scholarly journals Encapsulation of Autoinducer Sensing Reporter Bacteria in Reinforced Alginate-Based Microbeads

2017 ◽  
Vol 9 (27) ◽  
pp. 22321-22331 ◽  
Author(s):  
Ping Li ◽  
Mareike Müller ◽  
Matthew Wook Chang ◽  
Martin Frettlöh ◽  
Holger Schönherr
Keyword(s):  
Reporter Bacteria

Bioluminescent reporter bacteria detect contaminants in soil samples

1994 ◽  
Vol 45-46 (1) ◽  
pp. 731-740 ◽  
Author(s):  
Robert S. Burlage ◽  
Anthony V. Palumbo ◽  
Armin Heitzer ◽  
Gary Sayler
Keyword(s):  
Soil Samples ◽  
Reporter Bacteria

scholarly journals Vibrio alginolyticus influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11567
Author(s):  
Panida Paopradit ◽  
Natta Tansila ◽  
Komwit Surachat ◽  
Pimonsri Mittraparp-arthorn
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Parahaemolyticus ◽  
Extracellular Polymeric Substances ◽  
Capsular Polysaccharide ◽  
Regulatory Gene ◽  
Challenge Test ◽  
Great Ability ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Reporter Bacteria

Background Acute hepatopancreatic necrosis syndrome (AHPND) caused by Vibrio parahaemolyticus strain (VPAHPND) impacts the shrimp industry worldwide. With the increasing problem of antibiotic abuse, studies on quorum sensing (QS) system and anti-QS compounds bring potential breakthroughs for disease prevention and treatment. Methods In this study, the cell-free culture supernatant (CFCS) and its extract of V. alginolyticus BC25 were investigated for anti-QS activity against a reporter bacteria, Chromobacterium violaceum DMST46846. The effects of CFCS and/ or extract on motility, biofilm formation and extracellular polymeric substances (EPSs) of VPAHPND PSU5591 were evaluated. Moreover, the effects of V. alginolyticus BC25 on virulence of VPAHPND PSU5591 were investigated by shrimp challenge test. The potentially active anti-QS compounds presented in the extract and effect on gene expression of VPAHPND PSU5591 were identified. Results The CFCS of V. alginolyticus BC25 and its extract showed a significant anti-QS activity against the reporter bacteria as well as swimming and swarming motilities, biofilms, and EPSs production by VPAHPND PSU5591. Transcriptome analysis revealed that V. alginolyticus BC25 extract significantly reduced the flagella genes involved in biofilm formation and iron-controlled virulence regulatory gene of VPAHPND PSU5591. Whereas, the LuxR family transcriptional regulator gene, c-factor, a cell-cell signaling gene, and capsular polysaccharide were up-regulated. The potentially active anti-QS compounds identified in extract were Cyclo-(L-Leu-L-Pro), and Cyclo-(L-Phe-L-Pro). Furthermore, V. alginolyticus BC25 enhanced disease resistance against VPAHPND PSU5591 in tested shrimp larvae. Conclusion These findings suggest that V. alginolyticus BC25 could provide natural anti-QS and anti-biofilms compounds and has great ability to be used as biocontrol agent against VPAHPND infection in shrimp aquaculture.

scholarly journals Isolated Reporter Bacteria in Supramolecular Hydrogel Microwell Arrays

Langmuir ◽  
2017 ◽  
Vol 33 (31) ◽  
pp. 7799-7809 ◽  
Author(s):  
Ping Li ◽  
Xiaoqiu Dou ◽  
Chuanliang Feng ◽  
Mareike Müller ◽  
Matthew Wook Chang ◽  
...  
Keyword(s):  
Supramolecular Hydrogel ◽  
Reporter Bacteria

A lower limit of detection for atrazine was obtained using bioluminescent reporter bacteria via a lower incubation temperature

2012 ◽  
Vol 84 ◽  
pp. 221-226 ◽  
Author(s):  
Kun Jia ◽  
Evgeni Eltzov ◽  
Timothée Toury ◽  
Robert S. Marks ◽  
Rodica E. Ionescu
Keyword(s):  
Lower Limit ◽  
Incubation Temperature ◽  
Limit Of Detection ◽  
Reporter Bacteria
Sign in / Sign up

Export Citation Format

Share Document