Noninvasive Three-Dimensional In Situ and In Vivo Characterization of Bioprinted Hydrogel Scaffolds Using the X-ray Propagation-Based Imaging Technique

2021 ◽  
Author(s):  
Liqun Ning ◽  
Ning Zhu ◽  
An Smith ◽  
Ajay Rajaram ◽  
Huishu Hou ◽  
...  
Keyword(s):  
Imaging Technique ◽  
Three Dimensional ◽  
X Ray ◽  
Hydrogel Scaffolds ◽  
In Vivo Characterization

Three-Dimensional Characterization of the Microstructure of Bioglass/Polyethylene Composite by X-Ray Microtomography

2007 ◽  
Vol 330-332 ◽  
pp. 503-506
Author(s):  
Xiao Wei Fu ◽  
Jie Huang ◽  
E.S. Thian ◽  
Serena Best ◽  
William Bonfield
Keyword(s):  
Spatial Distribution ◽  
Volume Fraction ◽  
Three Dimensional ◽  
X Ray ◽  
Polyethylene Composite ◽  
Bioactive Properties ◽  
Recent Developments ◽  
3D Information

A Bioglass® reinforced polyethylene (Bioglass®/polyethylene) composite has been prepared, which combines the high bioactivity of Bioglass® and the toughness of polyethylene. The spatial distribution of Bioglass® particles within the composite is important for the performance of composites in-vivo. Recent developments in X-ray microtomography (XμT) have made it possible to visualize internal and microstructural details with different X-ray absorbencies, nondestructively, and to acquire 3D information at high spatial resolution. In this study, the volume fraction and 3D spatial distribution of Bioglass® particles has been acquired quantitatively by XμT. The information obtained provides a foundation for understanding the mechanical and bioactive properties of the Bioglass®/polyethylene composites.

scholarly journals Microstructural characterization of a Canadian oil sand

2012 ◽  
Vol 49 (10) ◽  
pp. 1212-1220 ◽  
Author(s):  
Dinh Hong Doan ◽  
Pierre Delage ◽  
Jean François Nauroy ◽  
Anh Minh Tang ◽  
Souhail Youssef
Keyword(s):  
Three Dimensional ◽  
Microstructural Characterization ◽  
Water Layer ◽  
3D Image ◽  
Oil Sand ◽  
X Ray ◽  
Mcmurray Formation ◽  
Gas Expansion

The microstructure of oil sand samples extracted at a depth of 75 m from the estuarine Middle McMurray Formation (Alberta, Canada) has been investigated using high resolution three-dimensional (3D) X-ray microtomography (µCT) and cryo scanning electron microscopy (CryoSEM). µCT images evidenced some dense areas composed of highly angular grains surrounded by fluids, which are separated by larger pores full of gas. In dense areas, 3D image analysis provided porosity values comparable with in situ log data and macroscopic laboratory determinations, showing that they are representative of intact states. µCT hence provided some information on the morphology of the cracks and disturbance created by gas expansion. The CryoSEM technique, in which the sample is freeze fractured within the SEM chamber prior to observation, provided pictures in which the (frozen) bitumen clearly appears between the sand grains. No evidence of the existence of a thin connate water layer between grains and the bitumen, frequently mentioned in the literature, has been obtained. Bitumen appears to strongly adhere to the grains, with some grains being completely coated. The curved shape of some bitumen menisci suggests a bitumen wet behaviour.

scholarly journals Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

2021 ◽  
Author(s):  
ITAMAR NECKEL ◽  
Lucas F. de Castro ◽  
Flavia Callefo ◽  
Verônica Teixeira ◽  
Angelo Gobbi ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Green Energy ◽  
X Rays ◽  
Electrical Measurements ◽  
X Ray ◽  
Electrochemical Nucleation ◽  
Synchrotron Light

