Tailored Robust Hydrogel Composite Membranes for Continuous Protein Crystallization with Ultrahigh Morphology Selectivity

2018 ◽  
Vol 10 (31) ◽  
pp. 26653-26661 ◽  
Author(s):  
Lin Wang ◽  
Gaohong He ◽  
Xuehua Ruan ◽  
Daishuang Zhang ◽  
Wu Xiao ◽  
...  
Keyword(s):  
Protein Crystallization ◽  
Composite Membranes ◽  
Hydrogel Composite

scholarly journals Protein Crystallization in Ionic-Liquid Hydrogel Composite Membranes

Crystals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 253 ◽  
Author(s):  
Benny Danilo Belviso ◽  
Rosanna Caliandro ◽  
Shabnam Majidi Salehi ◽  
Gianluca Di Profio ◽  
Rocco Caliandro
Keyword(s):  
Ionic Liquid ◽  
Crystal Structures ◽  
Protein Crystallization ◽  
Composite Membranes ◽  
Glucose Isomerase ◽  
Biotechnological Applications ◽  
Comprehensive Overview ◽  
Structural Investigations ◽  
Hydrogel Composite ◽  
External Forces

Protein crystallization is a powerful purification tool. It is the first step for crystallographic structural investigations, and can be preparatory for biotechnological applications. However, crystallizing proteins is challenging and methods to control the crystallization process are needed. Ionic-liquid hydrogel composite membranes (IL-HCMs) have been used here as material capable of supporting protein crystallization and hosting grown crystals. We found that IL-HCMs affect the selection mechanism of glucose isomerase (GI) polymorphs and make GI crystals grow completely immersed into the hydrogel layer. X-ray diffraction studies show that IL ions do not bind to the protein, likely because IL molecules are constrained in the polymeric framework. Our GI crystal structures have been compared with many existing GI crystal structures using multivariate analysis tools, allowing a comprehensive overview of factors determining structural similarities, i.e., temperature variations and external stresses exerted during or after crystal growth, such as dehydration or presence of hydrogel of a different nature. GI crystals grown on IL-HCM fit perfectly in this framework, showing typical features induced by external forces. Overall, protein crystallization by IL-HCMs show potential for biotechnological applications, as it could constitute a natural means for containing crystallized enzymes in working conditions.

Fabric-hydrogel composite membranes for culturing microalgae in semipermeable membrane-based photobioreactors

2015 ◽  
Vol 54 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Seung-Yong Lee ◽  
Z-Hun Kim ◽  
Hwa Yeon Oh ◽  
Younghoon Choi ◽  
Hanwool Park ◽  
...  
Keyword(s):  
Composite Membranes ◽  
Semipermeable Membrane ◽  
Hydrogel Composite

Nanofibrous hydrogel composite membranes with ultrafast transport performance for molecular separation in organic solvents

2019 ◽  
Vol 7 (33) ◽  
pp. 19269-19279 ◽  
Author(s):  
Yi Li ◽  
Eric Wong ◽  
Alexander Volodine ◽  
Chris Van Haesendonck ◽  
Kaisong Zhang ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Interfacial Polymerization ◽  
Composite Membranes ◽  
Hydrogel Composite ◽  
Molecular Separation ◽  
Solvent Permeation

A polyamide (PA) nanofilm was successfully fabricated on the nanofibrous hydrogel support via controlled interfacial polymerization (IP) and exhibited an unprecedented solvent permeation for various organic solvents.

scholarly journals Shell-Forming Stimulus-Active Hydrogel Composite Membranes: Concept and Modeling

Micromachines ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 541 ◽  
Author(s):  
Adrian Ehrenhofer ◽  
Thomas Wallmersperger
Keyword(s):  
Finite Element ◽  
Composite Membranes ◽  
Bending Stiffness ◽  
Dead Weight ◽  
Flexible Displays ◽  
Temperature Expansion ◽  
Hydrogel Composite ◽  
Drastic Increase ◽  
Shell Forming ◽  
Expansion Model

