Catalytically Active Bioseparation Resin Utilizing a Covalent Intermediate for Tagless Protein Purification

2020 ◽  
Vol 3 (12) ◽  
pp. 8911-8922
Author(s):  
Ben Evert ◽  
Ben Vezina ◽  
Bernd H. A. Rehm
Keyword(s):  
Protein Purification ◽  
Catalytically Active ◽  
Covalent Intermediate

scholarly journals Engineering DNA templated nonribosomal peptide synthesis

2020 ◽  
Author(s):  
Hsin-Mei Huang ◽  
Philipp Stephan ◽  
Hajo Kries
Keyword(s):  
Assembly Lines ◽  
Nonribosomal Peptide ◽  
Peptide Synthetases ◽  
Enzyme Cascade ◽  
Catalytically Active ◽  
Nonribosomal Peptide Synthesis ◽  
Dna Strand ◽  
Dna Template ◽  
Enzyme Cascades ◽  
Covalent Intermediate

AbstractNanocontainers or macromolecular scaffolds for artificial biocatalytic cascades facilitate sequential enzyme reactions but diffusive escape of intermediates limits rate enhancement. Nonribosomal peptide synthetases (NRPS) naturally form gigantic assembly lines and prevent escape by covalently tethering intermediates. Here, we have built DNA-templated NRPS (DT-NRPS) by adding zinc finger tags to split NRPS modules. The zinc fingers direct the NRPS modules to 9-bp binding sites on a DNA strand, where they form a catalytically active enzyme cascade. DT-NRPS outperform previously reported DNA templated enzyme cascades in terms of DNA acceleration which demonstrates that covalent intermediate channeling is possible along the DNA template. Attachment of assembly line enzymes to a DNA scaffold is a promising catalytic strategy for the sequence-controlled biosynthesis of nonribosomal peptides and other polymers.

Growth of Catalytically Active Nanostructures in the Nonequilibrium Epitaxy Regime

2015 ◽  
Vol 60 (6) ◽  
pp. 546-552
Author(s):  
V.M. Gorshkov ◽  
◽  
V.V. Kuzmenko
Keyword(s):  
Catalytically Active

Sewage sludge as a precursor of adsorbents/catalysts for environmental applications

2007 ◽  
Vol 2 (1) ◽  
Author(s):  
A. Ros ◽  
C. Canals-Batlle ◽  
M.A. Lillo-Ródenas ◽  
E. Fuente ◽  
M. A. Montes-Morán ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Room Temperature ◽  
Waste Water Treatment ◽  
Textural Properties ◽  
Active Species ◽  
Elemental Sulphur ◽  
Gaseous Emissions ◽  
Removal Experiments ◽  
Catalytically Active

This paper focuses on the valorisation of solid residues obtained from the thermal treatment of sewage sludge. In particular, sewage sludge samples were collected from two waste water treatment plants (WWTPs) with different sludge line basic operations. After drying, sludges were heated up to 700 °C in appropriate ovens under diluted air (gasification) and inert (pyrolysis) atmospheres. The solids obtained, as well as the dried (raw) sludges, were characterised to determine their textural properties and chemical composition, including the speciation of their inorganic fraction. All the materials under study were employed as adsorbents/catalysts in H2S removal experiments at room temperature. It was found that, depending on the particular sludge characteristics, outstanding results can be achieved both in terms of retention capacities and selectivity. Some of the solids outperform commercially available sorbents specially designed for gaseous emissions control. In these adsorbents/catalysts, H2S is selectively oxidised to elemental sulphur most likely due to the presence of inorganic, catalytically active species. The role of the carbon-enriched part on these solids is also remarked.

Affinity Tags for Protein Purification

2020 ◽  
Vol 21 (8) ◽  
pp. 821-830
Author(s):  
Vibhor Mishra
Keyword(s):  
Protein Purification ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Protein Domain ◽  
Affinity Tag ◽  
Small Peptides ◽  
Affinity Tags ◽  
C Terminus ◽  
Solubility Enhancers ◽  
As Solubility

The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affinity tags are discussed. The affinity tags have been classified into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affinity towards a chromatography resin. The protein/domain tags often perform double duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag removal strategies after purification are discussed.

