Hollow Pt Nanocage@Mesoporous SiO2 Nanoreactors as a Nanozyme for Colorimetric Immunoassays of Viral Diagnosis

Author(s):  
Lin Long ◽  
Mingji Zong ◽  
Yunqing Xie ◽  
Qing Chai ◽  
Jianbo Liu ◽  
...  
Author(s):  
S. E. Miller

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.


2021 ◽  
Vol 10 (1) ◽  
pp. 403-411
Author(s):  
Youliang Cheng ◽  
Mingjie Wang ◽  
Changqing Fang ◽  
Ying Wei ◽  
Jing Chen ◽  
...  

Abstract To change the optical properties and improve the antibacterial performances of carbon quantum dots (CQDs) and Ag NPs, mesoporous SiO2 spheres were combined with them to form the composites. In this paper, CQDs with a uniform size of about 3.74 nm were synthesized using glucose as carbon source. Then, CQDs/mesoporous SiO2/Ag NPs composites were obtained in situ under UV light irradiating by using mesoporous SiO2 and Ag NO3 as the carrier and silver resource, respectively. The diameter of CQDs/mesoporous SiO2/Ag NPs particles was in the range of 200–250 nm. With the increase in irradiating time, the red-shift in the UV-Vis spectrum for as-prepared CQDs/mesoporous SiO2/Ag NPs composites was found, and the adsorption peak was widened. In addition, the composites showed a high antibacterial activity against Staphylococcus aureus and Escherichia coli via disc diffusion method. These results indicated that inhibition circles for Ag NPs/mesoporous SiO2/CQDs and mesoporous SiO2/Ag NPs were similar in diameter. Furthermore, the two composites had a better bactericidal performance compared with other particles. Therefore, as-prepared CQDs/mesoporous SiO2/Ag NPs composites in this paper have great potential applications for fluorescent materials and antibacterial materials.


2015 ◽  
Vol 3 (15) ◽  
pp. 3708-3713 ◽  
Author(s):  
Du Yuan ◽  
Zibiao Li ◽  
Warintorn Thitsartarn ◽  
Xiaoshan Fan ◽  
Jiaotong Sun ◽  
...  

A simple and effective synthesis strategy is developed for electroactive β-PVDF-hfp where both intermolecular interaction and arrangement of surface functional groups operate.


2017 ◽  
Vol 9 (22) ◽  
pp. 19335-19344 ◽  
Author(s):  
Xin Ning ◽  
Yiyuan Lu ◽  
Heyun Fu ◽  
Haiqin Wan ◽  
Zhaoyi Xu ◽  
...  

2006 ◽  
Vol 18 (S1) ◽  
pp. 31-36
Author(s):  
Swantje Frühauf ◽  
Stefan E. Schulz ◽  
Thomas Gessner

1978 ◽  
Vol 7 (4) ◽  
pp. 368-371
Author(s):  
H M Friedman ◽  
C Koropchak

With the diminishing supply of the human fetal lung WI-38 cell strain, a replacement for viral isolation is needed. Two candidates are the human fetal lung strains MRC-5 and IMR-90. A comparison of WI-38, MRC-5, and IMR-90 was performed to evaluate efficiency and speed of viral isolation, clarity of cytophatic effect, and ease of growing the cells. The inocula were clinical specimens rather than tissue culture-adapted isolates. Frozen samples of 46 specimens that had previously yielded an isolate on WI-38 were thawed and inoculated onto WI-38, MRC-5, and IMR-90 cells. In addition, 95 freshly taken clinical specimens uf undetermined infectivity were inoculated onto the cell strains. Viral recovery rates were similar on all three strains, as were the appearance and speed of onset of the cytophatic effect. MRC-5 and WI-38 cells remained healthy until generation 36, whereas IMR-90 cells went into crisis by generation 20. The longer life span of the MRC-5 cells makes them more suitable than IMR-90 cells to replace the WI-38 strain for routine use in viral diagnosis.


1983 ◽  
Vol 67 (5) ◽  
pp. 953-972 ◽  
Author(s):  
Nathalie J. Schmidt
Keyword(s):  

2014 ◽  
Vol 26 (5) ◽  
pp. 1394-1396
Author(s):  
Qiuyue Lu ◽  
Zhigang Chen ◽  
Feng Chen ◽  
Xuan Huang ◽  
Zhengying Wu

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