High-Yield Method To Fabricate and Functionalize DNA Nanoparticles from the Products of Rolling Circle Amplification

2018 ◽  
Vol 1 (2) ◽  
pp. 511-519 ◽  
Author(s):  
Xuexia Yuan ◽  
Fan Xiao ◽  
Haoran Zhao ◽  
Yishun Huang ◽  
Chen Shao ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
High Yield ◽  
Rolling Circle ◽  
Dna Nanoparticles

Efficient Self‐Assembled DNA Nanoparticles through Rolling Circle Amplification for siRNA Delivery in v itro

2019 ◽  
Vol 37 (6) ◽  
pp. 588-592 ◽  
Author(s):  
Qian Yao ◽  
Yuqi Chen ◽  
Fan Wu ◽  
Fan Wu ◽  
Chaoxing Liu ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Sirna Delivery ◽  
Rolling Circle ◽  
Dna Nanoparticles ◽  
Self Assembled

scholarly journals Shaping Rolling Circle Amplification Products into DNA Nanoparticles by Incorporation of Modified Nucleotides and Their Application to In Vitro and In Vivo Delivery of a Photosensitizer

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1833 ◽  
Author(s):  
Kyoung-Ran Kim ◽  
Pascal Röthlisberger ◽  
Seong Kang ◽  
Kihwan Nam ◽  
Sangyoup Lee ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cellular Uptake ◽  
Rolling Circle Amplification ◽  
Modified Nucleotides ◽  
Rolling Circle ◽  
Dna Nanoparticles ◽  
Nuclease Resistance ◽  
Cellular Uptake Efficiency

Rolling circle amplification (RCA) is a robust way to generate DNA constructs, which are promising materials for biomedical applications including drug delivery because of their high biocompatibility. To be employed as a drug delivery platform, however, the DNA materials produced by RCA need to be shaped into nanoparticles that display both high cellular uptake efficiency and nuclease resistance. Here, we showed that the DNA nanoparticles (DNPs) can be prepared with RCA and modified nucleotides that have side-chains appended on the nucleobase are capable of interacting with the DNA strands of the resulting RCA products. The incorporation of the modified nucleotides improved cellular uptake efficiency and nuclease resistance of the DNPs. We also demonstrated that these DNPs could be employed as carriers for the delivery of a photosensitizer into cancer cells to achieve photodynamic therapy upon irradiation at both the in vitro and in vivo levels.

Biophysical and chemical handles to control the size of DNA nanoparticles produced by rolling circle amplification

2016 ◽  
Vol 4 (9) ◽  
pp. 1314-1317 ◽  
Author(s):  
So Yeon Lee ◽  
Kyoung-Ran Kim ◽  
Duhee Bang ◽  
Se Won Bae ◽  
Hak Joong Kim ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Modified Nucleotides ◽  
Rolling Circle ◽  
Dna Nanoparticles

The size of DNA nanoparticles produced by rolling circle amplification (RCA) can be controlled by incorporation of structure-forming sequences and amine-modified nucleotides in the replication part.

Size-controlled synthesis of polymerized DNA nanoparticles for targeted anticancer drug delivery

2019 ◽  
Vol 55 (34) ◽  
pp. 4905-4908 ◽  
Author(s):  
Keonwook Nam ◽  
Taehyung Kim ◽  
Young Min Kim ◽  
Kyungjik Yang ◽  
Deokyoung Choe ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Rolling Circle Amplification ◽  
Controlled Synthesis ◽  
Layer By Layer ◽  
Rolling Circle ◽  
Anticancer Drug Delivery ◽  
Dna Nanoparticles ◽  
Targeted Drug ◽  
Size Controlled

The size-tunable polymerized DNA nanoparticles (PDNs) for cancer-targeted drug delivery were synthesized via sequential processes of rolling circle amplification, condensation, and layer-by-layer assembly.

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

scholarly journals Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shan Shen ◽  
Shi-Liang Liu ◽  
Ji-Hang Jiang ◽  
Li-Wei Zhou
Keyword(s):  
Species Identification ◽  
Rolling Circle Amplification ◽  
Practical Method ◽  
Morphological Characters ◽  
Its Sequences ◽  
Commercial Development ◽  
Medicinal Mushrooms ◽  
Rolling Circle ◽  
Reference Sequences ◽  
Its Barcoding

Abstract“Sanghuang” refers to a group of important traditionally-used medicinal mushrooms belonging to the genus Sanghuangporus. In practice, species of Sanghuangporus referred to in medicinal studies and industry are now differentiated mainly by a BLAST search of GenBank with the ITS barcoding region as a query. However, inappropriately labeled ITS sequences of “Sanghuang” in GenBank restrict accurate species identification and, to some extent, the utilization of these species as medicinal resources. We examined all available 271 ITS sequences related to “Sanghuang” in GenBank including 31 newly submitted sequences from this study. Of these sequences, more than half were mislabeled so we have now corrected the corresponding species names. The mislabeled sequences mainly came from strains utilized by non-taxonomists. Based on the analyses of ITS sequences submitted by taxonomists as well as morphological characters, we separate the newly described Sanghuangporus subbaumii from S. baumii and treat S. toxicodendri as a later synonym of S. quercicola. Fourteen species of Sanghuangporus are accepted, with intraspecific distances up to 1.30% (except in S. vaninii, S. weirianus and S. zonatus) and interspecific distances above 1.30% (except between S. alpinus and S. lonicerinus, and S. baumii and S. subbaumii). To stabilize the concept of these 14 species of Sanghuangporus, their taxonomic information and reliable ITS reference sequences are provided. Moreover, ten potential diagnostic sequences are provided for Hyperbranched Rolling Circle Amplification to rapidly confirm three common commercial species, viz. S. baumii, S. sanghuang, and S. vaninii. Our results provide a practical method for ITS barcoding-based species identification of Sanghuangporus and will promote medicinal studies and commercial development from taxonomically correct material.

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