scholarly journals Vibrio alginolyticus influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11567
Author(s):  
Panida Paopradit ◽  
Natta Tansila ◽  
Komwit Surachat ◽  
Pimonsri Mittraparp-arthorn
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Parahaemolyticus ◽  
Extracellular Polymeric Substances ◽  
Capsular Polysaccharide ◽  
Regulatory Gene ◽  
Challenge Test ◽  
Great Ability ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Reporter Bacteria

Background Acute hepatopancreatic necrosis syndrome (AHPND) caused by Vibrio parahaemolyticus strain (VPAHPND) impacts the shrimp industry worldwide. With the increasing problem of antibiotic abuse, studies on quorum sensing (QS) system and anti-QS compounds bring potential breakthroughs for disease prevention and treatment. Methods In this study, the cell-free culture supernatant (CFCS) and its extract of V. alginolyticus BC25 were investigated for anti-QS activity against a reporter bacteria, Chromobacterium violaceum DMST46846. The effects of CFCS and/ or extract on motility, biofilm formation and extracellular polymeric substances (EPSs) of VPAHPND PSU5591 were evaluated. Moreover, the effects of V. alginolyticus BC25 on virulence of VPAHPND PSU5591 were investigated by shrimp challenge test. The potentially active anti-QS compounds presented in the extract and effect on gene expression of VPAHPND PSU5591 were identified. Results The CFCS of V. alginolyticus BC25 and its extract showed a significant anti-QS activity against the reporter bacteria as well as swimming and swarming motilities, biofilms, and EPSs production by VPAHPND PSU5591. Transcriptome analysis revealed that V. alginolyticus BC25 extract significantly reduced the flagella genes involved in biofilm formation and iron-controlled virulence regulatory gene of VPAHPND PSU5591. Whereas, the LuxR family transcriptional regulator gene, c-factor, a cell-cell signaling gene, and capsular polysaccharide were up-regulated. The potentially active anti-QS compounds identified in extract were Cyclo-(L-Leu-L-Pro), and Cyclo-(L-Phe-L-Pro). Furthermore, V. alginolyticus BC25 enhanced disease resistance against VPAHPND PSU5591 in tested shrimp larvae. Conclusion These findings suggest that V. alginolyticus BC25 could provide natural anti-QS and anti-biofilms compounds and has great ability to be used as biocontrol agent against VPAHPND infection in shrimp aquaculture.

scholarly journals ScrG, a GGDEF-EAL Protein, Participates in Regulating Swarming and Sticking in Vibrio parahaemolyticus

2007 ◽  
Vol 189 (11) ◽  
pp. 4094-4107 ◽  
Author(s):  
Yun-Kyeong Kim ◽  
Linda L. McCarter
Keyword(s):  
Gene Expression ◽  
Biofilm Formation ◽  
Vibrio Parahaemolyticus ◽  
Capsular Polysaccharide ◽  
Colony Morphology ◽  
Flagellar Gene ◽  
Surface Mobility ◽  
New Gene ◽  
Synthesis And Degradation

ABSTRACT In this work, we describe a new gene controlling lateral flagellar gene expression. The gene encodes ScrG, a protein containing GGDEF and EAL domains. This is the second GGDEF-EAL-encoding locus determined to be involved in the regulation of swarming: the first was previously characterized and named scrABC (for “swarming and capsular polysaccharide regulation”). GGDEF and EAL domain-containing proteins participate in the synthesis and degradation of the nucleotide signal cyclic di-GMP (c-di-GMP) in many bacteria. Overexpression of scrG was sufficient to induce lateral flagellar gene expression in liquid, decrease biofilm formation, decrease cps gene expression, and suppress the ΔscrABC phenotype. Removal of its EAL domain reversed ScrG activity, converting ScrG to an inhibitor of swarming and activator of cps expression. Overexpression of scrG decreased the intensity of a 32P-labeled nucleotide spot comigrating with c-di-GMP standard, whereas overexpression of scrG Δ EAL enhanced the intensity of the spot. Mutants with defects in scrG showed altered swarming and lateral flagellin production and colony morphology (but not swimming motility); furthermore, mutation of two GGDEF-EAL-encoding loci (scrG and scrABC) produced cumulative effects on swarming, lateral flagellar gene expression, lateral flagellin production and colony morphology. Mutant analysis supports the assignment of the primary in vivo activity of ScrG to acting as a phosphodiesterase. The data are consistent with a model in which multiple GGDEF-EAL proteins can influence the cellular nucleotide pool: a low concentration of c-di-GMP favors surface mobility, whereas high levels of this nucleotide promote a more adhesive Vibrio parahaemolyticus cell type.

