Temperature-sensitive chitosan membranes as a substrate for cell adhesion and cell sheet detachment

2011 ◽  
Vol 23 (3) ◽  
pp. 447-453 ◽  
Author(s):  
Pingping Nie ◽  
Xiaoling He ◽  
Li Chen
Keyword(s):  
Cell Adhesion ◽  
Cell Sheet ◽  
Temperature Sensitive ◽  
Chitosan Membranes

scholarly journals Cell Sheet Translocation: Facile Cell Sheet Harvest and Translocation Mediated by a Thermally Expandable Hydrogel with Controlled Cell Adhesion (Adv. Healthcare Mater. 18/2016)

2016 ◽  
Vol 5 (18) ◽  
pp. 2305-2305 ◽  
Author(s):  
Yu Bin Lee ◽  
Young Min Shin ◽  
Eun Mi Kim ◽  
Jangsoo Lim ◽  
Joong-Yup Lee ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Sheet

scholarly journals Utilizing temperature-sensitive association of Pluronic F-127 with lipid bilayers to control liposome–cell adhesion

2002 ◽  
Vol 1559 (1) ◽  
pp. 32-42 ◽  
Author(s):  
Parthapratim Chandaroy ◽  
Arindam Sen ◽  
Paschalis Alexandridis ◽  
Sek Wen Hui
Keyword(s):  
Cell Adhesion ◽  
Lipid Bilayers ◽  
Temperature Sensitive

The quantification of single cell adhesion on functionalized surfaces for cell sheet engineering

Biomaterials ◽  
2010 ◽  
Vol 31 (25) ◽  
pp. 6436-6443 ◽  
Author(s):  
G. Weder ◽  
O. Guillaume-Gentil ◽  
N. Matthey ◽  
F. Montagne ◽  
H. Heinzelmann ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Functionalized Surfaces

Terminally Functionalized Thermoresponsive Polymer Brushes for Simultaneously Promoting Cell Adhesion and Cell Sheet Harvest

2011 ◽  
Vol 13 (1) ◽  
pp. 253-260 ◽  
Author(s):  
Hironobu Takahashi ◽  
Naoki Matsuzaka ◽  
Masamichi Nakayama ◽  
Akihiko Kikuchi ◽  
Masayuki Yamato ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Polymer Brushes ◽  
Cell Sheet ◽  
Thermoresponsive Polymer

scholarly journals Cell-cell adhesion interface: rise of the lateral membrane

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 276 ◽  
Author(s):  
Vivian W. Tang
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Steady State ◽  
Epithelial Cell ◽  
Mechanical Stability ◽  
Cell Sheet ◽  
Cell Movement ◽  
Dynamic Processes ◽  
Lateral Membrane ◽  
Cell Extrusion

The lateral membrane plays an important role in the mechanical stability of epithelial cell sheet in steady state. In addition, the lateral membrane is continuously remodeled during dynamic processes such as cell extrusion, cytokinesis, and intercellular cell movement. In wound healing, the lateral membrane must be built from flat and spread cells that had crawled into the area of the wound. Thus, forming the lateral membrane is a phenomenon that occurs not only in development but also during homeostatic maintenance and regeneration of differentiated epithelial tissues.

scholarly journals V-src kinase shifts the cadherin-based cell adhesion from the strong to the weak state and beta catenin is not required for the shift.

1995 ◽  
Vol 131 (6) ◽  
pp. 1839-1847 ◽  
Author(s):  
H Takeda ◽  
A Nagafuchi ◽  
S Yonemura ◽  
S Tsukita ◽  
J Behrens ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Tyrosine Phosphorylation ◽  
Mdck Cells ◽  
Src Kinase ◽  
Cell Aggregation ◽  
Temperature Sensitive ◽  
Beta Catenin ◽  
Weak State ◽  
Adhesion Ability ◽  
Phosphorylation Level

The elevation of tyrosine phosphorylation level is thought to induce the dysfunction of cadherin through the tyrosine phosphorylation of beta catenin. We evaluated this assumption using two cell lines. First, using temperature-sensitive v-src-transfected MDCK cells, we analyzed the modulation of cadherin-based cell adhesion by tyrosine phosphorylation. Cell aggregation and dissociation assays at nonpermissive and permissive temperatures indicated that elevation of the tyrosine phosphorylation does not totally affect the cell adhesion ability of cadherin but shifts it from a strong to a weak state. The tyrosine phosphorylation levels of beta catenin, ZO-1, ERM (ezrin/radixin/moesin), but not alpha catenin, vinculin, and alpha-actinin, were elevated in the weak state. To evaluate the involvement of the tyrosine phosphorylation of beta catenin in this shift of cadherin-based cell adhesion, we introduced v-src kinase into L fibroblasts expressing the cadherin-alpha catenin fusion protein, in which beta catenin is not involved in cell adhesion. The introduction of v-src kinase in these cells shifted their adhesion from a strong to a weak state. These findings indicated that the tyrosine phosphorylation of beta catenin is not required for the strong-to-weak state shift of cadherin-based cell adhesion, but that the tyrosine phosphorylation of other junctional proteins, ERM, ZO-1 or unidentified proteins is involved.

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