Concurrent photothermal therapy and photodynamic therapy for cutaneous squamous cell carcinoma by gold nanoclusters under a single NIR laser irradiation

2019 ◽  
Vol 7 (44) ◽  
pp. 6924-6933 ◽  
Author(s):  
Pei Liu ◽  
Weitao Yang ◽  
Lei Shi ◽  
Haiyan Zhang ◽  
Yan Xu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Cell Carcinoma ◽  
Laser Irradiation ◽  
Squamous Cell ◽  
Photothermal Therapy ◽  
Gold Nanoclusters ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Single Drug ◽  
Nir Laser

The concurrent photothermal and photodynamic therapy of cutaneous squamous cell carcinoma by a single drug of Au25(Capt)18 nanoclusters is demonstrated, together with a preliminary immune response study conducted under a single NIR laser irradiation.

scholarly journals Folic acid functionalized hollow nanoparticles for selective photodynamic therapy of cutaneous squamous cell carcinoma

2019 ◽  
Vol 3 (6) ◽  
pp. 1113-1122 ◽  
Author(s):  
Duncan Hieu M. Dam ◽  
Lingzhi Zhao ◽  
Sophia A. Jelsma ◽  
Yanli Zhao ◽  
Amy S. Paller
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Folic Acid ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Zinc Phthalocyanine ◽  
Hollow Nanoparticles ◽  
Hollow Silica

Folic acid conjugated hollow silica nanocarriers loaded with zinc phthalocyanine are employed for selective photodynamic therapy of cutaneous squamous cell carcinoma.

scholarly journals An in vitro study of the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ying Ma ◽  
Shujuan Qu ◽  
Liangpeng Xu ◽  
Hongbo Lu ◽  
Baoguo Li
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Laser Irradiation ◽  
Squamous Cell ◽  
Protoporphyrin Ix ◽  
Control Group ◽  
Blank Control Group

Abstract Background The primary aim of this study was to observe the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma in vitro. Methods SCC25 cells were divided into the observation group and the blank control group. Different concentrations of 5-ALA and SCC25 cells were co-incubated for different times, and the concentration of protoporphyrin IX was detected by flow cytometry. SCC25 cells were divided into the 5-ALA group (100 mg/L), the laser irradiation group alone, the 5-ALA plus laser irradiation group, and the blank control group (0 mg/L 5-ALA), and the methyl thiazolyl tetrazolium (MTT) solution method was used (each group was incubated for 4, 8 and 12 h in turn). The cell survival rate was calculated. Using annexin V-fluorescein isothiocyanate/propidium iodide method, the apoptosis of SCC25 cells was detected by flow cytometry. Results The level of protoporphyrin IX in SCC25 cells increased with increased concentrations of 5-ALA and length of incubation. However, after 12 h, protoporphyrin IX level in SCC25 cells was gradually stabilized, and similar effect was obtained with 100 mg/L or more 5-ALA, indicating that the level of protoporphyrin IX in SCC25 cells was determined by 5-ALA concentration and incubation time. 5-ALA plus laser irradiation exerted an inhibitory effect on the growth of SCC25 cells, which was highly associated with drug dose and incubation time. Compared with the control group, laser irradiation alone or 5-ALA alone had no effect on the apoptosis of SCC25 cells. Different concentrations of 5-ALA combined with laser irradiation showed a remarkable effect of apoptosis, and a higher apoptosis rate was seen with higher drug concentrations. Conclusion 5-ALA-mediated photodynamic therapy affects the growth of SCC25 cells in vitro, which may provide a new idea for the clinical treatment of oral squamous cell carcinoma.

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