Improving Chronic Diabetic Wound Healing through an Injectable and Self-Healing Hydrogel with Platelet-Rich Plasma Release

2020 ◽  
Vol 12 (50) ◽  
pp. 55659-55674
Author(s):  
Zhiyong Qian ◽  
Haiping Wang ◽  
Yating Bai ◽  
Yuqing Wang ◽  
Lei Tao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Platelet Rich Plasma ◽  
Self Healing ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

scholarly journals Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration: Erratum

Theranostics ◽  
2021 ◽  
Vol 11 (20) ◽  
pp. 10174-10175
Author(s):  
Chenggui Wang ◽  
Min Wang ◽  
Tianzhen Xu ◽  
Xingxing Zhang ◽  
Cai Lin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Regeneration ◽  
Self Healing ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

scholarly journals Platelet-Rich Plasma with Endothelial Progenitor Cells Accelerates Diabetic Wound Healing in Rats by Upregulating the Notch1 Signaling Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Cheng Zhang ◽  
Yu Zhu ◽  
Shengdi Lu ◽  
Wanrun Zhong ◽  
Yanmao Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Signaling Pathway ◽  
Platelet Rich Plasma ◽  
Diabetic Wound ◽  
Endothelial Progenitor ◽  
Notch1 Signaling ◽  
Diabetic Wound Healing ◽  
Notch1 Signaling Pathway ◽  
Diabetic Wounds

Diabetic wounds, as a kind of refractory wound, are very difficult to heal. Both endothelial progenitor cell (EPC) transplantation and platelet-rich plasma (PRP) can improve diabetic wound healing to some extent. However, PRP application cannot provide reparative cells, while EPC transplantation cannot replenish the required growth factors for wound healing. Thus, when applied alone, neither of these factors is sufficient for effective wound healing. Furthermore, the proliferation, differentiation, and fate of the transplanted EPCs are not well known. Therefore, in this study, we examined the efficacy of combined PRP application with EPC transplantation in diabetic wound healing. Our results indicated that PRP application improved EPC proliferation and migration. The Notch signaling pathway plays a key role in the regulation of the proliferation and differentiation of stem cells and angiogenesis in wound healing. The application of PRP upregulated the Notch pathway-related gene and protein expression in EPCs. Furthermore, experiments with shNotch1-transfected EPCs indicated that PRP enhanced the function of EPCs by upregulating the Notch1 signaling pathway. In vivo studies further indicated that the combination of PRP and EPC transplantation increased neovascularization, reduced wound size, and improved healing in rat wound models. Thus, PRP application can provide the necessary growth factors for wound healing, while EPC transplantation offers the required cells, indicating that the combination of both is a potent novel approach for treating diabetic wounds.

scholarly journals Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration

Theranostics ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 65-76 ◽  
Author(s):  
Chenggui Wang ◽  
Min Wang ◽  
Tianzhen Xu ◽  
Xingxing Zhang ◽  
Cai Lin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Regeneration ◽  
Self Healing ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

scholarly journals Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

2021 ◽  
Author(s):  
Xuejun Ni ◽  
Xiuying Shan ◽  
Lili Xu ◽  
Wenjun Yu ◽  
Mingliang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Closure Rate ◽  
Expression Levels ◽  
Sd Rats ◽  
Diabetic Wound Healing

Abstract Background: Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing.Methods: ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence and immunohistochemistry were performed to observe the changes in neovascularization. ELISA and Western blot were utilized to detect the angiogenesis-related protein expression levels. The proliferation of endothelial cells was assessed by the MTS assay.Results: ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP and negative control. The expression levels of VEGF, p-STAT3 and SDF-1 in the ADSC-PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP.Conclusions: PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization.

scholarly journals Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

2020 ◽  
Author(s):  
Xuejun Ni ◽  
Xiuying Shan ◽  
Lili Xu ◽  
Wenjun Yu ◽  
Mingliang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Promoting Effect ◽  
Closure Rate ◽  
Sd Rats ◽  
Diabetic Wound Healing

Abstract Background: Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. Methods: ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence and immunohistochemistry were performed to observe the changes in neovascularization. The proliferation of endothelial cells was assessed by the MTS assay. Results: ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP and negative control. The expression levels of VEGF and SDF-1 in the ADSC-PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP. Conclusions: PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization. Keywords: Adipose-derived stem cell, Platelet-rich plasma, Diabetic wound healing, Neovascularization.

