Electrospinning Cargo-Containing Polyelectrolyte Complex Fibers: Correlating Molecular Interactions to Complex Coacervate Phase Behavior and Fiber Formation

2018 ◽  
Vol 51 (21) ◽  
pp. 8821-8832 ◽  
Author(s):  
Xiangxi Meng ◽  
Jessica D. Schiffman ◽  
Sarah L. Perry
Keyword(s):  
Phase Behavior ◽  
Molecular Interactions ◽  
Polyelectrolyte Complex ◽  
Fiber Formation ◽  
Complex Coacervate ◽  
Coacervate Phase

The Effects of Protein Charge Patterning on Complex Coacervation

2021 ◽  
Author(s):  
Nicholas Zervoudis ◽  
Allie Obermeyer
Keyword(s):  
Phase Boundary ◽  
Phase Behavior ◽  
Predictive Models ◽  
Phase Portraits ◽  
Complex Coacervation ◽  
Protein Encapsulation ◽  
Protein Charge ◽  
Polypeptide Sequence ◽  
The Impact ◽  
Coacervate Phase

The complex coacervation of proteins with other macromolecules has applications in protein encapsulation and delivery and for determining the function of cellular coacervates. Theoretical or empirical predictions for protein coacervates would enable the design of these coacervates with tunable and predictable structure-function relationships; unfortunately, no such theories exist. To help establish predictive models, the impact of protein-specific parameters on complex coacervation were probed in this study. The complex coacervation of sequence-specific, polypeptide-tagged, GFP variants and a strong synthetic polyelectrolyte was used to evaluate the effects of protein charge patterning on phase behavior. Phase portraits for the protein coacervates demonstrated that charge patterning dictates the protein’s binodal phase boundary. Protein concentrations over 100 mg mL<sup>-1</sup> were achieved in the coacervate phase, with concentrations dependent on the polypeptide sequence. In addition to shifting the binodal phase boundary, polypeptide charge patterning provided entropic advantages over isotropically patterned proteins. Together, these results show that modest changes of only a few amino acids alter the coacervation thermodynamics and can be used to tune the phase behavior of polypeptides or proteins of interest.

Molecular Interactions and Mixing Phase Behavior of Lipid Crystals

2018 ◽  
pp. 61-104 ◽  
Author(s):  
Eckhard Floeter ◽  
Michaela Haeupler ◽  
Kiyotaka Sato
Keyword(s):  
Phase Behavior ◽  
Molecular Interactions

scholarly journals Phase Behavior and Salt Partitioning in Polyelectrolyte Complex Coacervates

2018 ◽  
Vol 51 (8) ◽  
pp. 2988-2995 ◽  
Author(s):  
Lu Li ◽  
Samanvaya Srivastava ◽  
Marat Andreev ◽  
Amanda B. Marciel ◽  
Juan J. de Pablo ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Polyelectrolyte Complex

Charge-Density-Dominated Phase Behavior and Viscoelasticity of Polyelectrolyte Complex Coacervates

2019 ◽  
Vol 52 (13) ◽  
pp. 4957-4967 ◽  
Author(s):  
Jun Huang ◽  
Frances J. Morin ◽  
Jennifer E. Laaser
Keyword(s):  
Charge Density ◽  
Phase Behavior ◽  
Polyelectrolyte Complex

scholarly journals SANS study on the solvated structure and molecular interactions of a thermo-responsive polymer in a room temperature ionic liquid

2016 ◽  
Vol 18 (27) ◽  
pp. 17881-17889 ◽  
Author(s):  
Kazu Hirosawa ◽  
Kenta Fujii ◽  
Takeshi Ueki ◽  
Yuzo Kitazawa ◽  
Kenneth C. Littrell ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Phase Behavior ◽  
Molecular Interactions ◽  
Room Temperature ◽  
Room Temperature Ionic Liquid ◽  
Type Phase ◽  
Responsive Polymer

We have utilized SANS to quantitatively characterize the LCST-type phase behavior of PPhEtMA in d8-[C2mIm+][TFSA−].

Interfacial tension between a complex coacervate phase and its coexisting aqueous phase

Soft Matter ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 172-178 ◽  
Author(s):  
Evan Spruijt ◽  
Joris Sprakel ◽  
Martien A. Cohen Stuart ◽  
Jasper van der Gucht
Keyword(s):  
Interfacial Tension ◽  
Aqueous Phase ◽  
Complex Coacervate ◽  
Coacervate Phase

Rational design of food-grade polyelectrolyte complex coacervate for encapsulation and enhanced oral delivery of oenothein B

2017 ◽  
Vol 8 (11) ◽  
pp. 4070-4080 ◽  
Author(s):  
Yaqi Lan ◽  
Li Wang ◽  
Sufang Cao ◽  
Yinger Zhong ◽  
Yunqi Li ◽  
...  
Keyword(s):  
Controlled Release ◽  
Oral Delivery ◽  
Rational Design ◽  
Polyelectrolyte Complex ◽  
Gi Tract ◽  
Food Grade ◽  
Complex Coacervate ◽  
Oenothein B

Controlled release of OeB through GI tract using CPP–CS nanoparticles cross-linked with genipin was achievable.

scholarly journals Preparation of Poly(acrylate)/Poly(diallyldimethylammonium) Coacervates without Small Counterions and Their Phase Behavior upon Salt Addition towards Poly-Ions Segregation

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2259
Author(s):  
Marcos Vinícius Aquino Queirós ◽  
Watson Loh
Keyword(s):  
Phase Behavior ◽  
Polyelectrolyte Complex ◽  
Inorganic Salts ◽  
Salt Addition ◽  
Segregation Phenomenon ◽  
Strong Segregation ◽  
Polymer Surfactant ◽  
Oppositely Charged ◽  
Segregative Phase Separation ◽  
Hofmann Elimination

In this work, we report the phase behavior of polyelectrolyte complex coacervates (PECs) of poly(acrylate) (PA−) and poly(diallyldimethylammonium) (PDADMA+) in the presence of inorganic salts. Titrations of the polyelectrolytes in their acidic and alkaline forms were performed to obtain the coacervates in the absence of their small counterions. This approach was previously applied to the preparation of polymer–surfactant complexes, and we demonstrate that it also succeeded in producing complexes free of small counterions with a low extent of Hofmann elimination. For phase behavior studies, two different molar masses of poly(acrylate) and two different salts were employed over a wide concentration range. It was possible to define the regions at which associative and segregative phase separation take place. The latter one was exploited in more details because the segregation phenomenon in mixtures of oppositely charged polyelectrolytes is scarcely reported. Phase composition analyses showed that there is a strong segregation for both PA− and PDADMA+, who are accompanied by their small counterions. These results demonstrate that the occurrence of poly-ion segregation in these mixtures depends on the anion involved: in this case, it was observed with NaCl, but not with Na2SO4.

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