Elucidating Gold–MnO2 Core–Shell Nanoenvelope for Real Time SERS-Guided Photothermal Therapy on Pancreatic Cancer Cells

2021 ◽  
Author(s):  
Palasseri T. Sujai ◽  
Shanmughan Shamjith ◽  
Manu M. Joseph ◽  
Kaustabh Kumar Maiti
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Photothermal Therapy ◽  
Core Shell ◽  
Pancreatic Cancer Cells

In vitro evaluation of cytotoxic and apoptotic activities of ethanolic extract of sweet apricot kernel on PANC-1 pancreatic cancer cells

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Fatemeh Aamazadeh ◽  
Jaleh Barar ◽  
Yalda Rahbar Saadat ◽  
Alireza Ostadrahimi
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Ethanolic Extract ◽  
B Cell Lymphoma ◽  
Normal Cells ◽  
Content Type ◽  
Apricot Kernel ◽  
Pancreatic Cancer Cells ◽  
Apoptotic Effects

Purpose This study aimed to evaluate the cytotoxic/apoptotic effects of sweet apricot kernel ethanolic extract (SAEE) on human cancerous PANC-1 and 293/KDR normal cells. Design/methodology/approach The extract was prepared by maceration, and its chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). The biological effects of SAEE on PANC-1 and 293/KDR cells were investigated using MTT (3–(4, 5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide) assay, DAPI (4',6-diamidino-2-phenylindole) and AnnexinV/propidium iodide (PI) staining. The expression of pro- and anti-apoptotic genes was evaluated by real-time quantitative polymerase chain reaction (real-time q-PCR) analysis. Findings The SAEE showed the selective growth inhibitory activity against PANC-1 cells with an IC50 (the 50% inhibitory concentration) value of about 1 mg/mL at 72 h. Further investigations by DAPI staining and flow cytometry revealed nucleus fragmentation and elevation of apoptotic cells, respectively. Also, a significant decrease in B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated x protein (Bax) ratio (0.41, p = 0.001) and the up-regulation of caspase-3 expression (1.5 fold, p = 0.002) indicated the induction of apoptosis in PANC-1 cells but not in 293/KDR non-cancerous cells. These results suggest that SAEE could induce apoptosis in cancer cells via a mitochondrial dependent pathway. Furthermore, GC-MS analysis showed that the SAEE is rich in γ-sitosterol and γ-tocopherol. Overall, the findings suggest that because of the selective impacts of SAEE on PANC-1 cells, it can be considered as a supportive care in adjuvant therapy for pancreatic cancer. However, the potent anticancer effects of main components of SAEE and its clinical value as an antitumor drug should be further investigated. Research limitations/implications Considerable limitations of this study were that the related mechanisms of selective impacts of SAEE on cancerous and normal cells and potent cytotoxic/apoptotic effects of γ-sitosterol and γ-tocopherol as major components of SAEE were not investigated. Originality/value Recently, a growing interest has been dedicated to plant-based natural products. Sweet apricot kernel exerts a number of pharmacological activities; however, the anticancer effect, related mechanisms and its active compounds were rarely investigated. In this study, the authors aimed to evaluate the cytotoxic/apoptotic effects of SAEE on human cancerous PANC-1 and 293/KDR normal cells.

scholarly journals Investigation of Dextran-Coated Superparamagnetic Nanoparticles for Targeted Vinblastine Controlled Release, Delivery, Apoptosis Induction, and Gene Expression in Pancreatic Cancer Cells

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4721 ◽  
Author(s):  
Salim Albukhaty ◽  
Sharafaldin Al-Musawi ◽  
Salih Abdul Mahdi ◽  
Ghassan M. Sulaiman ◽  
Mona S. Alwahibi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Pancreatic Cancer ◽  
Transmission Electron Microscopy ◽  
Cancer Cells ◽  
Real Time ◽  
Superparamagnetic Nanoparticles ◽  
Apoptosis Induction ◽  
Pancreatic Cancer Cells ◽  
Transmission Electron ◽  
Biological Studies

In the current study, the surface of superparamagnetic iron oxide (SPION) was coated with dextran (DEX), and conjugated with folic acid (FA), to enhance the targeted delivery and uptake of vinblastine (VBL) in PANC-1 pancreatic cancer cells. Numerous analyses were performed to validate the prepared FA-DEX-VBL-SPION, such as field emission scanning transmission electron microscopy, high-resolution transmission electron microscopy, dynamic light scattering (DLS), Zeta Potential, Fourier transform infrared spectroscopy, and vibrating sample magnetometry (VSM). The delivery system capacity was evaluated by loading and release experiments. Moreover, in vitro biological studies, including a cytotoxicity study, cellular uptake assessment, apoptosis analysis, and real-time PCR, were carried out. The results revealed that the obtained nanocarrier was spherical with a suitable dispersion and without visible aggregation. Its average size, polydispersity, and zeta were 74 ± 13 nm, 0.080, and −45 mV, respectively. This dual functional nanocarrier also exhibited low cytotoxicity and a high apoptosis induction potential for successful VBL co-delivery. Real-time quantitative PCR analysis demonstrated the activation of caspase-3, NF-1, PDL-1, and H-ras inhibition, in PANC-1 cells treated with the FA-VBL-DEX-SPION nanostructure. Close inspection of the obtained data proved that the FA-VBL-DEX-SPION nanostructure possesses a noteworthy chemo-preventive effect on pancreatic cancer cells through the inhibition of cell proliferation and induction of apoptosis.

Ni–Au core–shell nanowires: synthesis, microstructures, biofunctionalization, and the toxicological effects on pancreatic cancer cells

2011 ◽  
Vol 21 (32) ◽  
pp. 12089 ◽  
Author(s):  
In Tak Jeon ◽  
Moon Kyu Cho ◽  
Jin Woo Cho ◽  
Boo Hyun An ◽  
Jun Hua Wu ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Core Shell ◽  
Toxicological Effects ◽  
Pancreatic Cancer Cells ◽  
Shell Nanowires

scholarly journals Designing Protease Sensors for Real-Time Imaging of Trypsin Activation in Pancreatic Cancer Cells†

Biochemistry ◽  
2009 ◽  
Vol 48 (15) ◽  
pp. 3519-3526 ◽  
Author(s):  
Ning Chen ◽  
Jin Zou ◽  
Siming Wang ◽  
Yiming Ye ◽  
Yun Huang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Real Time Imaging ◽  
Pancreatic Cancer Cells
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