scholarly journals The Effect of Electrospun Fiber Diameter on Astrocyte-Mediated Neurite Guidance and Protection

2018 ◽  
Vol 2 (1) ◽  
pp. 104-117 ◽  
Author(s):  
Christopher D. L. Johnson ◽  
Jonathan M. Zuidema ◽  
Kathryn R. Kearns ◽  
Alianna B. Maguire ◽  
Gregory P. Desmond ◽  
...  
Keyword(s):  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Neurite Guidance

scholarly journals Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 643 ◽  
Author(s):  
Dae Geun Han ◽  
Chi Bum Ahn ◽  
Ji-Hyun Lee ◽  
Yongsung Hwang ◽  
Joo Hyun Kim ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Electrospun Nanofibers ◽  
Macrophage Infiltration ◽  
Vsmc Proliferation ◽  
Vascular Scaffolds ◽  
Poly Caprolactone

Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to determine an optimum electrospun fiber diameter for human vascular smooth muscle cell (VSMC) regeneration in vascular scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) electrospun fiber diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 μm, and VSMC survivals, proliferations, infiltrations, and phenotypes were recorded after culturing cells on these scaffolds for one, four, seven, or 10 days. VSMC phenotypes and macrophage infiltrations into scaffolds were evaluated by implanting scaffolds subcutaneously in a mouse for seven, 14, or 28 days. We found that human VSMC survival was not dependent on the electrospun fiber diameter. In summary, increasing fiber diameter reduced VSMC proliferation, increased VSMC infiltration and increased macrophage infiltration and activation. Our results indicate that electrospun PCL fiber diameters of 7 or 10 µm are optimum in terms of VSMC infiltration and macrophage infiltration and activation, albeit at the expense of VSMC proliferation.

Effect of Concentration on Electrospinning Oil Sorptive Fiber

2010 ◽  
Vol 160-162 ◽  
pp. 1611-1616
Author(s):  
Yan Ping Liu ◽  
Hua Bin Xu ◽  
Yi Bing Deng
Keyword(s):  
Fiber Diameter ◽  
Removal Rate ◽  
Electrospun Fiber ◽  
Scaling Law ◽  
Great Influence ◽  
Solution Concentration ◽  
Sorption Process ◽  
Processing Technique ◽  
Order Kinetic ◽  
Sorption Ability

Electrospinning is a convenient method used in the preparation of ultrafine oil sorptive fibers based on Ethylene-propylene-diene (EPDM), Electrospun fiber diameter, varying in large from micrometer to nanometer, mainly depends upon the solution concentration, which has great influence on this function fiber’s sorption ability. The non-linear relation between mean fiber diameter (d) and solution concentration (C) was investigated and it follows a simple scaling law in the form . This relationship can be used to predict the target fiber diameter and guide the processing technique. The effects of various C on oil sorption ability were investigated in a batch-sorption technique. A comparison of the kinetic models, showed that the removal rate fit the first order kinetic model well, which suggested the sorption process is rapid, can reach equilibrium within a short time.

Study of Electrospun fiber diameter using ANSOFT and ANSYS

2018 ◽  
Vol 5 (10) ◽  
pp. 21529-21537
Author(s):  
V. Mamtha ◽  
H.N. Narasimha Murthy ◽  
Pramod Authade ◽  
R. Sridhar
Keyword(s):  
Fiber Diameter ◽  
Electrospun Fiber

Morphology of Electrospun Natural Rubber with Acrylonitrile-Butadiene-Styrene

2009 ◽  
Vol 79-82 ◽  
pp. 1583-1586 ◽  
Author(s):  
Sarawuth Sithornkul ◽  
Poonsub Threepopnatkul
Keyword(s):  
Natural Rubber ◽  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Acrylonitrile Butadiene Styrene ◽  
Solution Concentration ◽  
Electrospun Membrane ◽  
Sem Images ◽  
Electrospinning Technique ◽  
Tetramethylthiuram Disulfide ◽  
Butadiene Styrene

