoria more frequently than race 4. Race 6 haustoria at the top of the hypocotyl were usually encased, those of race 4 were not. Growth of both races was equally dense in the first few cell layers, but the numbers of race 4 hyphae decreased rapidly while those of race 6 became more abundant in the deeper layers. With race 4, but not with race 6, most cells in the infected tissue were necrotic. Differences between the compatible and the incompatible interactions were not absolute; there were many unsuccessful invasion attempts by race 6 and individual hyphae of race 4 spread deeply into the tissue. At the bottom of the hypocotyl, growth of both races was more restricted. Race 6 produced fewer haustoria than at the top, and similarities to the incompatible reaction with race 4 at the top suggest that similar mechanisms of resistance may be involved.


2010 ◽  
Vol 102 (2) ◽  
pp. 569-577 ◽  
Author(s):  
Sun-Ju Shen ◽  
Sheng-Hung Wu ◽  
Jen-Hao Chi ◽  
Yih-Wen Wang ◽  
Chi-Min Shu

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 450b-450
Author(s):  
Patti Fashing-Burdette ◽  
James D. McCreight

In 1963, melon (Cucumis melo L.) plant introductions (PI) 124112 and PI 180280 were reported variable in response to inoculation with the T-1 or Freitag's isolates of watermelon mosaic virus. Most plants were symptomless, but some had small pinpoint necrotic lesions on cotyledons and leaves. The T-1 and Freitag's isolates of watermelon mosaic were later designated watermelon mosaic virus 1, and more recently renamed papaya ringspot virus watermelon strain (PRSV-W). When inoculated with California or Florida isolates of PRSV-W in 1993, WMR 29 a western U.S. shipping type melon derived from PI 180280 was symptomless (incompatible reaction) and SDS-immunodiffusion assays were negative. In contrast when inoculated with the same PRSV-W isolates, PI 124112 had incompatible reactions characterized by wilting, local lesions, systemic necrotic spots and necrosis and negative SDS-immunodiffusion assays.


protocols.io ◽  
2019 ◽  
Author(s):  
Rosangela Bevitori ◽  
Sanchari Sircar ◽  
Raquel neves ◽  
Roberto Coiti ◽  
Tiago Silva ◽  
...  

1981 ◽  
Vol 59 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Verna J. Higgins

Three races of Puccinia graminis tritici developed by successive mutations to give increased virulence (i.e., incompatible → mesothetic → compatible) on the Sr6 gene for resistance were compared histologically on cv. McMurachy (Sr6 gene) growing at 20.5 °C. The length of the longest hyphae and the size of the lesions remained similar in the three interactions up to 3 days after inoculation and then comparative degrees of growth occurred in the following decreasing order; compatible > mesothetic > incompatible. Necrotic cells in the compatible interaction were infrequent (maximum of 6% of infection sites) and hyphae radiated out from the point of ingress in an unimpeded manner. Although necrosis in the incompatible interaction occurred 1 day in advance of necrosis in the mesothetic reaction, by the 6th day, 98–100% of the infection sites in both systems had necrotic cells. In the mesothetic reaction, hyphae grew beyond the area of necrosis in a high proportion (70% at day 6) of the infection sites whereas the incidence of such growth was much less (8% at day 6) in the incompatible reaction. The role of the hypersensitive reaction in these interactions is discussed.


2020 ◽  
Vol 19 (3) ◽  
Author(s):  
R. Bevitori ◽  
S. Sircar ◽  
R.N. de Mello ◽  
R.C. Togawa ◽  
M.V.C.B. Côrtes ◽  
...  

2019 ◽  
Vol 28 (2) ◽  
pp. 195-209
Author(s):  
Mst Selina Momtaz ◽  
Shamim Shamsi ◽  
Tapan Kumar Dey

Bipolaris sorokiniana (Sacc.) Shoemaker is a phytopathogenic fungus, causal agent of leaf blight disease of wheat. This fungus is difficult to control because of its immense cultural, morphological, physiological, pathogenic and genetic variability. The aim of this investigation was to study the cultural variability of the selected isolates of B. sorokiniana by means of mycelial compatibility. One hundred fifty isolates of B. sorokiniana from eight districts (Dhaka, Gazipur, Dinajpur, Joypurhat, Pabna, Sirajgonj, Kushtia and Chuadanga) of Bangladesh were studied and cultural variation was characterized on the basis of colony color and texture on PDA medium. They were - Black-Mat (B-M), Black-Fluffy (B-F), Ash-Mat (A-M), Brownish Ash-Fluffy (Br. A-F), Blackish Ash-Mat (Bl.AM), Whitish Ash-Mat (W. A-M), Greenish Ash-Fluffy (G. A-F) and Pinkish White-Mat (P.W-M). To compare and understand cultural variability, mycelial compatibility test was done among the cultural groups. Results revealed that selected isolates of different cultural groups showed incompatible reaction. Incompatibility was also noticed between the isolates of same district and different districts. Dhaka Univ. J. Biol. Sci. 28(2): 195-209, 2019 (July)


2014 ◽  
Vol 116 (3) ◽  
pp. 1445-1452 ◽  
Author(s):  
Jing-Wei Tong ◽  
Wei-Chun Chen ◽  
Yun-Ting Tsai ◽  
Yan Cao ◽  
Jiann-Rong Chen ◽  
...  

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