Incorporation of Fluorescent Nonnatural Amino Acid into Sialic Acid-Binding Lectin for Fluorescence Detection of Ligand-Binding

2013 ◽  
Vol 86 (6) ◽  
pp. 729-735 ◽  
Author(s):  
Yasutaka Ito ◽  
Takahiro Hohsaka
Keyword(s):  
Sialic Acid ◽  
Amino Acid ◽  
Ligand Binding ◽  
Fluorescence Detection ◽  
Sialic Acid Binding ◽  
Nonnatural Amino Acid ◽  
Binding Lectin

Amino acid sequence of sialic acid binding lectin from frog (Rana catesbeiana) eggs

Biochemistry ◽  
1987 ◽  
Vol 26 (8) ◽  
pp. 2189-2194 ◽  
Author(s):  
Koiti Titani ◽  
Koji Takio ◽  
Manabu Kuwada ◽  
Kazuo Nitta ◽  
Fusao Sakakibara ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Rana Catesbeiana ◽  
Sialic Acid Binding ◽  
Binding Lectin

scholarly journals Chemical-modification studies of a unique sialic acid-binding lectin from the snail Achatina fulica. Involvement of tryptophan and histidine residues in biological activity

1988 ◽  
Vol 254 (1) ◽  
pp. 195-202 ◽  
Author(s):  
S Basu ◽  
C Mandal ◽  
A K Allen
Keyword(s):  
Biological Activity ◽  
Sialic Acid ◽  
Amino Acid ◽  
Fluorescence Emission ◽  
Amino Acid Residues ◽  
Achatina Fulica ◽  
Histidine Residues ◽  
Sialic Acid Binding ◽  
Arginine Residues ◽  
Binding Lectin

A unique sialic acid-binding lectin, achatininH (ATNH) was purified in single step from the haemolymph of the snail Achatina fulica by affinity chromatography on sheep submaxillary-gland mucin coupled to Sepharose 4B. The homogeneity was checked by alkaline gel electrophoresis, immunodiffusion and immunoelectrophoresis. Amino acid analysis showed that the lectin has a fairly high content of acidic amino acid residues (22% of the total). About 1.3% of the residues are half-cystine. The glycoprotein contains 21% carbohydrate. The unusually high content of xylose (6%) and fucose (2.7%) in this snail lectin is quite interesting. The protein was subjected to various chemical modifications in order to detect the amino acid residues and carbohydrate residues present in its binding sites. Modification of tyrosine and arginine residues did not affect the binding activity of ATNH; however, modification of tryptophan and histidine residues led to a complete loss of its biological activity. A marked decrease in the fluorescence emission was found as the tryptophan residues of ATNH were modified. The c.d. data showed the presence of an identical type of conformation in the native and modified agglutinin. The modification of lysine and carboxy residues partially diminished the biological activity. The activity was completely lost after a beta-elimination reaction, indicating that the sugars are O-glycosidically linked to the glycoprotein's protein moiety. This result confirms that the carbohydrate moiety also plays an important role in the agglutination property of this lectin.

scholarly journals Porcine Arterivirus Infection of Alveolar Macrophages Is Mediated by Sialic Acid on the Virus

2004 ◽  
Vol 78 (15) ◽  
pp. 8094-8101 ◽  
Author(s):  
Peter L. Delputte ◽  
Hans J. Nauwynck
Keyword(s):  
Sialic Acid ◽  
Alveolar Macrophages ◽  
Sialic Acids ◽  
Respiratory Syndrome Virus ◽  
Specific Monoclonal Antibody ◽  
Neuraminidase Treatment ◽  
Acid Binding Protein ◽  
Sialic Acid Binding ◽  
High Mannose ◽  
Binding Lectin

ABSTRACT Recently, we showed that porcine sialoadhesin (pSn) mediates internalization of the arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) in alveolar macrophages (Vanderheijden et al., J. Virol. 77:8207-8215, 2003). In rodents and humans, sialoadhesin, or Siglec-1, has been described as a macrophage-restricted molecule and to specifically bind sialic acid moieties. In the current study, we investigated whether pSn is a sialic acid binding protein and, whether so, whether this property is important for its function as a PRRSV receptor. Using untreated and neuraminidase-treated sheep erythrocytes, we showed that pSn binds sialic acid. Furthermore, pSn-specific monoclonal antibody 41D3, which blocks PRRSV infection, inhibited this interaction. PRRSV attachment to and infection of porcine alveolar macrophages (PAM) were both shown to be dependent on the presence of sialic acid on the virus: neuraminidase treatment of virus but not of PAM blocked infection and reduced attachment. Enzymatic removal of all N-linked glycans on the virus with N-glycosidase F reduced PRRSV infection, while exclusive removal of nonsialylated N-linked glycans of the high-mannose type with endoglycosidase H had no significant effect. Free sialyllactose and sialic acid containing (neo)glycoproteins reduced infection, while lactose and (neo)glycoproteins devoid of sialic acids had no significant effect. Studies with linkage-specific neuraminidases and lectins indicated that α2-3- and α2-6-linked sialic acids on the virion are important for PRRSV infection of PAM. From these results, we conclude that pSn is a sialic acid binding lectin and that interactions between sialic acid on the PRRS virion and pSn are essential for PRRSV infection of PAM.

scholarly journals Involvement of ER stress in apoptosis induced by sialic acid-binding lectin (leczyme) from bullfrog eggs

2013 ◽  
Vol 43 (6) ◽  
pp. 1799-1808 ◽  
Author(s):  
TAKEO TATSUTA ◽  
MASAHIRO HOSONO ◽  
YUKI MIURA ◽  
SHIGEKI SUGAWARA ◽  
YUKIKO KARIYA ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Er Stress ◽  
Sialic Acid Binding ◽  
Binding Lectin

A sialic acid-binding lectin from the legume Maackia fauriei: comparison with lectins from M. amurensis

Plant Science ◽  
2004 ◽  
Vol 167 (6) ◽  
pp. 1315-1321 ◽  
Author(s):  
Bum Soo Kim ◽  
Kyung Taik Oh ◽  
Due Hyeon Cho ◽  
Yun Jung Kim ◽  
Wan Mo Koo ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Sialic Acid Binding ◽  
Binding Lectin

scholarly journals Sialic acid-binding lectin from bullfrog eggs inhibits human malignant mesothelioma cell growth in vitro and in vivo

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190653 ◽  
Author(s):  
Takeo Tatsuta ◽  
Toshiyuki Satoh ◽  
Shigeki Sugawara ◽  
Akiyoshi Hara ◽  
Masahiro Hosono
Keyword(s):  
Sialic Acid ◽  
Cell Growth ◽  
Malignant Mesothelioma ◽  
Sialic Acid Binding ◽  
Mesothelioma Cell ◽  
Binding Lectin

Chemical Synthesis of a Synthetic Analogue of the Sialic Acid-Binding Lectin Siglec-7

ChemBioChem ◽  
2014 ◽  
Vol 15 (17) ◽  
pp. 2503-2507 ◽  
Author(s):  
Masayuki Izumi ◽  
Akihisa Otsuki ◽  
Mika Nishihara ◽  
Ryo Okamoto ◽  
Yasuhiro Kajihara
Keyword(s):  
Sialic Acid ◽  
Chemical Synthesis ◽  
Synthetic Analogue ◽  
Sialic Acid Binding ◽  
Binding Lectin
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