Silanized Luminescent Quantum Dots for the Simultaneous Multicolor Lateral Flow Immunoassay of Two Mycotoxins

2020 ◽  
Vol 12 (22) ◽  
pp. 24575-24584 ◽  
Author(s):  
Olga A. Goryacheva ◽  
Chris Guhrenz ◽  
Kristian Schneider ◽  
Natalia V. Beloglazova ◽  
Irina Yu. Goryacheva ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Luminescent Quantum Dots

scholarly journals Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 768
Author(s):  
Hyung-Mo Kim ◽  
Chiwoo Oh ◽  
Jaehyun An ◽  
Seungki Baek ◽  
Sungje Bock ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Limit Of Detection ◽  
Specific Binding ◽  
Calf Serum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Uniform Size ◽  
Highly Sensitive ◽  
Wide Range ◽  
New Biomarkers

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs.

scholarly journals Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite

2017 ◽  
Vol Volume 12 ◽  
pp. 4805-4812 ◽  
Author(s):  
Yan Zhao ◽  
Qiang Zhang ◽  
Qingfeng Meng ◽  
Fenglian Wu ◽  
Lihua Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Image Analysis ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Ige Antibody

A Lateral Flow Immunoassay for Prostate‐Specific Antigen Detection Using Silica‐Coated CdSe @ ZnS Quantum Dots

2020 ◽  
Vol 41 (10) ◽  
pp. 989-993
Author(s):  
Sungje Bock ◽  
Jaehyun An ◽  
Hyung‐Mo Kim ◽  
Jaehi Kim ◽  
Heung‐Su Jung ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

Fluorescently labelled multiplex lateral flow immunoassay based on cadmium-free quantum dots

Methods ◽  
2017 ◽  
Vol 116 ◽  
pp. 141-148 ◽  
Author(s):  
Natalia V. Beloglazova ◽  
Aleksander M. Sobolev ◽  
Mickael D. Tessier ◽  
Zeger Hens ◽  
Irina Yu. Goryacheva ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

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