scholarly journals Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nesrine Ebrahim ◽  
Arigue A. Dessouky ◽  
Ola Mostafa ◽  
Amira Hassouna ◽  
Mohamed M. Yousef ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Notch Signaling ◽  
Platelet Rich Plasma ◽  
Combined Therapy ◽  
Notch Pathway ◽  
Notch Signaling Pathway ◽  
Diabetic Wound ◽  
Epidermal Thickness ◽  
Diabetic Wound Healing

Abstract Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality, and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to the rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose-derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups [control (unwounded), sham (wounded but non-diabetic), diabetic, PRP-treated, ADSC-treated, and PRP+ADSCs-treated groups]. The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen, and angiogenesis. Moreover, gene and protein expression of key elements of the Notch signaling pathway (Notch1, Delta-like canonical Notch ligand 4 (DLL4), Hairy Enhancer of Split-1 (Hes1), Hey1, Jagged-1), gene expression of angiogenic marker (vascular endothelial growth factor and stromal cell-derived factor 1) and epidermal stem cells (EPSCs) related gene (ß1 Integrin) were assessed. Results Our data showed better wound healing of PRP+ADSCs compared to their individual use after 7 and 14 days as the combined therapy caused reepithelialization and granulation tissue formation with a marked increase in area percentage of collagen, epidermal thickness, and angiogenesis. Moreover, Notch signaling was significantly downregulated, and EPSC proliferation and recruitment were enhanced compared to other treated groups and diabetic groups. Conclusions These data demonstrated that PRP and ADSCs combined therapy significantly accelerated healing of diabetic wounds induced experimentally in rats via modulating the Notch pathway, promoting angiogenesis and EPSC proliferation.

scholarly journals Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xuejun Ni ◽  
Xiuying Shan ◽  
Lili Xu ◽  
Wenjun Yu ◽  
Mingliang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Closure Rate ◽  
Expression Levels ◽  
Sd Rats ◽  
Diabetic Wound Healing

Abstract Background Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. Methods ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were performed to observe the changes in neovascularization. ELISA and Western blot were utilized to detect the angiogenesis-related protein expression levels. The proliferation of endothelial cells was assessed by the MTS assay. Results ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP, and negative control. The expression levels of VEGF, p-STAT3, and SDF-1 in the ADSC+PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP. Conclusions PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization.

scholarly journals Adipose Mesenchymal Stem Cells Combined with Platelet Rich Plasma Accelerate Diabetic Wound Healing by Modulating Notch Pathway

2021 ◽  
Author(s):  
Nesrine Ebrahim ◽  
Arigue Dessouky ◽  
Ola Mostafa ◽  
Mohamed Yousef ◽  
Yasmin Seleem ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Notch Signaling ◽  
Platelet Rich Plasma ◽  
Combined Therapy ◽  
Notch Pathway ◽  
Notch Signaling Pathway ◽  
Diabetic Wound ◽  
Epidermal Thickness ◽  
Diabetic Wound Healing

Abstract Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups (control, sham, diabetic, PRP-treated, ADSCs-treated and PRP+ADSCs-treated groups). The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen and angiogenesis. Moreover, gene and protein expression of key elements of Notch signaling pathway (Notch1, Delta like canonical Notch ligand 4 (DLL4), Hairy Enhancer of Split-1 (Hes1), Hey1, Jagged-1), gene expression of angiogenic marker (Vascular endothelial growth factor & stromal cell-derived factor 1) and epidermal stem cells (EPSCs) related gene (ß1 Integrin) were assessed. Results Our data showed a strong wound-healing effect of PRP+ADSCs compared to their individual use after 7 and 14 days. Combined therapy caused marked increase in area percentage of collagen, epidermal thickness and angiogenesis. Moreover, Notch signaling was significantly down-regulated, EPSCs proliferation and recruitment was enhanced compared to other treated groups and diabetic group. Conclusions These data demonstrated that PRP and ADSCs combined therapy significantly accelerated healing of diabetic wounds induced experimentally in rats via modulating Notch pathway, promoting angiogenesis and EPSCs proliferation.

Sign in / Sign up

Export Citation Format

Share Document