A Lateral Flow Immunoassay for Prostate‐Specific Antigen Detection Using Silica‐Coated CdSe @ ZnS Quantum Dots

2020 ◽  
Vol 41 (10) ◽  
pp. 989-993
Author(s):  
Sungje Bock ◽  
Jaehyun An ◽  
Hyung‐Mo Kim ◽  
Jaehi Kim ◽  
Heung‐Su Jung ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 33
Author(s):  
Sungje Bock ◽  
Hyung-Mo Kim ◽  
Jaehi Kim ◽  
Jaehyun An ◽  
Yun-Sik Choi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Silica Nanoparticles ◽  
Prostate Specific Antigen ◽  
False Negative ◽  
Specific Antigen ◽  
Calf Serum ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay

Prostate cancer can be detected early by testing the presence of prostate-specific antigen (PSA) in the blood. Lateral flow immunoassay (LFIA) has been used because it is cost effective and easy to use and also has a rapid sample-to-answer process. Quantum dots (QDs) with very bright fluorescence have been previously used to improve the detection sensitivity of LFIAs. In the current study, a highly sensitive LFIA kit was devised using QD-embedded silica nanoparticles. In the present study, only a smartphone and a computer software program, ImageJ, were used, because the developed system had high sensitivity by using very bright nanoprobes. The limit of PSA detection of the developed LFIA system was 0.138 ng/mL. The area under the curve of this system was calculated as 0.852. The system did not show any false-negative result when 47 human serum samples were analyzed; it only detected PSA and did not detect alpha-fetoprotein and newborn calf serum in the samples. Additionally, fluorescence was maintained on the strip for 10 d after the test. With its high sensitivity and convenience, the devised LFIA kit can be used for the diagnosis of prostate cancer.

scholarly journals Silver-Assembled Silica Nanoparticles in Lateral Flow Immunoassay for Visual Inspection of Prostate-Specific Antigen

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 4099
Author(s):  
Hyung-Mo Kim ◽  
Jaehi Kim ◽  
Sungje Bock ◽  
Jaehyun An ◽  
Yun-Sik Choi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Silica Nanoparticles ◽  
Prostate Specific Antigen ◽  
Cancer Diagnosis ◽  
Specific Antigen ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Clinical Samples ◽  
Important Indicator ◽  
Prostate Cancer Diagnosis

Prostate-specific antigen (PSA) is the best-known biomarker for early diagnosis of prostate cancer. For prostate cancer in particular, the threshold level of PSA <4.0 ng/mL in clinical samples is an important indicator. Quick and easy visual detection of the PSA level greatly helps in early detection and treatment of prostate cancer and reducing mortality. In this study, we developed optimized silica-coated silver-assembled silica nanoparticles (SiO2@Ag@SiO2 NPs) that were applied to a visual lateral flow immunoassay (LFIA) platform for PSA detection. During synthesis, the ratio of silica NPs to silver nitrate changed, and as the synthesized NPs exhibited distinct UV spectra and colors, most optimized SiO2@Ag@SiO2 NPs showed the potential for early prostate cancer diagnosis. The PSA detection limit of our LFIA platform was 1.1 ng/mL. By applying each SiO2@Ag@SiO2 NP to the visual LFIA platform, optimized SiO2@Ag@SiO2 NPs were selected in the test strip, and clinical samples from prostate cancer patients were successfully detected as the boundaries of non-specific binding were clearly seen and the level of PSA was <4 ng/mL, thus providing an avenue for quick prostate cancer diagnosis and early treatment.

Quantitative Lateral Flow Immunoassay for Total Prostate Specific Antigen in Serum

2015 ◽  
Vol 49 (4) ◽  
pp. 579-588 ◽  
Author(s):  
I. P. Andreeva ◽  
V. G. Grigorenko ◽  
A. M. Egorov ◽  
A. P. Osipov
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Total Prostate Specific Antigen

Real-time prostate-specific antigen detection with prostate-specific antigen imprinted capacitive biosensors

2015 ◽  
Vol 891 ◽  
pp. 120-129 ◽  
Author(s):  
Gizem Ertürk ◽  
Martin Hedström ◽  
M. Aşkın Tümer ◽  
Adil Denizli ◽  
Bo Mattiasson
Keyword(s):  
Real Time ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Detection

scholarly journals Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 768
Author(s):  
Hyung-Mo Kim ◽  
Chiwoo Oh ◽  
Jaehyun An ◽  
Seungki Baek ◽  
Sungje Bock ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Limit Of Detection ◽  
Specific Binding ◽  
Calf Serum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Uniform Size ◽  
Highly Sensitive ◽  
Wide Range ◽  
New Biomarkers

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs.

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