A Mussel-Inspired Extracellular Matrix-Mimicking Composite Scaffold for Diabetic Wound Healing

2020 ◽  
Vol 3 (7) ◽  
pp. 4052-4061 ◽  
Author(s):  
Yuqi Jiang ◽  
You Li ◽  
Jiankai Li ◽  
Yiming Han ◽  
Pengju Zhang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Composite Scaffold ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

Cross-linked antifouling polysaccharide hydrogel coating as extracellular matrix mimics for wound healing

2017 ◽  
Vol 5 (16) ◽  
pp. 2989-2999 ◽  
Author(s):  
Yuqin Tang ◽  
Xueqin Cai ◽  
Yingying Xiang ◽  
Yu Zhao ◽  
Xinge Zhang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Diabetic Wound ◽  
Natural Polysaccharide ◽  
Diabetic Wound Healing ◽  
Polysaccharide Hydrogel ◽  
Hydrogel Coating

Antifouling hydrogel coating based on natural polysaccharide could effectively promote diabetic wound healing.

scholarly journals A Novel Composite Hydrogel Composed of Formic Acid-Decellularized Pepsin-Soluble Extracellular Matrix Hydrogel and Sacchachitin Hydrogel as Wound Dressing to Synergistically Accelerate Diabetic Wound Healing

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 538
Author(s):  
Chien-Ming Hsieh ◽  
Weu Wang ◽  
Ying-Hsuan Chen ◽  
Pu-Sheng Wei ◽  
Yu-Hsuan Liu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Formic Acid ◽  
Cell Viability ◽  
Hair Follicles ◽  
Composite Hydrogel ◽  
Sweat Glands ◽  
Diabetic Wound ◽  
Porcine Skin ◽  
Diabetic Wound Healing

Extracellular matrix (ECM) hydrogel can create a favorable regenerative microenvironment and act as a promising dressing for accelerating the healing of diabetic wound. In this study, a simple and effective decellularization technique was developed and optimized to obtain acellular extracellular matrix (aECM) from porcine skin. It was found that decellularization at 30% formic acid for 72 h effectively decellularized porcine skin while retaining >75% collagen and ~37% GAG in the aECM with no presence of nuclei of cellular remnants. aECM hydrogel was fabricated by digesting aECM with pepsin in various acidic solutions (0.1 N HCl, glycolic acid (GA) and 2-pyrrolidone-5-carboxylic acid (PCA)) and then treated with a pH-controlled neutralization and temperature-controlled gelation procedure. Based on physical characterizations, including SDS-PAGE, rheological analysis and SEM analysis, aECMHCl hydrogels fabricated at 25 mg/mL in 0.1 N HCl were selected. Four polymeric ECM-mimic hydrogels, including sacchachitin (SC), hyaluronic acid (HA) and chitosan (CS) and three composite hydrogels of combining SC either with aECMHCl,25 (aECMHCl/SC), HA (HA/SC) or CS (SC/CS) were prepared and evaluated for WS-1 cell viability and wound-healing effectiveness. Cell viability study confirmed that no hydrogel dressings possessed any toxicity at all concentrations examined and ECMHCl, HA and ECMHCl/SC at higher concentrations (>0.05%) induced statistically significant proliferation. Diabetic wound healing study and histological examinations revealed that ECMHCl/SC hydrogel was observed to synergistically accelerate wound healing and ultimately stimulated the growth of hair follicles and sweat glands in the healing wound indicating the wound had healed as functional tissues. The results support the great potential of this newly produced ECMHCl/SC composite hydrogel for healing and regeneration of diabetic wounds.

scholarly journals Insulin-Containing Wound Dressing Promotes Diabetic Wound Healing Through Stabilizing HIF-1α

2020 ◽  
Vol 8 ◽  
Author(s):  
Peilang Yang ◽  
Di Wang ◽  
Yan Shi ◽  
Mingzhong Li ◽  
Min Gao ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Wound Dressing ◽  
Control Group ◽  
Unstable State ◽  
Diabetic Wound ◽  
Diabetic State ◽  
Matrix Deposition ◽  
Diabetic Wound Healing

HIF-1α is seen as a major regulator during wound healing and controls many wound healing processes, such as angiogenesis, extracellular deposition, and reepithelialization. A diabetic state plays a vicious effect on wound healing, and the destabilization of HIF-1α is a non-negligible factor. Insulin-loaded silk fibroin microparticles were prepared to release insulin by covering the wounds, and this material was proven to promote wound healing in both in vitro and in vivo studies. In this work, we found that this insulin-containing wound dressing could accelerate diabetic wound healing by promoting reepithelialization, angiogenesis, and extracellular matrix, especially collagen deposition. Meanwhile, HIF-1α was stable and accumulated in insulin-containing dressing to group wound cells, which was significantly unstable in the control group. In further studies, we showed that methylglyoxal (MGO), the main form of advanced glycation end products (AGEs), accumulated significantly and caused the destabilization of HIF-1α in the diabetic state. Insulin could alleviate the MGO-induced HIF-1α unstable state and promote HIF-1α target gene expression and its downstream biological effect such as angiogenesis and wound extracellular matrix deposition.

A highly bioactive bone extracellular matrix-biomimetic nanofibrous system with rapid angiogenesis promotes diabetic wound healing

2017 ◽  
Vol 5 (35) ◽  
pp. 7285-7296 ◽  
Author(s):  
Wendong Gao ◽  
Wanwan Jin ◽  
Yannan Li ◽  
Li Wan ◽  
Chenggui Wang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Diabetic Wound ◽  
Nanofibrous Scaffolds ◽  
Diabetic Wound Healing

BGN-containing CPB nanofibrous scaffolds with rapid angiogenesis promotes diabetic wound healing.

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