The swelling of active hydrogels combined with passive layers allows the design of shell-forming structures. A shell-like structure offers different properties than a flat structure, e.g., variations in bending stiffness across different directions. A drastic increase of the bending stiffness is favorable e.g., in rollable/flexible displays: in their unrolled form, they have to be stiff enough to resist bending due to dead weight. At the same time, they have to be flexible enough to be rolled-up. This can be achieved by shell-forming. In the current modeling and simulation work, we present a basic concept of combined active–passive composites and demonstrate how they form shells. As the example material class, we use hydrogels with isotropic swelling capabilities. We demonstrate how to model the combined mechanical behavior with the Temperature-Expansion-Model. Afterwards, we show numerical results obtained by Finite Element simulations. We conclude that the envisioned structure has a great potential for obtaining soft rollable sheets that can be stiffened by intrinsic activation.

scholarly journals Ionic Liquid Hydrogel Composite Membranes (IL-HCMs)

2019 ◽  
Vol 3 (2) ◽  
pp. 47 ◽  
Author(s):  
Shabnam Majidi Salehi ◽  
Rosangela Santagada ◽  
Stefania Depietra ◽  
Enrica Fontananova ◽  
Efrem Curcio ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
New Media ◽  
Membrane Surface ◽  
Ammonium Hydroxide ◽  
Composite Membranes ◽  
Surface Hydrophobicity ◽  
Hydrophobic Membrane ◽  
Hydrogel Composite ◽  
Gel Layer ◽  
Transmembrane Flux

In this work, novel hydrogel composites membranes comprising [2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide as monomer, N,N-methylene bisacrylamide as cross-linker, and 1-butyl-3-methylimidazolium hexafluorophosphate as ionic liquid additive, have been developed. Ionic liquid hydrogel composite membranes (IL-HCMs) were tested for membrane contactors applications, aiming to reduce surface hydrophobicity of the polypropylene support, to reduce wetting tendency due to interaction with hydrophobic foulants, while affecting salts rejection in desalination operation, because of the entrapment of ILs inside the porous mesh-like structure of the gel layer. Transmembrane flux comparable to the sole polypropylene support was observed for IL content > 1 wt.%. Furthermore, all IL membranes presented a larger rejection to sodium chloride than the PP support or the composites without ionic liquid inside. Although the overall transmembrane flux of IL-HCMs developed in this work is comparable with that of state of the art MD membranes, this study demonstrated that the strong hydrophilic hydrogel layer, with C.A. < 50° for IL content larger than 1 wt.%, serves as a stabilization coating, by providing the new media between the feed and the hydrophobic membrane surface, thus potentially controlling the diffusion of hydrophobic foulant molecules. This would result in a decrease in the membrane wetting and fouling aptitude.

Bioinspired Synthesis of CaCO3Superstructures through a Novel Hydrogel Composite Membranes Mineralization Platform: A Comprehensive View

2015 ◽  
Vol 28 (4) ◽  
pp. 610-616 ◽  
Author(s):  
Gianluca Di Profio ◽  
Shabnam Majidi Salehi ◽  
Rocco Caliandro ◽  
Pietro Guccione ◽  
Giovanni Nico ◽  
...  
Keyword(s):  
Composite Membranes ◽  
Hydrogel Composite ◽  
Comprehensive View ◽  
Bioinspired Synthesis

Hydrogel Composite Membranes Incorporating Iron Oxide Nanoparticles as Topographical Designers for Controlled Heteronucleation of Proteins

2018 ◽  
Vol 18 (6) ◽  
pp. 3317-3327 ◽  
Author(s):  
Shabnam Majidi Salehi ◽  
Ana C. Manjua ◽  
Benny D. Belviso ◽  
Carla A. M. Portugal ◽  
Isabel M. Coelhoso ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Composite Membranes ◽  
Oxide Nanoparticles ◽  
Hydrogel Composite
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