One-Step in Situ Detection of miRNA-21 Expression in Single Cancer Cells Based on Biofunctionalized MoS2 Nanosheets

2017 ◽  
Vol 10 (1) ◽  
pp. 350-360 ◽  
Author(s):  
Gerile Oudeng ◽  
Manting Au ◽  
Jingyu Shi ◽  
Chunyi Wen ◽  
Mo Yang
Keyword(s):  
Cancer Cells ◽  
Mos2 Nanosheets ◽  
One Step ◽  
Single Cancer ◽  
In Situ Detection

In Situ Detection of Live Cancer Cells by Using Bioprobes Based on Au Nanoparticles

Langmuir ◽  
2008 ◽  
Vol 24 (21) ◽  
pp. 12112-12115 ◽  
Author(s):  
Jaemoon Yang ◽  
Kilho Eom ◽  
Eun-Kyung Lim ◽  
Jinsung Park ◽  
Yoonah Kang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Au Nanoparticles ◽  
In Situ Detection

In situ detection of plasma exosomal microRNA for lung cancer diagnosis using duplex-specific nuclease and MoS2 nanosheets

The Analyst ◽  
2021 ◽  
Author(s):  
Zibo Gao ◽  
Huijie Yuan ◽  
Yanhua Mao ◽  
Lihua Ding ◽  
Clement Yaw Effah ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Early Diagnosis ◽  
Cancer Diagnosis ◽  
Mos2 Nanosheets ◽  
Diagnosis And Prognosis ◽  
Lung Cancer Diagnosis ◽  
In Situ Detection ◽  
Exosomal Microrna ◽  
The Ideal

MicroRNAs (miRNAs) encapsulated in tumor-derived exosomes are becoming the ideal biomarkers for the early diagnosis and prognosis of lung cancer. However, the accuracy and sensitivity are often hampered by the...

A general one-step approach for in situ decoration of MoS2 nanosheets with inorganic nanoparticles

2015 ◽  
Vol 3 (3) ◽  
pp. 1042-1048 ◽  
Author(s):  
Zehong Cheng ◽  
Benzhao He ◽  
Li Zhou
Keyword(s):  
Inorganic Nanoparticles ◽  
Mos2 Nanosheets ◽  
One Step ◽  
The One

Here we present a general and controllable protocol for the one-step synthesis of various MoS2–INP nanohybrids by employing carboxylic MoS2 nanosheets as a versatile support.

Profiling protein–protein interactions of single cancer cells with in situ lysis and co-immunoprecipitation

Lab on a Chip ◽  
2019 ◽  
Vol 19 (11) ◽  
pp. 1922-1928 ◽  
Author(s):  
Ji Young Ryu ◽  
Jihye Kim ◽  
Min Ju Shon ◽  
Jiashu Sun ◽  
Xingyu Jiang ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Target Proteins ◽  
Single Cancer

We developed a single-cell version of the co-immunoprecipitation (co-IP) analysis that examines the amount and protein–protein interactions of target proteins immunoprecipitated from individual cells.

Sustainable one-step synthesis of hierarchical microspheres of PEGylated MoS2 nanosheets and MoO3 nanorods: Their cytotoxicity towards lung and breast cancer cells

2017 ◽  
Vol 396 ◽  
pp. 8-18 ◽  
Author(s):  
Neeraj Kumar ◽  
Blassan Plackal Adimuriyil George ◽  
Heidi Abrahamse ◽  
Vyom Parashar ◽  
Jane Catherine Ngila
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mos2 Nanosheets ◽  
Hierarchical Microspheres ◽  
One Step

One-step competitive lateral flow biosensor running on an independent quantification system for smart phones based in-situ detection of trace Hg(II) in tap water

2017 ◽  
Vol 214 ◽  
pp. 169-175 ◽  
Author(s):  
Nan Cheng ◽  
Yuancong Xu ◽  
Kunlun Huang ◽  
Yuting Chen ◽  
Zhansen Yang ◽  
...  
Keyword(s):  
Tap Water ◽  
Lateral Flow ◽  
Smart Phones ◽  
One Step ◽  
In Situ Detection

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

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