Development of Nanofiber Sponges-Containing Nerve Guidance Conduit for Peripheral Nerve Regeneration in Vivo

2017 ◽  
Vol 9 (32) ◽  
pp. 26684-26696 ◽  
Author(s):  
Binbin Sun ◽  
Zifei Zhou ◽  
Tong Wu ◽  
Weiming Chen ◽  
Dawei Li ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Guidance Conduit

scholarly journals Biomimetic nerve guidance conduit containing engineered exosomes of adipose-derived stem cells promotes peripheral nerve regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zheng Yang ◽  
Yang Yang ◽  
Yichi Xu ◽  
Weiqian Jiang ◽  
Yan Shao ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Function Evaluation ◽  
Functional Protein ◽  
Rat Sciatic Nerve ◽  
Nerve Guidance Conduit ◽  
Gastrocnemius Muscles

Abstract Background Efficient and stable delivery of neurotrophic factors (NTFs) is crucial to provide suitable microenvironment for peripheral nerve regeneration. Neurotrophin-3 (NT-3) is an important NTF during peripheral nerve regeneration which is scarce in the first few weeks of nerve defect. Exosomes are nanovesicles and have been served as promising candidate for biocarrier. In this work, NT-3 mRNA was encapsulated in adipose-derived stem cell (ADSC)-derived exosomes (ExoNT-3). These engineered exosomes were applied as NT-3 mRNA carrier and then were loaded in nerve guidance conduit (ExoNT-3-NGC) to bridge rat sciatic nerve defect. Method NT-3 mRNA was encapsulated in exosomes by forcedly expression of NT-3 mRNA in the donor ADSCs. ExoNT-3 were co-cultured with SCs in vitro; after 24 h of culture, the efficiency of NT-3 mRNA delivery was evaluated by qPCR, western blotting and ELISA. Then, ExoNT-3 were loaded in alginate hydrogel to construct the nerve guidance conduits (ExoNT-3-NGC). ExoNT-3-NGC were implanted in vivo to reconstruct 10 mm rat sciatic nerve defect. The expression of NT-3 was measured 2 weeks after the implantation operation. The sciatic nerve functional index (SFI) was examined at 2 and 8 weeks after the operation. Moreover, the therapeutic effect of ExoNT-3-NGC was also evaluated by morphology assay, immunofluorescence staining of regenerated nerves, function evaluation of gastrocnemius muscles after 8 weeks of implantation. Results The engineered exosomes could deliver NT-3 mRNA to the recipient cells efficiently and translated into functional protein. The constructed NGC could realize stable release of exosomes at least for 2 weeks. After NGC implantation in vivo, ExoNT-3-NGC group significantly promote nerve regeneration and improve the function recovery of gastrocnemius muscles compared with control exosomes (Exoempty-NGC) group. Conclusion In this work, NGC was constructed to allow exosome-mediated NT-3 mRNA delivery. After ExoNT-3-NGC implantation in vivo, the level of NT-3 could restore which enhance the nerve regeneration. Our study provide a potential approach to improve nerve regeneration.

scholarly journals Biopolymer-nanotube nerve guidance conduit drug delivery for peripheral nerve regeneration: In vivo structural and functional assessment

2021 ◽  
Vol 6 (9) ◽  
pp. 2881-2893
Author(s):  
Ohan S. Manoukian ◽  
Swetha Rudraiah ◽  
Michael R. Arul ◽  
Jenna M. Bartley ◽  
Jiana T. Baker ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Functional Assessment ◽  
Peripheral Nerve Regeneration ◽  
Nerve Guidance Conduit

A Nerve Guidance Conduit Promoted Peripheral Nerve Regeneration in Rat

2020 ◽  
Author(s):  
Qingshan Long ◽  
Bingshan Wu ◽  
Yu Yang ◽  
Shanhong Wang ◽  
Yiwen Shen ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Guidance Conduit

scholarly journals In vivo assessment of peripheral nerve regeneration by diffusion tensor imaging

2011 ◽  
Vol 33 (3) ◽  
pp. 535-542 ◽  
Author(s):  
Shinsuke Morisaki ◽  
Yuko Kawai ◽  
Masahiro Umeda ◽  
Mayumi Nishi ◽  
Ryo Oda ◽  
...  
Keyword(s):  
Diffusion Tensor Imaging ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Diffusion Tensor ◽  
Peripheral Nerve Regeneration ◽  
In Vivo Assessment

scholarly journals Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

2018 ◽  
Vol 6 (5) ◽  
pp. 1059-1075 ◽  
Author(s):  
C. R. Carvalho ◽  
S. Wrobel ◽  
C. Meyer ◽  
C. Brandenberger ◽  
I. F. Cengiz ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Experimental Work ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Gellan Gum ◽  
In Vivo Study ◽  
Rat Sciatic Nerve

This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels.

Sign in / Sign up

Export Citation Format

Share Document