scholarly journals Complex Coacervation-Integrated Hybrid Nanoparticles Increasing Plasmid DNA Delivery Efficiency in Vivo

2016 ◽  
Vol 8 (45) ◽  
pp. 30735-30746 ◽  
Author(s):  
Yunfei Li ◽  
Brock Humphries ◽  
Zhishan Wang ◽  
Shuyao Lang ◽  
Xuefei Huang ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Hybrid Nanoparticles ◽  
Complex Coacervation ◽  
Delivery Efficiency

Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo

2004 ◽  
pp. 237-244
Author(s):  
Mark J. Jaroszeski ◽  
Loree C. Heller ◽  
Richard Gilbert ◽  
Richard Heller
Keyword(s):  
Solid Tumors ◽  
Plasmid Dna ◽  
Dna Delivery

scholarly journals Contactless magneto-permeabilization for intracellular plasmid DNA delivery in-vivo

2012 ◽  
Vol 8 (11) ◽  
pp. 1707-1713 ◽  
Author(s):  
Thomas J. Kardos ◽  
Dietmar P. Rabussay
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

scholarly journals Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo

Gene Therapy ◽  
2000 ◽  
Vol 7 (10) ◽  
pp. 826-829 ◽  
Author(s):  
L Heller ◽  
M J Jaroszeski ◽  
D Coppola ◽  
C Pottinger ◽  
R Gilbert ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Hepatocellular Carcinomas

scholarly journals Plasma-activated air mediates plasmid DNA delivery in vivo

2016 ◽  
Vol 3 ◽  
pp. 16028 ◽  
Author(s):  
Chelsea M Edelblute ◽  
Loree C Heller ◽  
Muhammad A Malik ◽  
Anna Bulysheva ◽  
Richard Heller
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

A comparison of plasmid DNA delivery efficiency and cytotoxicity of two cationic diblock polyoxazoline copolymers

2017 ◽  
Vol 28 (17) ◽  
pp. 175602 ◽  
Author(s):  
Roman Lehner ◽  
Kegang Liu ◽  
Xueya Wang ◽  
Marc Wolf ◽  
Patrick Hunziker
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Delivery Efficiency

scholarly journals Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube

2021 ◽  
Author(s):  
Shiori Nagahara ◽  
Tetsuya Higashiyama ◽  
Yoko Mizuta
Keyword(s):  
Tissue Culture ◽  
Germ Cells ◽  
Plasmid Dna ◽  
Genetically Modify ◽  
Pollen Tubes ◽  
Negative Control ◽  
Fluorescent Reporter ◽  
Biolistic Delivery ◽  
Delivery Efficiency

Abstract Key message Biolistic delivery into pollen. Abstract In recent years, genome editing techniques, such as the CRISPR/Cas9 system, have been highlighted as a new approach to plant breeding. Agrobacterium-mediated transformation has been widely utilized to generate transgenic plants by introducing plasmid DNA containing CRISPR/Cas9 into plant cells. However, this method has general limitations, such as the limited host range of Agrobacterium and difficulties in tissue culture, including callus induction and regeneration. To avoid these issues, we developed a method to genetically modify germ cells without the need for Agrobacterium-mediated transfection and tissue culture using tobacco as a model. In this study, plasmid DNA containing sequences of Cas9, guide RNA, and fluorescent reporter was introduced into pollen using a biolistic delivery system. Based on the transient expression of fluorescent reporters, the Arabidopsis UBQ10 promoter was found to be the most suitable promoter for driving the expression of the delivered gene in pollen tubes. We also evaluated the delivery efficiency in male germ cells in the pollen by expression of the introduced fluorescent marker. Mutations were detected in the target gene in the genomic DNA extracted from CRISPR/Cas9-introduced pollen tubes, but were not detected in the negative control. Bombarded pollen germinated pollen tubes and delivered their contents into the ovules in vivo. Although it is necessary to improve biolistic delivery efficiency and establish a method for the screening of genome-modified seeds, our findings provide important insights for the detection and production of genome-modified seeds by pollen biolistic delivery.

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery
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