Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications

2021 ◽  
Vol 4 (2) ◽  
pp. 1900-1911
Author(s):  
Hun Hwi Cho ◽  
Su Young Been ◽  
Woo Youp Kim ◽  
Jeong Min Choi ◽  
Joo Hee Choi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Comparative Study ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Gellan Gum ◽  
Engineering Applications ◽  
Bone Particles ◽  
Demineralized Bone

scholarly journals Development of an angiogenesis-promoting microvesicle-alginate-polycaprolactone composite graft for bone tissue engineering applications

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2040 ◽  
Author(s):  
Hui Xie ◽  
Zhenxing Wang ◽  
Liming Zhang ◽  
Qian Lei ◽  
Aiqi Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Umbilical Vein ◽  
Composite Graft ◽  
Electron Microscopic ◽  
Engineering Applications

One of the major challenges of bone tissue engineering applications is to construct a fully vascularized implant that can adapt to hypoxic environments in vivo. The incorporation of proangiogenic factors into scaffolds is a widely accepted method of achieving this goal. Recently, the proangiogenic potential of mesenchymal stem cell-derived microvesicles (MSC-MVs) has been confirmed in several studies. In the present study, we incorporated MSC-MVs into alginate-polycaprolactone (PCL) constructs that had previously been developed for bone tissue engineering applications, with the aim of promoting angiogenesis and bone regeneration. MSC-MVs were first isolated from the supernatant of rat bone marrow-derived MSCs and characterized by scanning electron microscopic, confocal microscopic, and flow cytometric analyses. The proangiogenic potential of MSC-MVs was demonstrated by the stimulation of tube formation of human umbilical vein endothelial cellsin vitro. MSC-MVs and osteodifferentiated MSCs were then encapsulated with alginate and seeded onto porous three-dimensional printed PCL scaffolds. When combined with osteodifferentiated MSCs, the MV-alginate-PCL constructs enhanced vessel formation and tissue-engineered bone regeneration in a nude mouse subcutaneous bone formation model, as demonstrated by micro-computed tomographic, histological, and immunohistochemical analyses. This MV-alginate-PCL construct may offer a novel, proangiogenic, and cost-effective option for bone tissue engineering.

Hydrogel composite scaffolds with an attenuated immunogenicity component for bone tissue engineering applications

2021 ◽  
Author(s):  
Chenyuan Gao ◽  
Wan Ting Sow ◽  
Yingying Wang ◽  
Yili Wang ◽  
Dejun Yang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Composite Scaffolds ◽  
Engineering Applications ◽  
Hydrogel Composite

Xenogeneic bones are potential templates for bone regeneration.

scholarly journals A Proposed Fabrication Method of Novel PCL-GEL-HAp Nanocomposite Scaffolds for Bone Tissue Engineering Applications

2010 ◽  
Vol 19 (4) ◽  
pp. 096369351001900 ◽  
Author(s):  
A. Hamlekhan ◽  
M. Mozafari ◽  
N. Nezafati ◽  
M. Azami ◽  
H. Hadipour
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Test ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Fabrication Method ◽  
Engineering Applications ◽  
Poly Caprolactone

In this study, poly(∊-caprolactone) (PCL), gelatin (GEL) and nanocrystalline hydroxyapatite (HAp) was applied to fabricate novel PCL-GEL-HAp nanaocomposite scaffolds through a new fabrication method. With the aim of finding the best fabrication method, after testing different methods and solvents, the best method and solvents were found, and the nanocomposites were prepared through layer solvent casting combined with freeze-drying. Acetone and distillated water were used as the PCL and GEL solvents, respectively. The mechanical test showed that the increasing of the PCL weight through the scaffolds caused the improvement of the final nanocomposite mechanical behavior due to the increasing of the ultimate stress, stiffness and elastic modulus (8 MPa for 0% wt PCL to 23.5 MPa for 50% wt PCL). The biomineralization investigation of the scaffolds revealed the formation of bone-like apatite layers after immersion in simulated body fluid (SBF). In addition, the in vitro cytotoxity of the scaffolds using L929 mouse fibroblast cell line (ATCC) indicated no sign of toxicity. These results indicated that the fabricated scaffold possesses the prerequisites for bone tissue engineering applications.

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