Abstract Shedding synchrotron light on microfluidic systems, exploring several contrasts in situ operando at the nanoscale, like X-ray fluorescence, diffraction, luminescence, and absorption, has the potential to reveal new properties and functionalities of materials across diverse areas, such as green energy, photonics, and nanomedicine. In this work, we present the micro-fabrication and characterization of a multifunctional polyester/glass sealed microfluidic device well-suited to combine with analytical X-ray techniques. The device consists of smooth microchannels patterned on glass, where three gold electrodes are deposited into the channels to serve in situ electrochemistry analysis or standard electrical measurements. It has been efficiently sealed through an ultraviolet-sensitive sticker-like layer based on a polyester film, and The burst pressure determined by pumping water through the microchannel(up to 0.22 MPa). Overall, the device has demonstrated exquisite chemical resistance to organic solvents, and its efficiency in the presence of biological samples (proteins) is remarkable. The device potentialities, and its high transparency to X-rays, have been demonstrated by taking advantage of the X-ray nanoprobe Carnaúba/Sirius/LNLS, by obtaining 2D X-ray nanofluorescence maps on the microchannel filled with water and after an electrochemical nucleation reaction. To wrap up, the microfluidic device characterized here has the potential to be employed in standard laboratory experiments as well as in situ and in vivo analytical experiments using a wide electromagnetic window, from infrared to X-rays, which could serve experiments in many branches of science.

scholarly journals Three-dimensional in vivo characterization of calcification in native valves and in Freestyle versus homograft aortic valves

2005 ◽  
Vol 130 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Giovanni Melina ◽  
Paramate Horkaew ◽  
Mohamed Amrani ◽  
Michael B. Rubens ◽  
Magdi H. Yacoub ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Aortic Valves ◽  
In Vivo Characterization

scholarly journals Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Itamar T. Neckel ◽  
Lucas F. de Castro ◽  
Flavia Callefo ◽  
Verônica C. Teixeira ◽  
Angelo L. Gobbi ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Green Energy ◽  
X Rays ◽  
Electrical Measurements ◽  
X Ray ◽  
Electrochemical Nucleation ◽  
Synchrotron Light

AbstractShedding synchrotron light on microfluidic systems, exploring several contrasts in situ/operando at the nanoscale, like X-ray fluorescence, diffraction, luminescence, and absorption, has the potential to reveal new properties and functionalities of materials across diverse areas, such as green energy, photonics, and nanomedicine. In this work, we present the micro-fabrication and characterization of a multifunctional polyester/glass sealed microfluidic device well-suited to combine with analytical X-ray techniques. The device consists of smooth microchannels patterned on glass, where three gold electrodes are deposited into the channels to serve in situ electrochemistry analysis or standard electrical measurements. It has been efficiently sealed through an ultraviolet-sensitive sticker-like layer based on a polyester film, and The burst pressure determined by pumping water through the microchannel(up to 0.22 MPa). Overall, the device has demonstrated exquisite chemical resistance to organic solvents, and its efficiency in the presence of biological samples (proteins) is remarkable. The device potentialities, and its high transparency to X-rays, have been demonstrated by taking advantage of the X-ray nanoprobe Carnaúba/Sirius/LNLS, by obtaining 2D X-ray nanofluorescence maps on the microchannel filled with water and after an electrochemical nucleation reaction. To wrap up, the microfluidic device characterized here has the potential to be employed in standard laboratory experiments as well as in in situ and in vivo analytical experiments using a wide electromagnetic window, from infrared to X-rays, which could serve experiments in many branches of science.

scholarly journals Isolation of PCV3 from Perinatal and Reproductive Cases of PCV3-Associated Disease and In Vivo Characterization of PCV3 Replication in CD/CD Growing Pigs

Viruses ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 219 ◽  
Author(s):  
Juan Mora-Díaz ◽  
Pablo Piñeyro ◽  
Huigang Shen ◽  
Kent Schwartz ◽  
Fabio Vannucci ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Virus Production ◽  
Growing Pigs ◽  
Porcine Circovirus ◽  
Experimental Conditions ◽  
Histological Lesion ◽  
In Vivo Characterization

