scholarly journals Imprinted Particles for Direct Fluorescence Detection of Sialic Acid in Polar Media and on Cancer Cells with Enhanced Control of Nonspecific Binding

2021 ◽  
Author(s):  
Martha Kimani ◽  
Sarah Beyer ◽  
Zahra El-Schich ◽  
Kornelia Gawlitza ◽  
Anette Gjörloff-Wingren ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cancer Cells ◽  
Fluorescence Detection ◽  
Nonspecific Binding ◽  
Polar Media ◽  
Direct Fluorescence

Targeted Delivery of a Sialic Acid-Blocking Glycomimetic to Cancer Cells Inhibits Metastatic Spread

ACS Nano ◽  
2015 ◽  
Vol 9 (1) ◽  
pp. 733-745 ◽  
Author(s):  
Christian Büll ◽  
Thomas Jan Boltje ◽  
Eric A. W. van Dinther ◽  
Timo Peters ◽  
Annemarie M. A. de Graaf ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Metastatic Spread

Systematic Optimisation of Microtiter Plate Lectin Assay to Improve Sialic Acid Linkage Detection

2021 ◽  
Vol 24 ◽  
Author(s):  
Nur Hanina Izzati Khairol Mokhtar ◽  
Ainulkhir Hussin ◽  
Aidil Abdul Hamid ◽  
Shahrul Hisham Zainal Ariffin ◽  
Muhammad Ashraf Shahidan
Keyword(s):  
Sialic Acid ◽  
Specific Binding ◽  
Specific Antigen ◽  
Blocking Agent ◽  
Microtiter Plate ◽  
Tween 20 ◽  
Nonspecific Binding ◽  
Binding Assays ◽  
High Background ◽  
Sialic Acid Linkage

Aims: We aimed to develop a high-throughput lectin assay with minimized background signals to investigate the interactions of lectins and sialic acid glycans, focusing on prostate-specific antigen (PSA). Background: High background signals resulting from nonspecific binding are a significant concern for microtiter plate-based enzyme-linked lectin sorbent assays (ELLSAs), as they can mask specific binding signals and cause false-positive results. Methods: In this study, we constructed an ELLSA based on different washing step parameters, including the number of washing cycles, NaCl and Tween-20 concentrations, and the type of blocking agent and evaluated the effects on both specific and nonspecific binding signals. Furthermore, we performed a PSA binding assay using the optimized ELLSA. Results: The optimal washing parameters based on the highest specific binding signal proposed four cycles of washing steps using a washing buffer containing a high salt concentration (0.5 M NaCl) and mild detergent (0.05% Tween-20). The utilization of the optimized washing parameters in this assay was shown to be sufficient to obtain the optimal binding signals without the use of any blocking agent. Binding assays performed using the optimized ELLSA revealed that the glycan of the PSA sample used in this study mainly consists of terminal α2,6-linked sialic acid, as strongly recognized by Sambucus nigra agglutinin (SNA) with a KD value of 12.38 nM. Conclusion: The ELLSA reported in this study provides a simple yet sensitive assay for sialic acid linkage recognition.

Fluorescence measurements of peroxynitrite/peroxynitrous acid in cold air plasma treated aqueous solutions

2019 ◽  
Vol 21 (17) ◽  
pp. 8883-8896 ◽  
Author(s):  
Barbora Tarabová ◽  
Petr Lukeš ◽  
Malte U. Hammer ◽  
Helena Jablonowski ◽  
Thomas von Woedtke ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Chemical Kinetics ◽  
Fluorescence Detection ◽  
Peroxynitrous Acid ◽  
Air Plasma ◽  
Cold Air ◽  
Fluorescence Measurements ◽  
Direct Fluorescence ◽  
Kinetics Of

The first study providing direct fluorescence detection of peroxynitrite/peroxynitrous acid (ONOO−/ONOOH) in plasma activated liquids correlated with the chemical kinetics of ONOOH formation.

scholarly journals Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry

2020 ◽  
Vol 10 (3) ◽  
pp. 750 ◽  
Author(s):  
Megha Patel ◽  
Marek Feith ◽  
Birgit Janicke ◽  
Kersti Alm ◽  
Zahra El-Schich
Keyword(s):  
Breast Cancer ◽  
Sialic Acid ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cell Morphology ◽  
Molecularly Imprinted Polymers ◽  
Breast Cancer Cells ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Mcf 7

Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility.

Abstract 4558: 6-Nitroquinoline as a metabolically-activated probe for direct fluorescence detection of hypoxia

2010 ◽  
Author(s):  
Anuruddha Rajapakse ◽  
Ujjal Sarkar ◽  
Collette Linder ◽  
J Scott Daniels ◽  
Kent S. Gates
Keyword(s):  
Fluorescence Detection ◽  
Direct Fluorescence
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