Probe Intracellular Trafficking of a Polymeric DNA Delivery Vehicle by Functionalization with an Aggregation-Induced Emissive Tetraphenylethene Derivative

2015 ◽  
Vol 7 (51) ◽  
pp. 28494-28501 ◽  
Author(s):  
Xiongqi Han ◽  
Qixian Chen ◽  
Hongguang Lu ◽  
Jianbiao Ma ◽  
Hui Gao
Keyword(s):  
Intracellular Trafficking ◽  
Dna Delivery ◽  
Delivery Vehicle ◽  
Dna Delivery Vehicle

scholarly journals Attenuated Shigella as a DNA Delivery Vehicle for DNA-Mediated Immunization

Science ◽  
1995 ◽  
Vol 270 (5234) ◽  
pp. 299-303 ◽  
Author(s):  
D. R. Sizemore ◽  
A. A. Branstrom ◽  
J. C. Sadoff
Keyword(s):  
Dna Delivery ◽  
Delivery Vehicle ◽  
Dna Delivery Vehicle

scholarly journals Use of Native Lactococci as Vehicles for Delivery of DNA into Mammalian Epithelial Cells

2006 ◽  
Vol 72 (11) ◽  
pp. 7091-7097 ◽  
Author(s):  
Valéria Dellaretti Guimarães ◽  
Silvia Innocentin ◽  
François Lefèvre ◽  
Vasco Azevedo ◽  
Jean-Michel Wal ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammalian Cells ◽  
Vaccination Strategy ◽  
Dna Delivery ◽  
Human Colon ◽  
Eukaryotic Expression ◽  
Delivery Vehicle ◽  
Colon Carcinoma Cells ◽  
Dna Delivery Vehicle

ABSTRACT The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored the potential use of noninvasive L. lactis strains as a DNA delivery vehicle. We constructed two Escherichia coli-L. lactis shuttle plasmids, pLIG:BLG1 and pLIG:BLG2, containing a eukaryotic expression cassette with the cDNA of bovine β-lactoglobulin (BLG). The greatest BLG expression after transfection of Cos-7 cells was obtained with pLIG:BLG1, which was then used to transform L. lactis MG1363. The resulting L. lactis strain MG1363(pLIG:BLG1) was not able to express BLG. The potential of L. lactis as a DNA delivery vehicle was analyzed by detection of BLG in Caco-2 human colon carcinoma cells after 3 h of coincubation with (i) purified pLIG:BLG1, (ii) MG1363(pLIG:BLG1), (iii) a mix of MG1363(pLIG) and purified pLIG:BLG1, and (iv) MG1363. Both BLG cDNA and BLG expression were detected only in Caco-2 cells coincubated with MG1363(pLIG:BLG1). There was a decrease in the BLG cDNA level in Caco-2 cells between 24 and 48 h after coincubation. BLG expression by Caco-2 cells started at 24 h and increased between 24 and 72 h. BLG secretion by Caco-2 cells started 48 h after coincubation with MG1363(pLIG:BLG1). We conclude that lactococci can deliver BLG cDNA into mammalian epithelial cells, demonstrating their potential to deliver in vivo a DNA vaccine.

scholarly journals Intracellular Trafficking of Polyamidoamine–Poly(ethylene glycol) Block Copolymers in DNA Delivery

2011 ◽  
Vol 22 (8) ◽  
pp. 1519-1525 ◽  
Author(s):  
Daniel K. Bonner ◽  
Cheuk Leung ◽  
Jane Chen-Liang ◽  
Loice Chingozha ◽  
Robert Langer ◽  
...  
Keyword(s):  
Block Copolymers ◽  
Ethylene Glycol ◽  
Intracellular Trafficking ◽  
Dna Delivery ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1501
Author(s):  
Sarah Brendle ◽  
Nancy Cladel ◽  
Karla Balogh ◽  
Samina Alam ◽  
Neil Christensen ◽  
...  
Keyword(s):  
Critical Role ◽  
Specific Effect ◽  
Dna Delivery ◽  
Dna Uptake ◽  
In Vivo Models ◽  
Cottontail Rabbit ◽  
Species Specific ◽  
Dna Delivery Vehicle

Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models.

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery
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