Antimyotoxic Activity of Synthetic Peptides Derived from Bothrops atrox Snake Gamma Phospholipase A2 Inhibitor Selected by Virtual Screening

2019 ◽  
Vol 19 (22) ◽  
pp. 1952-1961 ◽  
Author(s):  
J.C. Sobrinho ◽  
A.F. Francisco ◽  
R. Simões-Silva ◽  
A.M. Kayano ◽  
J.J. Alfonso Ruiz Diaz ◽  
...  
Keyword(s):  
Amino Acids ◽  
Molecular Docking ◽  
Synthetic Peptides ◽  
Cell Damage ◽  
Neutralization Assay ◽  
Phospholipase Activity ◽  
Phospholipases A2 ◽  
Catalytically Active ◽  
Bothrops Atrox

Background: Several studies have aimed to identify molecules that inhibit the toxic actions of snake venom phospholipases A2 (PLA2s). Studies carried out with PLA2 inhibitors (PLIs) have been shown to be efficient in this assignment. Objective: This work aimed to analyze the interaction of peptides derived from Bothrops atrox PLIγ (atPLIγ) with a PLA2 and to evaluate the ability of these peptides to reduce phospholipase and myotoxic activities. Methods: Peptides were subjected to molecular docking with a homologous Lys49 PLA2 from B. atrox venom modeled by homology. Phospholipase activity neutralization assay was performed with BthTX-II and different ratios of the peptides. A catalytically active and an inactive PLA2 were purified from the B. atrox venom and used together in the in vitro myotoxic activity neutralization experiments with the peptides. Results: The peptides interacted with amino acids near the PLA2 hydrophobic channel and the loop that would be bound to calcium in Asp49 PLA2. They were able to reduce phospholipase activity and peptides DFCHNV and ATHEE reached the highest reduction levels, being these two peptides the best that also interacted in the in silico experiments. The peptides reduced the myotubes cell damage with a highlight for the DFCHNV peptide, which reduced by about 65%. It has been suggested that myotoxic activity reduction is related to the sites occupied in the PLA2 structure, which could corroborate the results observed in molecular docking. Conclusion: This study should contribute to the investigation of the potential of PLIs to inhibit the toxic effects of PLA2s.

Catalytically active coatings for steam reforming systems: synthesis and catalytic properties

2019 ◽  
pp. 96-105
Author(s):  
M. L. Shishkova ◽  
N. V. Yakovleva
Keyword(s):  
Catalytic Properties ◽  
Raw Materials ◽  
Cold Gas Dynamic Spraying ◽  
Hydrogen Fuel ◽  
Functional Coatings ◽  
Science And Engineering ◽  
Powder Mixtures ◽  
Conversion Of Methane ◽  
Catalytically Active ◽  
Metal Support

The paper considers science and engineering aspects of catalytically active compositions creation as regards immobilized catalysts for reforming hydrocarbon raw materials into hydrogen fuel. The authors investigate synthesis of catalytic powder mixtures and manufacturing of functional coatings by supersonic cold gas dynamic spraying. Research results in the field of creation of catalysts for steam conversion of methane to hydrogenous fuel on the metal support (Cr15Al15 tape support) are given. Composite powder mixtures (Ni–Al–Al(OH)3– Ca(OH)2–Mg(OH)2) were used as starting materials.

Catalytically Active Powder Compositions for Hazardous Emission Reduction Systems

2020 ◽  
Vol 11 (6) ◽  
pp. 1341-1347
Author(s):  
T. S. Vinogradova ◽  
E. L. Gyulikhandanov ◽  
I. V. Ulin ◽  
B. V. Farmakovsky ◽  
N. V. Yakovleva
Keyword(s):  
Emission Reduction ◽  
Catalytically Active
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