Photo and Reduction Dual-Responsive Hydrogel for Regulating Cell Adhesion and Cell Sheet Harvest

2020 ◽  
Vol 3 (4) ◽  
pp. 2410-2418
Author(s):  
Yuan Xue ◽  
Duo Liu ◽  
Chenxi Wang ◽  
Chunyan Bao ◽  
Xuebin Wang ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Sheet ◽  
Dual Responsive

scholarly journals Cell Sheet Translocation: Facile Cell Sheet Harvest and Translocation Mediated by a Thermally Expandable Hydrogel with Controlled Cell Adhesion (Adv. Healthcare Mater. 18/2016)

2016 ◽  
Vol 5 (18) ◽  
pp. 2305-2305 ◽  
Author(s):  
Yu Bin Lee ◽  
Young Min Shin ◽  
Eun Mi Kim ◽  
Jangsoo Lim ◽  
Joong-Yup Lee ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Sheet

The quantification of single cell adhesion on functionalized surfaces for cell sheet engineering

Biomaterials ◽  
2010 ◽  
Vol 31 (25) ◽  
pp. 6436-6443 ◽  
Author(s):  
G. Weder ◽  
O. Guillaume-Gentil ◽  
N. Matthey ◽  
F. Montagne ◽  
H. Heinzelmann ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Functionalized Surfaces

Terminally Functionalized Thermoresponsive Polymer Brushes for Simultaneously Promoting Cell Adhesion and Cell Sheet Harvest

2011 ◽  
Vol 13 (1) ◽  
pp. 253-260 ◽  
Author(s):  
Hironobu Takahashi ◽  
Naoki Matsuzaka ◽  
Masamichi Nakayama ◽  
Akihiko Kikuchi ◽  
Masayuki Yamato ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Polymer Brushes ◽  
Cell Sheet ◽  
Thermoresponsive Polymer

scholarly journals Cell-cell adhesion interface: rise of the lateral membrane

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 276 ◽  
Author(s):  
Vivian W. Tang
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Steady State ◽  
Epithelial Cell ◽  
Mechanical Stability ◽  
Cell Sheet ◽  
Cell Movement ◽  
Dynamic Processes ◽  
Lateral Membrane ◽  
Cell Extrusion

The lateral membrane plays an important role in the mechanical stability of epithelial cell sheet in steady state. In addition, the lateral membrane is continuously remodeled during dynamic processes such as cell extrusion, cytokinesis, and intercellular cell movement. In wound healing, the lateral membrane must be built from flat and spread cells that had crawled into the area of the wound. Thus, forming the lateral membrane is a phenomenon that occurs not only in development but also during homeostatic maintenance and regeneration of differentiated epithelial tissues.

Controlled release of cell sheet by saccharide and temperature dual-responsive hydrogel layer

2015 ◽  
Vol 213 ◽  
pp. e36-e37
Author(s):  
Bingbing Guo ◽  
Qianping Guo ◽  
Guoqing Pan ◽  
Bin Li ◽  
Huilin Yang
Keyword(s):  
Controlled Release ◽  
Cell Sheet ◽  
Dual Responsive

scholarly journals Epithelial Morphogenesis Driven by Cell-Matrix vs. Cell-Cell Adhesion

2020 ◽  
Author(s):  
Shaohe Wang ◽  
Kazue Matsumoto ◽  
Kenneth M. Yamada
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Cell Sheet ◽  
Branching Morphogenesis ◽  
Epithelial Morphogenesis ◽  
Epithelial Surface ◽  
Matrix Adhesion ◽  
Cell Matrix ◽  
Cell Matrix Adhesion ◽  
Cell Cell

SUMMARYMany embryonic organs undergo epithelial morphogenesis to form tree-like hierarchical structures. However, it remains unclear what drives the budding and branching of stratified epithelia, such as in embryonic salivary gland and pancreas. Here, we performed live-organ imaging of mouse embryonic salivary glands at single-cell resolution to reveal that budding morphogenesis is driven by expansion and folding of a distinct epithelial surface cell sheet characterized by strong cell-matrix adhesions and weak cell-cell adhesions. Profiling of single-cell transcriptomes of this epithelium revealed spatial patterns of transcription underlying these cell adhesion differences. We then synthetically reconstituted budding morphogenesis by experimentally suppressing E-cadherin expression and inducing basement membrane formation in 3D spheroid cultures of engineered cells, which required β1 integrin-mediated cell-matrix adhesion for successful budding. Thus, stratified epithelial budding, the key first step of branching morphogenesis, is driven by an overall combination of strong cell-matrix adhesion and weak cell-cell adhesion by peripheral epithelial cells.

scholarly journals Interplay between integrins and PI4P5K Sktl is crucial for cell polarization and reepithelialisation during Drosophila wound healing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Si-Hyoung Park ◽  
Chan-wool Lee ◽  
Kwang-Min Choe
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Cell Polarity ◽  
Myosin Ii ◽  
Cell Sheet ◽  
Cell Polarization ◽  
Actin Dynamics ◽  
Rear Side ◽  
Jnk Pathway ◽  
Pathway Activation

Abstract Phosphatidylinositol(4,5)-bisphosphate [PI(4,5)P2] regulates cell adhesion and actin dynamics during cell migration. PI(4,5)P2 binds various components of the cell adhesion machinery, but how these processes affect migration of the epithelial cell sheet is not well understood. Here, we report that PI(4,5)P2 and Sktl, the kinase that converts PI4P to PI(4,5)P2, are both localized to the rear side of cells during wound healing of the Drosophila larval epidermis. The Sktl localization requires JNK pathway activation and integrins, but not PVR. The sktl knockdown epidermis displays strong defects in would closure, reminiscent of the JNK-depleted epidermis, and shows severe disruption of cell polarity, as determined by myosin II localization. Sktl and βPS integrin colocalize at the rear side of cells forming the trailing edge during wound healing and the two are inter-dependent in that the absence of one severely disrupts the rear localization of the other. These results strongly suggest that the JNK pathway regulates the rear localization of Sktl and integrins and the interplay between Sktl and integrins sets up cell polarity, which is crucial for reepithelialisation during wound healing.

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