Insights into Bacterial Milk Spoilage with Particular Emphasis on the Roles of Heat-Stable Enzymes, Biofilms, and Quorum Sensing

2018 ◽  
Vol 81 (10) ◽  
pp. 1651-1660 ◽  
Author(s):  
LEI YUAN ◽  
FAIZAN A. SADIQ ◽  
METTE BURMØLLE ◽  
TONGJIE LIU ◽  
GUOQING HE
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Enzyme Production ◽  
Extracellular Polymeric Substances ◽  
Hydrolytic Enzymes ◽  
Heat Stability ◽  
Planktonic Cells ◽  
Psychrotrophic Bacteria ◽  
Effective Strategies ◽  
Heat Stable

ABSTRACT Milk spoilage caused by psychrotrophic bacteria and their heat-stable enzymes is a serious challenge for the dairy industry. In many studies, spoilage has been explored based on the simplistic view of undesirable enzymes produced by planktonic cells. Recently, biofilms and quorum sensing (QS) have been suggested as important factors in the deterioration of milk, which opens new avenues for investigation of the processes and challenges. Production and heat stability of enzymes are enhanced in biofilms, mainly because of inherent differences in physiological states and protective shielding by extracellular polymeric substances. QS plays a key role in modulating expression of hydrolytic enzymes and biofilm formation. To date, few studies have been conducted to investigate the complex interplays of enzyme production, biofilm formation, and QS. This review provides novel insights into milk spoilage with particular emphasis on the roles of biofilms and QS and summarizes potential effective strategies for controlling the spoilage of milk.

scholarly journals Quorum sensing regulation methods and their effects on biofilm in biological waste treatment systems: A review

2021 ◽  
Vol 16 (7) ◽  
Author(s):  
Zhuqiu Sun ◽  
Jinying Xi ◽  
Chunping Yang ◽  
Wenjie Cong
Keyword(s):  
Microbial Community ◽  
Quorum Sensing ◽  
Future Study ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Waste Treatment ◽  
Quorum Quenching ◽  
Aggregation Control ◽  
Biological Waste Treatment ◽  
State Of Art

AbstractQuorum sensing (QS) plays an important role in microbial aggregation control. Recently, the optimization of biological waste treatment systems by QS regulation gained an increasing attention. The effects of QS regulation on treatment performances and biofilm were frequently investigated. To understand the state of art of QS regulation, this review summarizes the methods of QS enhancement and QS inhibition in biological waste treatment systems. Typical QS enhancement methods include adding exogenous QS molecules, adding QS accelerants and cultivating QS bacteria, while typical QS inhibition methods include additions of quorum quenching (QQ) bacteria, QS-degrading enzymes, QS-degrading oxidants, and QS inhibitors. The specific improvements after applying these QS regulation methods in different treatment systems are concluded. In addition, the effects of QS regulation methods on biofilm in biological waste treatment systems are reviewed in terms of biofilm formation, extracellular polymeric substances production, microbial viability, and microbial community. In the end, the knowledge gaps in current researches are analyzed, and the requirements for future study are suggested.

scholarly journals Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaojun Zhong ◽  
Ranran Lu ◽  
Fuwen Liu ◽  
Jinjie Ye ◽  
Junyang Zhao ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Parahaemolyticus ◽  
Wide Spectrum ◽  
Cell Communication ◽  
Systematic Research ◽  
Global Regulator ◽  
Food Borne ◽  
Reporter Assays ◽  
Complex Regulatory Network