Porcine circovirus 3 (PCV3) has been identified as a putative swine pathogen with a subset of infections resulting in stillborn and mummified fetuses, encephalitis and myocarditis in perinatal, and periarteritis in growing pigs. Three PCV3 isolates were isolated from weak-born piglets or elevated stillborn and mummified fetuses. Full-length genome sequences from different passages and isolates (PCV3a1 ISU27734, PCV3a2 ISU58312, PCV3c ISU44806) were determined using metagenomics sequencing. Virus production in cell culture was confirmed by qPCR, IFA, and in situ hybridization. In vivo replication of PCV3 was also demonstrated in CD/CD pigs (n = 8) under experimental conditions. Viremia, first detected at 7 dpi, was detected in all pigs by 28 dpi. IgM antibody response was detected between 7–14 dpi in 5/8 PCV3-inoculated pigs but no IgG seroconversion was detected throughout the study. Pigs presented histological lesion consistent with multi systemic inflammation characterized by myocarditis and systemic perivasculitis. Viral replication was confirmed in all tissues by in situ hybridization. Clinically, all animals were unremarkable throughout the study. Although the clinical relevance of PCV3 remains under debate, this is the first isolation of PCV3 from perinatal and reproductive cases of PCV3-associated disease and in vivo characterization of PCV3 infection in a CD/CD pig model.

scholarly journals 3D-printed jars for ball-milling experiments monitoredin situby X-ray powder diffraction

2017 ◽  
Vol 50 (4) ◽  
pp. 994-999 ◽  
Author(s):  
Nikolay Tumanov ◽  
Voraksmy Ban ◽  
Agnieszka Poulain ◽  
Yaroslav Filinchuk
Keyword(s):  
Powder Diffraction ◽  
Materials Science ◽  
Three Dimensional ◽  
High Energy ◽  
X Ray ◽  
3D Printed ◽  
Background Absorption ◽  
Design And Manufacture

Mechanochemistry is flourishing in materials science, but a characterization of the related processes is difficult to achieve. Recently, the use of plastic jars in shaker mills has enabledin situX-ray powder diffraction studies at high-energy beamlines. This paper describes an easy way to design and manufacture these jars by three-dimensional (3D) printing. A modified wall thickness and the use of a thin-walled sampling groove and a two-chamber design, where the milling and diffraction take place in two communicating volumes, allow for a reduced background/absorption and higher angular resolution, with the prospect for use at lower-energy beamlines. 3D-printed polylactic acid jars show good mechanical strength and they are also more resistant to solvents than jars made of polymethyl methacrylate. The source files for printing the jars are available as supporting information.

scholarly journals In situ Characterization of Laser Powder Bed Fusion Using High-Speed Synchrotron X-ray Imaging Technique

2019 ◽  
Vol 25 (S2) ◽  
pp. 2566-2567 ◽  
Author(s):  
Niranjan Parab ◽  
Cang Zhao ◽  
Ross Cunningham ◽  
Luis I. Escano ◽  
Kamel Fezzaa ◽  
...  
Keyword(s):  
High Speed ◽  
Imaging Technique ◽  
Powder Bed Fusion ◽  
Laser Powder Bed Fusion ◽  
In Situ Characterization ◽  
Powder Bed ◽  
X Ray ◽  
X Ray Imaging

IN-VIVO CHARACTERIZATION OF BIOLOGICAL CONTROLLED-RELEASE DEVICE FOR IN-SITU DELIVERY OF TGF-??1 FOR ORTHOPEDIC TISSUE ENGINEERING

ASAIO Journal ◽  
2005 ◽  
Vol 51 (2) ◽  
pp. 17A
Author(s):  
Hyun J Paek ◽  
Anelisa B Campaner ◽  
Jeffrey R Morgan ◽  
Roy K Aaron ◽  
Deborah M Ciombor ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Controlled Release ◽  
Release Device ◽  
In Vivo Characterization
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