Fluorescent Labeling Method Re-Evaluates the Intriguing Thermoresponsive Behavior of Poly(acrylamide-co-acrylonitrile)s with Upper Critical Solution Temperatures

2019 ◽  
Vol 52 (20) ◽  
pp. 7646-7660 ◽  
Author(s):  
Chie Otsuka ◽  
Yuko Wakahara ◽  
Kohki Okabe ◽  
Juri Sakata ◽  
Masaki Okuyama ◽  
...  
Keyword(s):  
Fluorescent Labeling ◽  
Labeling Method ◽  
Poly Acrylamide ◽  
Critical Solution Temperatures

scholarly journals A Novel Fluorescent Labeling Method Enables Monitoring of Spatio-Temporal Dynamics of Developing Microsporidia

2015 ◽  
Vol 63 (3) ◽  
pp. 318-325 ◽  
Author(s):  
Marianita Santiana ◽  
Peter M. Takvorian ◽  
Nihal Altan-Bonnet ◽  
Ann Cali
Keyword(s):  
Temporal Dynamics ◽  
Fluorescent Labeling ◽  
Spatio Temporal ◽  
Labeling Method

Dual Fluorescent Labeling Method to Visualize Plasmid DNA Degradation

2009 ◽  
Vol 20 (1) ◽  
pp. 163-169 ◽  
Author(s):  
Charudharshini Srinivasan ◽  
Shafiuddin Siddiqui ◽  
Lawrence K. Silbart ◽  
Fotios Papadimitrakopoulos ◽  
Diane J. Burgess
Keyword(s):  
Plasmid Dna ◽  
Dna Degradation ◽  
Fluorescent Labeling ◽  
Labeling Method

scholarly journals Development of a Simple Method for Labeling and Identification of Protein Binders in Art

Heritage ◽  
2019 ◽  
Vol 2 (3) ◽  
pp. 2444-2456
Author(s):  
Ooi ◽  
Salvador ◽  
Martins ◽  
Pereira ◽  
Caldeira ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Bovine Milk ◽  
Egg Yolk ◽  
Fluorescent Labeling ◽  
Cultural Value ◽  
Simple Method ◽  
Rabbit Skin ◽  
Hen’S Egg ◽  
Protein Binders ◽  
Labeling Method

Easel paintings are assets with an important historic and cultural value. They usually possess a multi-tiered structure, composed of different layers some of which may present protein binders, making it important to identify these materials for restoration and conservation purposes. We propose the identification of different protein binders by a new fluorescent labeling method employing a coumarin based chromophore, C392STP (sodium(E/Z)-4-(4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)benzoyl)-2,3,5,6-tetrafluorobenzenesulfo-nate). The method was optimized using commercial proteins and was further tested on proteins extracted from hen’s egg yolk, white bovine milk, and rabbit skin glue. To model more realistic conditions, paint models of easel paintings were prepared. The paint models were made with hen’s egg yolk, white bovine milk, and rabbit skin glue, mixed with different pigments and submitted to artificial aging. Then the extracted proteins from the paint models were labeled with C392 which allowed a sensitive and selective identification by polyacrylamide gel electrophoresis (PAGE) of the different protein binders used. As a final test, three 19th century easel paintings, from the Italian painter Giorgio Marini, were analyzed. The results show the potential of the proposed method for the identification of protein binders present in easel paintings.

scholarly journals Revealing the three-dimensional filler structure in a rubber matrix based on fluorescein modified layered double hydroxides

RSC Advances ◽  
2017 ◽  
Vol 7 (7) ◽  
pp. 4030-4038 ◽  
Author(s):  
Weiyang Lv ◽  
Miao Du ◽  
Jianliang Xiao ◽  
Yihu Song ◽  
Qiang Zheng
Keyword(s):  
Layered Double Hydroxides ◽  
Three Dimensional ◽  
Structural Evolution ◽  
Fluorescent Labeling ◽  
Rubber Matrix ◽  
Payne Effect ◽  
Double Hydroxides ◽  
Labeling Method ◽  
Filler Structure

A facile fluorescent labeling method was developed to track the filler structural evolution in a rubber matrix, which could be helpful in understanding the Payne effect in the filled compounds.

scholarly journals Optimization of Fluorescent Labeling for In Vivo Nanoimaging of Sarcomeres in the Mouse Heart

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Togo Shimozawa ◽  
Kotaro Oyama ◽  
Yasuharu Kushida ◽  
Takako Terui ◽  
...  
Keyword(s):  
Adenovirus Vector ◽  
Fluorescent Labeling ◽  
Fluorescence Labeling ◽  
Mouse Heart ◽  
Viral Particles ◽  
Tubular System ◽  
Health And Disease ◽  
T Tubules ◽  
Labeling Method

The present study was conducted to systematically investigate the optimal viral titer as well as the volume of the adenovirus vector (ADV) that expresses α-actinin-AcGFP in the Z-disks of myocytes in the left ventricle (LV) of mice. An injection of 10 μL ADV at viral titers of 2 to 4 × 1011 viral particles per mL (VP/mL) into the LV epicardial surface consistently expressed α-actinin-AcGFP in myocytes in vivo, with the fraction of AcGFP-expressing myocytes at ~10%. Our analysis revealed that SL was ~1.90-2.15 μm upon heart arrest via deep anesthesia. Likewise, we developed a novel fluorescence labeling method of the T-tubular system by treating the LV surface with CellMask Orange (CellMask). We found that the T-tubular distance was ~2.10-2.25 μm, similar to SL, in the healthy heart in vivo. Therefore, the present high-precision visualization method for the Z-disks or the T-tubules is beneficial to unveiling the mechanisms of myocyte contraction in health and disease in vivo.

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