Functionalized Carbon Quantum Dots with Dopamine for Tyrosinase Activity Monitoring and Inhibitor Screening: In Vitro and Intracellular Investigation

2015 ◽  
Vol 7 (42) ◽  
pp. 23564-23574 ◽  
Author(s):  
Lujing Chai ◽  
Jin Zhou ◽  
Hui Feng ◽  
Cong Tang ◽  
Yuanyuan Huang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots ◽  
Activity Monitoring ◽  
Tyrosinase Activity ◽  
Inhibitor Screening ◽  
Intracellular Investigation ◽  
Screening In Vitro

Phenylsulfonic acid functionalized carbon quantum dots based biosensor for acetylcholinesterase activity monitoring and inhibitor screening

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105454-105460 ◽  
Author(s):  
Fengqi Zhou ◽  
Hui Feng ◽  
Yafen Fang ◽  
Qian Sun ◽  
Zhaosheng Qian
Keyword(s):  
Quantum Dots ◽  
Ache Activity ◽  
Acetylcholinesterase Activity ◽  
Carbon Quantum Dots ◽  
Activity Monitoring ◽  
Inhibitor Screening

Phenylsulfonic acid functionalized carbon quantum dots (PSA-CQDs) were prepared and used to construct a convenient and reliable fluorometric biosensor for acetylcholinesterase (AChE) activity and inhibitor screening.

Preparation, characterization and in vitro anticancer performance of nanoconjugate based on carbon quantum dots and 5-Fluorouracil

2021 ◽  
Vol 120 ◽  
pp. 111781
Author(s):  
Elaine S.M. Cutrim ◽  
André A.M. Vale ◽  
Danilo Manzani ◽  
Hernane S. Barud ◽  
Enrique Rodríguez-Castellón ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots

A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots

RSC Advances ◽  
2016 ◽  
Vol 6 (76) ◽  
pp. 72670-72675 ◽  
Author(s):  
X. E. Zhao ◽  
C. H. Lei ◽  
Y. H. Wang ◽  
F. Qu ◽  
S. Y. Zhu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Graphene Quantum Dots ◽  
Tyrosinase Activity ◽  
Fluorometric Assay ◽  
Pristine Graphene ◽  
Fluorescence Sensing ◽  
Inhibitor Screening ◽  
Sensing Platform ◽  
First Time

Pristine graphene quantum dots (GQDs) without any functionalization were used as probes to develop a sensitive and selective fluorescence sensing platform for the detection of tyrosinase (TYR) activity and its inhibitor screening for the first time.

scholarly journals Quantifying Cytosolic Cytochrome c Concentration Using Carbon Quantum Dots as a Powerful Method for Apoptosis Detection

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1556
Author(s):  
Cristian Silviu Moldovan ◽  
Anca Onaciu ◽  
Valentin Toma ◽  
Radu Marginean ◽  
Alin Moldovan ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cytochrome C ◽  
Laser Scanning ◽  
A549 Cells ◽  
Carbon Quantum Dots ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Fluorescent Nanoparticles ◽  
Cyt C

Background: Cytochrome c (Cyt c) is a key biomarker for early apoptosis, and many methods were designed to detect its release from mitochondria. For a proper evaluation of these programed cell death mechanisms, fluorescent nanoparticles are excellent candidates due to their valuable optical properties. Among all classes of nanoparticles developed thus far, carbon-based quantum dots bring qualitative and efficient imaging strategies for biomedical applications as a consequence of their biocompatibility and low cytotoxicity. Methods: In this study, we synthesized carbon quantum dots smaller than 5 nm from sodium citrate and polyethylene imine. These nanoparticles were rigorously characterized, and their quenching capacity in apoptotic events was assessed in A549 cells treated with staurosporine and etoposide. For the evaluation of Cyt c release, a phenomenon directly correlated with apoptotic events, we ran a semiquantitative analysis using confocal laser scanning microscopy. Results: Carbon quantum dots were synthesized and were successfully employed for Cyt c detection by means of fluorescence microscopy. Significant drops in fluorescence intensity were observed in the case of cells treated with apoptosis-inducing therapeutic compounds compared to untreated cells, confirming Cyt c release from mitochondria to cytosol. Conclusion: Considering these results, we strongly believe this method can contribute to an indirect in vitro evaluation of apoptosis.

