Stiffness Gradients in Glassy Polymer Model Nanocomposites: Comparisons of Quantitative Characterization by Fluorescence Spectroscopy and Atomic Force Microscopy

2017 ◽  
Vol 50 (14) ◽  
pp. 5447-5458 ◽  
Author(s):  
Min Zhang ◽  
Shadid Askar ◽  
John M. Torkelson ◽  
L. Catherine Brinson
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Spectroscopy ◽  
Glassy Polymer ◽  
Polymer Model ◽  
Quantitative Characterization ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Study of a Model Humic Acid-type Polymer by Fluorescence Spectroscopy and Atomic Force Microscopy

2012 ◽  
Vol 03 (07) ◽  
pp. 478-484
Author(s):  
Marcilene Ferrari Barriquello ◽  
Fábio de Lima Leite ◽  
Daiana Kotra Deda ◽  
Sérgio da Costa Saab ◽  
Nelson Consolin-Filho ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Humic Acid ◽  
Fluorescence Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force ◽  
Acid Type

scholarly journals DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 955
Author(s):  
Mark Freeley ◽  
Rebecca E. A. Gwyther ◽  
D. Dafydd Jones ◽  
Matteo Palma
Keyword(s):  
Atomic Force Microscopy ◽  
Carbon Nanotube ◽  
Fluorescence Spectroscopy ◽  
Emission Intensity ◽  
Directed Assembly ◽  
Electronic Coupling ◽  
Force Microscopy ◽  
Controlled Assembly ◽  
Atomic Force ◽  
Microscopy Images

Here, we report the controlled assembly of SWCNT–GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFPDNA conjugate. Atomic force microscopy images confirmed that GFP attachment occurred predominantly at the terminal ends of the nanotubes, as designed. The electronic coupling of the proteins to the nanotubes was confirmed via in-solution fluorescence spectroscopy, that revealed an increase in the emission intensity of GFP when linked to the CNTs.

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