Locked Nucleic Acid Nanomicelle with Cell-Penetrating Peptides for Glutathione-Triggered Drug Release and Cell Fluorescence Imaging

2018 ◽  
Vol 2 (1) ◽  
pp. 370-377 ◽  
Author(s):  
Xiaoting Ji ◽  
Ruiyuan Zhang ◽  
Zhenbo Wang ◽  
Shuyan Niu ◽  
Caifeng Ding
Keyword(s):  
Nucleic Acid ◽  
Drug Release ◽  
Fluorescence Imaging ◽  
Cell Penetrating Peptides ◽  
Locked Nucleic Acid ◽  
Cell Penetrating ◽  
Triggered Drug Release

Nucleic Acid Nanocapsules for Enzyme-Triggered Drug Release

2017 ◽  
Vol 139 (18) ◽  
pp. 6278-6281 ◽  
Author(s):  
Joseph K. Awino ◽  
Saketh Gudipati ◽  
Alyssa K. Hartmann ◽  
Joshua J. Santiana ◽  
Dominic F. Cairns-Gibson ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Drug Release ◽  
Triggered Drug Release

Efficient Intracellular Delivery of Nucleic Acid Pharmaceuticals Using Cell-Penetrating Peptides

2011 ◽  
Vol 45 (7) ◽  
pp. 1132-1139 ◽  
Author(s):  
Ikuhiko Nakase ◽  
Hidetaka Akita ◽  
Kentaro Kogure ◽  
Astrid Gräslund ◽  
Ülo Langel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating

scholarly journals Nucleic acid delivery by cell penetrating peptides derived from dengue virus capsid protein: design and mechanism of action

FEBS Journal ◽  
2013 ◽  
Vol 281 (1) ◽  
pp. 191-215 ◽  
Author(s):  
João M. Freire ◽  
Ana Salomé Veiga ◽  
Inês Rego de Figueiredo ◽  
Beatriz G. de la Torre ◽  
Nuno C. Santos ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dengue Virus ◽  
Capsid Protein ◽  
Protein Design ◽  
Mechanism Of Action ◽  
Cell Penetrating Peptides ◽  
Nucleic Acid Delivery ◽  
Virus Capsid ◽  
Cell Penetrating ◽  
Virus Capsid Protein

Insulin-loaded liposomes functionalized with cell-penetrating peptides: influence on drug release and permeation through porcine nasal mucosa

2021 ◽  
pp. 126624
Author(s):  
Eliete de Souza Von Zuben ◽  
Josimar Oliveira Eloy ◽  
Victor Hugo Sousa Araujo ◽  
Maria Palmira Daflon Gremião ◽  
Marlus Chorilli
Keyword(s):  
Drug Release ◽  
Nasal Mucosa ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating

scholarly journals Internalisation and Biological Activity of Nucleic Acids Delivering Cell-Penetrating Peptide Nanoparticles Is Controlled by the Biomolecular Corona

2021 ◽  
Vol 14 (7) ◽  
pp. 667
Author(s):  
Annely Lorents ◽  
Maria Maloverjan ◽  
Kärt Padari ◽  
Margus Pooga
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Transfection Efficiency ◽  
Protein Corona ◽  
Blood Stream ◽  
Cell Penetrating Peptides ◽  
Biological Efficiency ◽  
Cell Penetrating

Nucleic acid molecules can be transferred into cells to alter gene expression and, thus, alleviate certain pathological conditions. Cell-penetrating peptides (CPPs) are vectors that can be used for transfecting nucleic acids as well as many other compounds. CPPs associate nucleic acids non-covalently, forming stable nanoparticles and providing efficient transfection of cells in vitro. However, in vivo, expected efficiency is achieved only in rare cases. One of the reasons for this discrepancy is the formation of protein corona around nanoparticles, once they are exposed to a biological environment, e.g., blood stream. In this study, we compared protein corona of CPP-nucleic acid nanoparticles formed in the presence of bovine, murine and human serum. We used Western blot and mass-spectrometry to identify the major constituents of protein corona forming around nanoparticles, showing that proteins involved in transport, haemostasis and complement system are its major components. We investigated physical features of nanoparticles and measured their biological efficiency in splice-correction assay. We showed that protein corona constituents might alter the fate of nanoparticles in vivo, e.g., by subjecting them to phagocytosis. We demonstrated that composition of protein corona of nanoparticles is species-specific that leads to dissimilar transfection efficiency and should be considered while developing delivery systems for nucleic acids.

scholarly journals Internalisation and biological activity of nucleic acids delivering cell-penetrating peptide nanoparticles is controlled by the biomolecular corona

2021 ◽  
Author(s):  
Annely Lorents ◽  
Maria Maloverjan ◽  
Kärt Padari ◽  
Margus Pooga
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Transfection Efficiency ◽  
Protein Corona ◽  
Blood Stream ◽  
Cell Penetrating Peptides ◽  
Biological Efficiency ◽  
Cell Penetrating

Nucleic acid molecules can be transferred into cells to alter gene expression and, thus, alleviate certain pathological conditions. Cell-penetrating peptides (CPPs) are vectors that can be used for transfecting nucleic acids as well as many other compounds. CPPs associate nucleic acids non-covalently, forming stable nanoparticles and providing efficient transfection of cells in vitro. However, in vivo, expected efficiency is achieved only in rare cases. One of the reasons for this discrepancy is formation of protein corona around nanoparticles, once they are exposed to a biological environment, e.g. blood stream. In this study, we compared CPP-nucleic acid nanoparticles formed in the presence of bovine, murine and human serum. We used Western blot and mass-spectrometry to identify the major constituents of protein corona forming around nanoparticles, showing that proteins involved in transport, haemostasis and complement system are its major components. We investigated physical features of nanoparticles, and measured their biological efficiency in splice-correction assay. We showed that protein corona constituents might alter the fate of nanoparticles in vivo, e.g. by subjecting them to phagocytosis. We demonstrated that composition of protein corona of nanoparticles is species-specific that leads to dissimilar transfection efficiency and should be taken into account while developing delivery systems for nucleic acids.

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