This research was aimed to study the electrospinning of natural rubber (NR)-acrylonitrile butadiene styrene (ABS) blend. The NR used in this research was compounded in a torque rheometor, with stearic acid, wingstay-L, dibenzothiazoledisulfide (MBTS), tetramethylthiuram disulfide (TMTD) and sulfur, respectively. The compounded NR was blended with ABS by dissolving them in tetrahydrofuran (THF), concentration of solution included 5, 10, and 15 w/w%. The ratio of NR/ABS was varied by varying the content of ABS of 20, 30, 40 and 50 wt%. Then NR/ABS electrospun membrane was built up by electrospinning technique with high voltage 15 kV, flow rate 30 ml/h, collector distance 15, 20 and 25 cm and collected the electrospun fiber on rotating circular at 1000 rpm. The morphology of electrospun fibers were characterized by scanning electron microscope (SEM). SEM images showed that NR/ABS membranes had higher porosity with decreasing ABS contents. Decreasing ABS contents, decreasing solution concentration and increasing collector distance decreased fiber diameter of electrospun NR/ABS.

Role of Scaffold Architecture and Mechanical Properties of Electrospun Scaffolds in Cell Seeding

2009 ◽  
Vol 1239 ◽  
Author(s):  
Nandula D. Wanasekara ◽  
Ming Chen ◽  
Vijaya B Chalivendra ◽  
Sankha Bhowmick
Keyword(s):  
Mechanical Properties ◽  
Young’S Modulus ◽  
Fiber Diameter ◽  
Young's Modulus ◽  
Electrospun Fiber ◽  
Cell Movement ◽  
Cell Seeding ◽  
3T3 Fibroblasts ◽  
Spun Fiber ◽  
Lower Depth

AbstractSeeding a layer of cells at specific depths within scaffolds is an important optimization parameter for bi-layer skin models. The work presented investigated the effect of fiber diameter and its mechanical property on the depth of cell seeding for electro-spun fiber scaffold. Polycaprolactone (PCL) is used to generate scaffolds that are submicron (400nm) to micron (1100nm) using electro-spinning. 3T3 fibroblasts were seeded on the electro-spun fiber scaffold mat of 50-70 microns thickness in this study. In order to investigate the effect of fiber diameter on cell migration, first, the electrospun fiber scaffold was studied for variation of mechanical properties as a function of fiber diameters. Atomic force microscopy (AFM) was used to investigate the Young’s modulus (E) values as a function of fiber diameter. It was identified that as the fiber diameter increases, the Young’s modulus values decreases considerably from 1.1GPa to 200MPa. The variation in E is correlated with cell seeding depth as a function of vacuum pressure. A higher E value led to a lower depth of cell seeding (closer to the surface) indicating that nanofibrous scaffolds offer larger resistance to cell movement compared to microfibrous scaffolds.

Effect of Electrospun Fiber Diameter and Alignment on Macrophage Activation and Secretion of Proinflammatory Cytokines and Chemokines

2011 ◽  
Vol 12 (5) ◽  
pp. 1900-1911 ◽  
Author(s):  
Enrica Saino ◽  
Maria Letizia Focarete ◽  
Chiara Gualandi ◽  
Enzo Emanuele ◽  
Antonia I. Cornaglia ◽  
...  
Keyword(s):  
Proinflammatory Cytokines ◽  
Macrophage Activation ◽  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Cytokines And Chemokines

Development of Electrospun Shellac and Hydroxypropyl Cellulose Blended Nanofibers for Drug Carrier Application

2020 ◽  
Vol 859 ◽  
pp. 239-243
Author(s):  
Nawinda Chinatangkul ◽  
Sirikarn Pengon ◽  
Suchada Piriyaprasarth ◽  
Chutima Limmatvapirat ◽  
Sontaya Limmatvapirat
Keyword(s):  
Drug Delivery ◽  
Antimicrobial Agent ◽  
Flow Rate ◽  
Applied Voltage ◽  
Infusion Rate ◽  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Drug Carrier ◽  
Hydroxypropyl Cellulose ◽  
Electrospinning Process