Vibrio parahaemolyticus is one of the most important food-borne pathogens that cause economic and public health problems worldwide. Quorum sensing (QS) is a way for the cell-cell communication between bacteria that controls a wide spectrum of processes and phenotypic behaviors. In this study, we performed a systematic research of LuxR family regulators in V. parahaemolyticus and found that they influence the bacterial growth and biofilm formation. We then established a QS reporter plasmid based on bioluminescence luxCDABE operon of Vibrio harveyi and demonstrated that several LuxR family regulators integrated into QS circuit in V. parahaemolyticus. Thereinto, a novel LuxR family regulator, named RobA, was identified as a global regulator by RNA-sequencing analyses, which affected the transcription of 515 genes in V. parahaemolyticus. Subsequent studies confirmed that RobA regulated the expression of the exopolysaccharides (EPS) synthesis cluster and thus controlled the biofilm formation. In addition, bioluminescence reporter assays showed that RobA plays a key role in the QS circuit by regulating the expression of opaR, aphA, cpsQ-mfpABC, cpsS, and scrO. We further demonstrated that the regulation of RobA to EPS and MfpABC depended on OpaR and CpsQ, which combined the QS signal with bis-(3′-5′)-cyclic dimeric GMP to construct a complex regulatory network of biofilm formation. Our data provided new insights into the bacterial QS mechanisms and biofilm formation in V. parahaemolyticus.

scholarly journals Role of capsular polysaccharide (CPS) in biofilm formation and regulation of CPS production by quorum-sensing inVibrio vulnificus

2013 ◽  
Vol 90 (4) ◽  
pp. 841-857 ◽  
Author(s):  
Kyung-Jo Lee ◽  
Jeong-A Kim ◽  
Won Hwang ◽  
Soon-Jung Park ◽  
Kyu-Ho Lee
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Capsular Polysaccharide

Is the Quorum Sensing Type AI-1 System of Acidithiobacillus ferrooxidans Involved in its Attachment to Mineral Surfaces?

2007 ◽  
Vol 20-21 ◽  
pp. 345-349
Author(s):  
Lina María Ruíz ◽  
Alex Gonzalez ◽  
Marine Frezza ◽  
Laurent Soulère ◽  
Yves Queneau ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Acidithiobacillus Ferrooxidans ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Acyl Chain ◽  
Bacterial Attachment ◽  
Mineral Surfaces ◽  
Gene Encoding ◽  
Biofilm Development ◽  
Chain Lengths

Biofilm development plays a pivotal role in the bioleaching process. The attachment of the acidophilic chemolithotrophic Acidithiobacillus ferrooxidans to mineral surfaces is mediated by extracellular polymeric substances (EPS) involved in biofilm development. Previous work suggests that EPS composition of A. ferrooxidans is adapted to the energy source and, accordingly, the bacterium must be able to sense the surface to which attachment occurs with the consequent triggering of the expression of different EPS-genes. Quorum sensing (QS) is recognized as one of the main regulators of biofilm formation. A. ferrooxidans possesses a functional QS type AI-1 system and the analysis of culture supernatants revealed us that this bacterium is able to synthesize nine different homoserine lactones (AHLs) whose acyl-chain lengths oscillate between 8 and 16 carbons and include an alcohol or a ketone function at the C3 position. The transcription levels of the afeI gene encoding for the AHL synthase are higher in cells grown in sulfur and thiosulfate media than in iron-grown cells, suggesting that biofilm formation in A. ferrooxidans would be regulated by the QS type AI-1 system. In the present study, the effect of several synthetic AHLs and analogues on the attachment of A. ferrooxidans to pyrite was analyzed. Preliminary results suggest that some of these molecules are changing the bacterial attachment to pyrite.

scholarly journals Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)

2017 ◽  
Vol 44 (3) ◽  
pp. 470-479
Author(s):  
Patricia López-León ◽  
Antonio Luna-González ◽  
Ruth Escamilla-Montes ◽  
María del Carmen Flores-Miranda ◽  
Jesús A. Fierro-Coronado ◽  
...  
Keyword(s):  
Causative Agent ◽  
Biofilm Formation ◽  
Vibrio Parahaemolyticus ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Commercial Farms ◽  
Whiteleg Shrimp ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Selection Of

Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND), was isolated from the hepatopancreas of moribund whiteleg shrimp of commercial farms from Guasave, Sinaloa, Mexico. The isolates were screened on thiosulfate citrate bile salt sucrose agar plates for the selection of green colonies and further characterized through PCR with AP3 primers, 89F/R primers, hemolysin genes, hemolytic and enzymatic activity, hydrophobicity, autoaggregation, and biofilm formation. Bioassays by immersion challenge were conducted to confirm the pathogenicity of selected bacterial strains. In addition, the LC50 was calculated for each isolate. All isolates (35) belonged to V. parahaemolyticus, but three isolates did not correspond to strains that cause AHPND since they were negative with 89F/R primers. All isolates were αhemolytic and showed biofilm formation (from moderate to strong). Isolates were hydrophobic or hydrophilic and showed high autoaggregation capacity. Eight strains did not kill shrimp and eleven were pathogenic, but differences in virulence were found among them perhaps due to α-hemolysis and differences in biofilm formation and hydrophobicity. Therefore, performed characterization may help to understand the pathogenicity of V. parahaemolyticus. Finally, results showed that smaller shrimp are less resistant to V. parahaemolyticus infection.

Regulation of Thermostable Direct Hemolysin and Biofilm Formation of Vibrio parahaemolyticus by Quorum-Sensing Genes luxM and luxS

2018 ◽  
Vol 75 (9) ◽  
pp. 1190-1197 ◽  
Author(s):  
Muhan Guo ◽  
Zhijia Fang ◽  
Lijun Sun ◽  
Dongfang Sun ◽  
Yaling Wang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Parahaemolyticus ◽  
Thermostable Direct Hemolysin

scholarly journals Role of Heat Shock Proteases in Quorum-Sensing-Mediated Regulation of Biofilm Formation by Vibrio Species

mBio ◽  
2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Kyung-Jo Lee ◽  
You-Chul Jung ◽  
Soon-Jung Park ◽  
Kyu-Ho Lee
Keyword(s):  
Heat Shock ◽  
Quorum Sensing ◽  
Cell Density ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Capsular Polysaccharide ◽  
Signaling Molecules ◽  
Master Regulators

ABSTRACTCapsular polysaccharide (CPS) is essential for the dispersal of biofilms formed by the pathogenic bacteriumVibrio vulnificus. CPS production is induced by the quorum-sensing (QS) master regulator SmcR when biofilms mature. However,V. vulnificusbiofilms formed under heat shock conditions did not exhibit the dispersion stage. Transcripts of the CPS gene cluster were at basal levels in the heat-exposed cell owing to reduced cellular levels of SmcR. At least two proteases induced by heat shock, ClpPA and Lon, were responsible for determining the instability of SmcR.In vitroandin vivoassays demonstrated that SmcR levels were regulated via proteolysis by these proteases, with preferential proteolysis of monomeric SmcR. Thus, CPS production was not induced by QS when bacteria were heat treated. Further studies performed with otherVibriospecies demonstrated that high temperature deactivated the QS circuits by increased proteolysis of their QS master regulators, thus resulting in alterations to the QS-regulated phenotypes, including biofilm formation.IMPORTANCEThe term "quorum-sensing mechanism" is used to describe diverse bacterial cell density-dependent activities that are achieved by sensing of the signaling molecules and subsequent signal transduction to the master regulators. These well-known bacterial regulatory systems regulate the expression of diverse virulence factors and the construction of biofilms in pathogenic bacteria. There have been numerous studies designed to control bacterial quorum sensing by using small molecules to antagonize the quorum-sensing regulatory components or to interfere with the signaling molecules. In the present study, we showed that the quorum-sensing regulatory circuits of pathogenicVibriospecies were deactivated by heat shock treatment via highly increased proteolysis of the master transcription factors. Our results showed a new mode of quorum deactivation which can be achieved under conditions of high but nonlethal temperature even if the ambient signaling molecules may reach the levels representing high cell density.

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