One-step synthesis of levodopa functionalized carbon quantum dots for selective detection of tyrosinase and inhibitor screening

2020 ◽  
Vol 159 ◽  
pp. 105456
Author(s):  
Saipeng Huang ◽  
Wenshuai Li ◽  
Xin Zhou ◽  
Mengyao Xie ◽  
Qing Luo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots ◽  
Selective Detection ◽  
Inhibitor Screening ◽  
One Step

A fluorometric assay for acetylcholinesterase activity and inhibitor screening with carbon quantum dots

2016 ◽  
Vol 222 ◽  
pp. 879-886 ◽  
Author(s):  
Zhaosheng Qian ◽  
Lujing Chai ◽  
Cong Tang ◽  
Yuanyuan Huang ◽  
Jianrong Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Acetylcholinesterase Activity ◽  
Carbon Quantum Dots ◽  
Fluorometric Assay ◽  
Inhibitor Screening

scholarly journals Kropki kwantowe węgla (CQD) przygotowane z biomasy odpadowej jako nowa klasa biomateriałów o właściwościach luminescencyjnych

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Łukasz JANUS ◽  
Marek PIĄTKOWSKI ◽  
Julia RADWAN-PRAGŁOWSKA ◽  
Aleksandra SIERAKOWSKA
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots

Kropki kwantowe węgla (Carbon Quantum Dots – CQD) to obiekty o rozmiarze mniejszym niż 10 nm, które mają zdolność emitowania promieniowania w zakresie widzialnym od od niebieskiego do czerwonego w zależności od zastosowanego promieniowaniawzbudzenia. Kropki kwantowe stosuje się w bioobrazowaniu in vitro struktur komórkowych lub tworzeniu bioczujników. W przeciwieństwie do klasycznych nanodotów, które są otrzymywane z prostych siarczków, selenków lub tellurków metali, kropki kwantowwęgla są zbudowane z nietoksycznego, biokompatybilnego rdzenia węglowego, dzięki czemu możliwe jest zastosowanie kwantowychkropek węgla w bioobrazowanie struktur biologicznych in vitro lub in vivo przy minimalnym działaniu cytotoksycznym na komórkiCelem badań było uzyskanie nanodotów węglowych zdolnych do emitowania fluorescencji przy użyciu ligniny z biomasy odpadowejCQD sfunkcjonalizowano aminokwasami. Rezultatem prac było uzyskanie serii CQD o zaawansowanych właściwościach luminecencyjnych z zastosowaniem metod hydrotermalnych i mikrofalowych. Gotowe produkty badano pod kątem ich cytotoksyczności

Reversible Fluorescent Nanoswitch Based on Carbon Quantum Dots Nanoassembly for Real-Time Acid Phosphatase Activity Monitoring

2015 ◽  
Vol 87 (14) ◽  
pp. 7332-7339 ◽  
Author(s):  
Zhaosheng Qian ◽  
Lujing Chai ◽  
Qian Zhou ◽  
Yuanyuan Huang ◽  
Cong Tang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Acid Phosphatase ◽  
Real Time ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Carbon Quantum Dots ◽  
Activity Monitoring

scholarly journals Nitrogen doped carbon quantum dots demonstrate no toxicity under in vitro conditions in a cervical cell line and in vivo in Swiss albino mice

2019 ◽  
Vol 8 (3) ◽  
pp. 395-406 ◽  
Author(s):  
Vimal Singh ◽  
Sunayana Kashyap ◽  
Umakant Yadav ◽  
Anchal Srivastava ◽  
Ajay Vikram Singh ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Line ◽  
Biomedical Imaging ◽  
Carbon Quantum Dots ◽  
Nitrogen Doped ◽  
Cervical Cell ◽  
Potential Applications ◽  
Nitrogen Doped Carbon

Carbon quantum dots (CQDs) and their derivatives have potential applications in the field of biomedical imaging.

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