The aim of this study was to develop the electrospun shellac (SHL) and hydroxypropyl cellulose (HPC) blended nanofibers for drug carrier application. The effects of polymer solution and electrospinning parameters, including SHL-HPC ratio, HPC concentration, applied voltage and flow rate, on the appearance of fibers were investigated. Based on the results, electrospun fiber was not obtained when a solution of HPC alone was employed. However, the fibers would be obviously fabricated as SHL was added to the HPC solution. An increase in the SHL ratio in SHL-HPC blended solution could accordingly lead to a remarkable enhance in the fiber diameter. In addition, the continuous nanofibers with less beads were gradually formulated when the HPC concentration was increased. The electrospinning parameters seemed to be significant. The elevation of infusion rate from 0.5 to 1 mL/h would contribute to the preparation of thick fibers with the diameters enlarging from 666.9 to 843.5 nm. With the applied voltage increasing from 15 to 30 kV during the electrospinning process, the fabrication of small nanofibers with the diameters reducing from 843.5 to 741.6 nm would be conducted. In this study, monolaurin (ML), a broad antimicrobial agent, was encapsulated into the SHL-HPC carrier for the purpose of drug delivery application. Regarding the result, the loaded concentration of ML could not be enhanced by introducing HPC to the SHL fibers.

Fabrication and Characterization of Polycaprolactone (PCL)/Gelatin Electrospun Fibers

2014 ◽  
Vol 554 ◽  
pp. 52-56 ◽  
Author(s):  
Mim Mim Lim ◽  
Naznin Sultana ◽  
Azli Bin Yahya
Keyword(s):  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Weight Ratio ◽  
Processing Parameters ◽  
Electrospun Fibers ◽  
Average Fiber Diameter ◽  
Polymer Blending ◽  
Electrospinning Technique ◽  
Sem Image

Over the past few decades, there has been considerable interest in developing electrospun fibers by using electrospinning technique for various applications. Polymer blending is one of the most effective methods in providing desired properties. In this study, synthetic polymer polycaprolactone (PCL) was blended together with natural polymer gelatin where both of them have different properties. It is done by using electrospinning technique. 10 %w/v and 14 %w/v PCL/gelatin electrospun fibers were successfully electrospun with different weight ratio. Processing parameters were set constant in this study and only solution parameters were altered. The optimized electrospun fiber formed was 14 %w/v PCL/gelatin 70:30 with average fiber diameter of 246.30 nm. No beaded fiber was formed in this scanning electron microscope (SEM) image. The result obtained also showed that by increasing the overall polymeric concentration of PCL/gelatin, average fiber diameter decreases. Fiber diameter was also found decreasing with the increase of the concentration of gelatin in the same concentratoin of PCL/gelatin blended electrospun fiber. Blending of PCL and gelatin in different weight ratio had provided different properties of electrospun fibers. It is believed that blended electrospun fibers can be used for biomedical applications.

Determination of electrospun fiber diameter distributions using image analysis processing

2008 ◽  
Vol 16 (4) ◽  
pp. 314-319 ◽  
Author(s):  
Eun Ho Shin ◽  
Kwang Soo Cho ◽  
Moon Hwo Seo ◽  
Hyungsup Kim
Keyword(s):  
Image Analysis ◽  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Diameter Distributions

Investigation of a New Needleless Electrospinning Method for the Production of Nanofibers

2013 ◽  
Vol 8 (4) ◽  
pp. 155892501300800 ◽  
Author(s):  
Deogratias Nurwaha ◽  
Wanli Han ◽  
Xinhou Wang
Keyword(s):  
Strong Interaction ◽  
Applied Voltage ◽  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Scaling Up ◽  
Processing Parameters ◽  
Needleless Electrospinning ◽  
Electrospinning Method ◽  
Multiple Jets ◽  
Productivity Rate

This paper presents the possibility of nanofiber formation by a new multiple jet method. A novel needleless electrospinning apparatus was used to produce nanofibers. This employs a new design for supplying solution to a metal roller spinneret. The advantage of this setup is its ease of scaling-up for increased output. Using this new method it was possible to increase the nanofiber production rate because of the multiple jets. The productivity rate has been significantly enhanced and was 24–30 times higher than single needle electrospinning. It was also possible to produce thinner fibers than the single needle method. It was found that fibers produced by this novel needleless electrospinning had fewer beadings than fibers produced by the conventional electrospinning method. The effects of processing parameters including applied voltage and spinning electrospinning distance on eletrospun fiber diameter were also investigated. The study showed that the electrospun fiber diameter was strongly governed by the processing parameters. It was observed that there was a strong interaction between these parameters.

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