Efficient Intracellular Delivery of Nucleic Acid Pharmaceuticals Using Cell-Penetrating Peptides

2011 ◽  
Vol 45 (7) ◽  
pp. 1132-1139 ◽  
Author(s):  
Ikuhiko Nakase ◽  
Hidetaka Akita ◽  
Kentaro Kogure ◽  
Astrid Gräslund ◽  
Ülo Langel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating

Evaluation of Cell-Penetrating Peptides (CPPs) as Vehicles for Intracellular Delivery of Antisense Peptide Nucleic Acid (PNA)

2006 ◽  
Vol 17 (3) ◽  
pp. 750-758 ◽  
Author(s):  
Nadia Bendifallah ◽  
Frank Winther Rasmussen ◽  
Vladimir Zachar ◽  
Peter Ebbesen ◽  
Peter E. Nielsen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Antisense Peptide

scholarly journals Novel human-derived cell-penetrating peptides for specific subcellular delivery of therapeutic biomolecules

2005 ◽  
Vol 390 (2) ◽  
pp. 407-418 ◽  
Author(s):  
Catherine de Coupade ◽  
Antonio Fittipaldi ◽  
Vanessa Chagnas ◽  
Matthieu Michel ◽  
Sophie Carlier ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mammalian Cells ◽  
Heparan Sulphate ◽  
Intracellular Delivery ◽  
Membrane Lipid ◽  
Cell Penetrating Peptides ◽  
Heparin Binding ◽  
Independent Manner ◽  
Cell Penetrating

Short peptide sequences that are able to transport molecules across the cell membrane have been developed as tools for intracellular delivery of therapeutic molecules. This work describes a novel family of cell-penetrating peptides named Vectocell® peptides [also termed DPVs (Diatos peptide vectors)]. These peptides, originating from human heparin binding proteins and/or anti-DNA antibodies, once conjugated to a therapeutic molecule, can deliver the molecule to either the cytoplasm or the nucleus of mammalian cells. Vectocell® peptides can drive intracellular delivery of molecules of varying molecular mass, including full-length active immunoglobulins, with efficiency often greater than that of the well-characterized cell-penetrating peptide Tat. The internalization of Vectocell® peptides has been demonstrated to occur in both adherent and suspension cell lines as well as in primary cells through an energy-dependent endocytosis process, involving cell-membrane lipid rafts. This endocytosis occurs after binding of the cell-penetrating peptides to extracellular heparan sulphate proteoglycans, except for one particular peptide (DPV1047) that partially originates from an anti-DNA antibody and is internalized in a caveolar independent manner. These new therapeutic tools are currently being developed for intracellular delivery of a number of active molecules and their potentiality for in vivo transduction investigated.

Therapeutic delivery using cell-penetrating peptides

2013 ◽  
Vol 5 (3) ◽  
Author(s):  
Rupa R. Sawant ◽  
Niravkumar R. Patel ◽  
Vladimir P. Torchilin
Keyword(s):  
Small Molecules ◽  
Therapeutic Agent ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptides ◽  
Current Status ◽  
Unique Challenge ◽  
Large Molecules ◽  
Therapeutic Delivery ◽  
Cell Penetrating ◽  
Stimuli Sensitive

AbstractIntracellular delivery of promising therapeutic agents as well as nanocarriers presents a unique challenge. However, with the discovery of the cell-penetrating peptides (CPPs), overcoming this obstacle seems more plausible. In many cases, CPPs conjugated with therapeutic agent or therapeutic agent loaded-nanoparticles have shown promising results via increased cellular uptake. In this review, the current status of CPPs for the intracellular delivery of not just potential therapeutic small molecules but also large molecules like peptides, nucleic acids and nanocarriers is discussed. In addition, the design of ‘smart stimuli-sensitive nanocarrier’ to overcome the non-target-specificity of CPPs is also described.

Intracellular Delivery of Histidine and Arginine Rich Cell Penetrating Peptides into HeLa cell Line

2015 ◽  
Vol 5 (4) ◽  
pp. 695
Author(s):  
Shikha Saxena ◽  
Deepika Bisht ◽  
Basavaraj Sajjanar ◽  
Arvind Kumar Singh ◽  
Aditya Prasad Sahoo ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptides ◽  
Hela Cell Line ◽  
Cell Penetrating
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