Long-Term and Clinically Relevant Full-Thickness Human Skin Equivalent for Psoriasis

2020 ◽  
Vol 3 (10) ◽  
pp. 6639-6647
Author(s):  
Smriti Singh ◽  
Yvonne Marquardt ◽  
Rahul Rimal ◽  
Akihiro Nishiguchi ◽  
Sebastian Huth ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Equivalent ◽  
Full Thickness ◽  
Human Skin Equivalent

Optimized construction of a full thickness human skin equivalent using 3D bioprinting and a PCL/collagen dermal scaffold

Bioprinting ◽  
2021 ◽  
Vol 21 ◽  
pp. e00123
Author(s):  
Srinivas Ramasamy ◽  
Pooya Davoodi ◽  
Sanjairaj Vijayavenkataraman ◽  
Jia Heng Teoh ◽  
Anbu Mozhi Thamizhchelvan ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Equivalent ◽  
3D Bioprinting ◽  
Full Thickness ◽  
Human Skin Equivalent

scholarly journals Development of a Full-Thickness Human Skin Equivalent In Vitro Model Derived from TERT-Immortalized Keratinocytes and Fibroblasts

2015 ◽  
Vol 21 (17-18) ◽  
pp. 2448-2459 ◽  
Author(s):  
Christianne M.A. Reijnders ◽  
Amanda van Lier ◽  
Sanne Roffel ◽  
Duco Kramer ◽  
Rik J. Scheper ◽  
...  
Keyword(s):  
Human Skin ◽  
In Vitro Model ◽  
Skin Equivalent ◽  
Full Thickness ◽  
Vitro Model ◽  
Human Skin Equivalent

scholarly journals Coenzyme Q10 Efficacy Test for Human Skin Equivalents Using a Pumpless Skin-On-A-Chip System

2020 ◽  
Vol 21 (22) ◽  
pp. 8475 ◽  
Author(s):  
Jisue Kim ◽  
Kyunghee Kim ◽  
Gun Yong Sung
Keyword(s):  
Human Skin ◽  
Coenzyme Q10 ◽  
Transforming Growth Factor ◽  
Skin Equivalent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Full Thickness ◽  
Contraction Rate ◽  
Gene And Protein Expression ◽  
External Tube ◽  
Human Skin Equivalent

A human skin equivalent (HSE) composed of the epidermis and dermis is cultured using a pumpless skin-on-a-chip system to supply cultures the desired flow rate using gravity flow without a pump or an external tube connection. Coenzyme Q10 efficacy is tested by adjusting its concentration, as it is known to have anti-aging and antioxidant effects in culture solutions. The relationship between the contraction rate of a full-thickness human skin equivalent and secreted transforming growth factor (TGF) β-1 is analyzed via enzyme-linked immunosorbent assay (ELISA). Following hematoxylin and eosin (H&E) staining, an image of the skin equivalent is analyzed to measure the epidermal layer’s thickness. The cell density and differentiation of the dermis layer are investigated. Gene and protein expression in the dermal and epidermal layers are quantitatively analyzed using quantitative real time polymerase chain reaction (qPCR) and immunohistochemical staining. As the coenzyme Q10 treatment concentration increased, the number of cells per unit area and the thickness of the epidermal layer increased, the expression level of filaggrin increased, and the contraction rate of full-thickness HSE was proportional to the amount of TGF β-1 secreted.

Desmosomal distribution in the epidermis of a non-contracting human skin equivalent

1992 ◽  
Vol 50 (1) ◽  
pp. 896-897
Author(s):  
L.X. Oakford ◽  
S.D. Dimitrijevich ◽  
R. Gracy
Keyword(s):  
Human Skin ◽  
Basal Layer ◽  
Skin Equivalent ◽  
Tissue Component ◽  
Intact Skin ◽  
Similar Sequence ◽  
Sequence Of Events ◽  
Suprabasal Keratinocytes ◽  
Human Skin Equivalent

In intact skin the epidermal layer is a dynamic tissue component which is maintained by a basal layer of mitotically active cells. The protective upper epidermis, the stratum corneum, is generated by differentiation of the suprabasal keratinocytes which eventually desquamate as anuclear comeocytes. A similar sequence of events is observed in vitro in the non-contracting human skin equivalent (HSE) which was developed in this lab (1). As a part of the definition process for this model of living skin we are examining its ultrastructural features. Since desmosomes are important in maintaining cell-cell interactions in stratified epithelia their distribution in HSE was examined.

Transformative Encounters and Uncanny Intimacies: Biotechnology and Contemporary Art – A Practitioner's Approach and Perspective

Somatechnics ◽  
2012 ◽  
Vol 2 (2) ◽  
pp. 263-283 ◽  
Author(s):  
Svenja J. Kratz
Keyword(s):  
Tissue Culture ◽  
Human Skin ◽  
Contemporary Art ◽  
Skin Equivalent ◽  
Biomedical Innovation ◽  
Equivalent Models ◽  
Science Projects ◽  
Insight Into ◽  
Human Skin Equivalent

Abstract: Presented from an ArtScience practitioner's perspective, this paper provides an overview of Svenja Kratz's experience working as an artist within the area of cell and tissue culture at QUT's Institute of Health and Biomedical Innovation (IHBI). Using The Absence of Alice, a multi-medium exhibition based on the experience of culturing cells, as a case study, the paper gives insight into the artist's approach to working across art and science and how ideas, processes, and languages from each discipline can intermesh and extend the possibilities of each system. The paper also provides an overview of her most recent artwork, The Human Skin Equivalent/Experience Project, which involves the creation of personal jewellery items incorporating human skin equivalent models grown from the artist's skin and participant cells. Referencing this project, and other contemporary bioart works, the value of ArtScience is discussed, focusing in particular on the way in which cross-art-science projects enable an alternative voice to enter into scientific dialogues and have the potential to yield outcomes valuable to both disciplines.

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