Efficient Amyloid Fibrillation Inhibition and Remodeling of Preformed Fibrils of Bovine Insulin by Propolis Polyphenols-Based Nanosheets

2021 ◽  
Vol 4 (4) ◽  
pp. 3547-3560
Author(s):  
Mohammad Ramezani ◽  
Maryam Dehghan Hesami ◽  
Yasin Rafiei ◽  
Ebrahim Rostampour Ghareghozloo ◽  
Ali Akbar Meratan ◽  
...  
Keyword(s):  
Bovine Insulin ◽  
Amyloid Fibrillation

scholarly journals Role of surface oxygen-containing functional groups of graphene oxide quantum dots on amyloid fibrillation of two model proteins

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244296
Author(s):  
Ebrahim Rostampour Ghareghozloo ◽  
Mohsen Mahdavimehr ◽  
Ali Akbar Meratan ◽  
Nasser Nikfarjam ◽  
Atiyeh Ghasemi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Graphene Oxide ◽  
Nile Red ◽  
Structural Features ◽  
Bovine Insulin ◽  
Potential Measurement ◽  
Egg White Lysozyme ◽  
Amyloid Fibrillation ◽  
Graphene Oxide Quantum Dots

There are many reports demonstrating that various derivatives of carbon nanoparticles are effective inhibitors of protein aggregation. As surface structural features of nanoparticles play a key role on modulating amyloid fibrillation process, in the present in vitro study, bovine insulin and hen egg white lysozyme (HEWL) were selected as two model proteins to investigate the reducing effect of graphene oxide quantum dots (GOQDs) on their assembly under amyloidogenic conditions. GOQDs were prepared through direct pyrolysis of citric acid, and the reduction step was carried out using ascorbic acid. The prepared nanoparticles were characterized by UV-Vis, X-ray photoelectron, and FT-IR spectroscopies, transmission electron and atomic force microscopies, zeta potential measurement, and Nile red fluorescence assay. They showed the tendencies to modulate the assembly of the proteins through different mechanisms. While GOQDs appeared to have the capacity to inhibit fibrillation, the presence of reduced GOQDs (rGOQDs) was found to promote protein assembly via shortening the nucleation phase, as suggested by ThT fluorescence data. Moreover, the structures produced in the presence of GOQDs or rGOQDs were totally nontoxic. We suggest that surface properties of these particles may be part of the differences in their mechanism(s) of action.

Preparation of the [Ala16-B] analog of bovine insulin

1978 ◽  
Vol 14 (4) ◽  
pp. 472-473
Author(s):  
Yu. P. Shvachkin ◽  
E. N. Voluiskaya ◽  
V. F. Krivtsov ◽  
L. A. Petrova ◽  
V. V. Knyazeva ◽  
...  
Keyword(s):  
Bovine Insulin

Crystalline [A21-Desamido] Bovine Insulin

1978 ◽  
Vol 359 (2) ◽  
pp. 1229-1236 ◽  
Author(s):  
Barratt R. D. EASTER ◽  
Donald A. SUTTON ◽  
Siegfried E. DREWES
Keyword(s):  
Bovine Insulin
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