Spatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration

2019 ◽  
Vol 11 (9) ◽  
pp. 9557-9572 ◽  
Author(s):  
Wenlong Liu ◽  
Xiuli Dan ◽  
William W. Lu ◽  
Xiaoli Zhao ◽  
Changshun Ruan ◽  
...  
2016 ◽  
Vol 49 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Kartikey Grover ◽  
Liangjun Lin ◽  
Minyi Hu ◽  
Jesse Muir ◽  
Yi-Xian Qin

2016 ◽  
Vol 46 (5) ◽  
pp. 716-724 ◽  
Author(s):  
Kristin Morgenstern ◽  
Jens-Ulrich Polster ◽  
Doris Krabel

Knowledge of the ecology and biology of Rhabdocline pseudotsugae Sydow is still at a very early stage. Recent results indicate the existence of an endophytic stage of the fungus in the form of symptomless infections in various Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) tissue types. This study represents the first description of genetic variation between and within two populations of R. pseudotsugae. Needles featuring fruiting bodies of R. pseudotsugae were collected from sample areas in Saxony and North Rhine-Westphalia (in Germany), with different fungal genotypes distinguished using the start codon targeted polymorphism (SCoT) and branch point signal sequences (BPS) techniques. Five of the 20 primers tested could be selected for further investigation. A total of 349 fragments were amplified at an average of 69.8 fragments per primer. Results showed the two sample areas to exhibit a high degree of both genetic variability and genetic diversity. A clear differentiation between the sample areas was not observed. There was nevertheless a clear connection between the genetic distance and spatial distribution of the fruiting bodies of R. pseudotsugae within the sample areas themselves.


2018 ◽  
Vol 19 (4) ◽  
pp. 1326-1333 ◽  
Author(s):  
DURANTA D. KEMBAREN ◽  
ZAIRION ZAIRION ◽  
MOHAMMAD M. KAMAL ◽  
YUSLI WARDIATNO

Kembaren DD, Zairion, Kamal MM, Wardiatno Y. 2018. Abundance and spatial distribution of blue swimming crab (Portunuspelagicus) larvae during east monsoon in the East Lampung waters, Indonesia. Biodiversitas 19: 1326-1333. The blue swimmingcrab/BSC, Portunus pelagicus, is an economically important species in fishery industry and continuously being exploited, particularly inthe East Lampung waters. BSC research in Indonesia during the last decade was only restricted to the adult phase, while research ontheir larval dynamics in nature has not been done yet. This study aimed to assess the abundance and the distribution of BSC larvae, andto describe their correlation to its environmental conditions. This study was conducted in June 2017 (during east monsoon) in the EastLampung waters. Sampling was done in surface water during the daylight hours on nine sites with three replicates on each site. Planktonabundance and oceanographic profile were also recorded from the same sampling site. The result from this study showed that the waterquality from the environment was suitable for the development of BSC larvae. The abundance of the early-stage larvae (Z1-Z3) wasrelatively higher than the late-stage larvae (Z4 and M). The larval stages of BSC were dispersed and completed their development in themid-shore and offshore waters. There was a tendency that the early-stage was more abundant in the northern part, while the late-stagewas more abundant in the southern part of the study area. The highest larval abundance was found in the mid-shore, particularly in sitenumber five, which was influenced by the water mass density and current direction during the east monsoon. Moreover, oxygenconcentration and salinity of the water environment influenced the early-stage larvae abundance more than the late-stages abundance.


2021 ◽  
Author(s):  
Mahadeshwara Prasad ◽  
Somanna Ajjamada Nachappa ◽  
Niveditha Anand ◽  
Deepika Udayawara Rudresh ◽  
Yashika Singh ◽  
...  

AbstractHere we report for the first time the SARS-CoV-2 detection in autolysed samples from an exhumed decomposed body post-thirty six days after death. Both naso-oropharyngeal swabs and visceral samples from the lung, intestine, liver, and kidney were collected from the body exhumed post-fifteen days after burial, stored in viral transport medium and in saturated salt solution respectively. Naso-oropharyngeal swabs showed the presence of the SARS-CoV-2 genome as identified by the amplification of viral E, N, RdRP, or ORF1ab genes by RT-PCR. Subsequent examination of tissues reveal the detection of the virus genome in the intestine and liver, while no detection in the kidney and lung. These results signify the genome stability and implicate the virus survival in decomposed swab samples and in tissues and thereafter in storage solution. Further results also indicate spatial distribution of the virus in tissues during the early stage of infection in the subject with no respiratory distress. Considering the presence of cool, humid, and moist location of the exhumation, the presence of virus genome might also indicate that SARS-CoV-2 can persist for more than seven days on the surface of dead bodies similar to the Ebola virus, confirming that transmission from deceased subjects is possible for an extended period after death. These results further reaffirm the robustness of the RT-PCR aiding in the detection of viruses or their genome in decomposed samples when other methods of detection could not be useful.


2018 ◽  
Vol 38 (15) ◽  
Author(s):  
刘永华 LIU Yonghua ◽  
郑羽墨 ZHENG Yumo ◽  
阎雄飞 YAN Xiongfei ◽  
贺英 HE Ying ◽  
陆鹏飞 LU Pengfei

2014 ◽  
Vol 955-959 ◽  
pp. 3746-3756
Author(s):  
Ling Li Guo ◽  
Jin Man Wang ◽  
Zhong Ke Bai ◽  
Rui Xuan Yang ◽  
Yin Gui Cao

Quality of soil reconstruction is directly related to the vegetation recovery of opencast coal mine in loess area, therefore, research on soil properties of land reclamation has a very important role in mine ecological recovery. Based on an reclamation open coal mine dump loess area as the research area, semi-variance function as the basic tools, combined with traditional statistics and geo-statistics method, with statistical module of arcgis10.0, was applied to study the spatial variability of 0 ~ 40cm soil granules in early stage of reclamation, and using ordinary kriging method to draw the spatial distribution map of different particle size of soil granules. Results show that: (1) in early stage of reclamation, between 0 ~ 20 cm and 20 ~ 40 cm the component characteristic of different size of soil granules was similar, the average of silt content was all highest in the whole research region .(2) through the semi-variance function fitting, the theory model of semi-variogram of clay accord with spherical model, the semi-variance function of silt content in 0 ~ 20 cm level in line with the spherical model, in the 20 ~ 40 cm accord with the exponential model, the semi-variance model of sand in 0 ~ 20 cm fit with the spherical model, in the 20 ~ 40 cm semi-variance function in line with the exponential model, different size of soil granules content has spatial auto-correlation in a certain range, showing that has a medium spatial correlation. (3) the map can reveals the spatial distribution of soil granules in different levels, in 0 ~ 20 cm, on the horizontal direction of silt content in the high value area in the study area corresponds to the sand content is low in the area, both of them have good complementary, and in the vertical direction different soil granules content in the spatial distribution has no obvious regularity. This study can provide certain reference for loess area land reclamation of soil profile reconstruction and soil fertilization and amelioration.


2010 ◽  
Vol 2010.85 (0) ◽  
pp. _8-24_
Author(s):  
Ken NISHIKAWA ◽  
Hisashi NAITO ◽  
Takeshi MATSUMOTO ◽  
Masao TANAKA

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