Proteomic Analysis of Restored Insulin Production and Trafficking in Obese Diabetic Mouse Pancreatic Islets Following Euglycemia

2019 ◽  
Vol 18 (9) ◽  
pp. 3245-3258 ◽  
Author(s):  
Taewook Kang ◽  
Brandon B. Boland ◽  
Cristina Alarcon ◽  
Joseph S. Grimsby ◽  
Christopher J. Rhodes ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Proteomic Analysis ◽  
Diabetic Mouse ◽  
Insulin Production ◽  
Mouse Pancreatic Islets

scholarly journals Opposite effects of starvation on oxidation of [14C]adenosine and adenosine-induced insulin release by isolated mouse pancreatic islets

1978 ◽  
Vol 176 (2) ◽  
pp. 619-621 ◽  
Author(s):  
A Andersson
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Insulin Release ◽  
Metabolic Flux ◽  
Insulin Biosynthesis ◽  
Insulin Production ◽  
Stimulate Insulin Secretion ◽  
Mouse Pancreatic Islets

To test further the hypothesis that ribonucleosides stimulate insulin secretion and biosynthesis by producing metabolic signals, the effects of starvation on adenosine-stimulated insulin production and the oxidation of adenosine by isolated mouse pancreatic islets were examined. No direct correlation was found between the metabolic flux and insulin secretion, since the starvation-induced impairment of the adenosine-stimulated insulin secretion was accompanied by an increased rate of adenosine oxidation. Adenosine-stimulated insulin biosynthesis was, however, unaffected by starvation.

scholarly journals Dynamics of ADAM17-Mediated Shedding of ACE2 Applied to Pancreatic Islets of Male db/db Mice

Endocrinology ◽  
2015 ◽  
Vol 156 (12) ◽  
pp. 4411-4425 ◽  
Author(s):  
Kim Brint Pedersen ◽  
Harshita Chodavarapu ◽  
Constance Porretta ◽  
Leonie K. Robinson ◽  
Eric Lazartigues
Keyword(s):  
Pancreatic Islets ◽  
Diabetic Mouse ◽  
Activity Levels ◽  
Angiotensin Converting Enzyme 2 ◽  
Wide Range ◽  
Major Cell Type ◽  
Mouse Pancreatic Islets ◽  
Diabetes Progression ◽  
A Disintegrin And Metalloproteinase ◽  
Insulinoma Cells

Angiotensin-converting enzyme 2 (ACE2) gene therapy aimed at counteracting pancreatic ACE2 depletion improves glucose regulation in two diabetic mouse models: db/db mice and angiotensin II-infused mice. A disintegrin and metalloproteinase 17 (ADAM17) can cause shedding of ACE2 from the cell membrane. The aim of our studies was to determine whether ADAM17 depletes ACE2 levels in pancreatic islets and β-cells. Dynamics of ADAM17-mediated ACE2 shedding were investigated in 832/13 insulinoma cells. Within a wide range of ACE2 expression levels, including the level observed in mouse pancreatic islets, overexpression of ADAM17 increases shed ACE2 and decreases cellular ACE2 levels. We provide a mathematical description of shed and cellular ACE2 activities as a function of the ADAM17 activity. The effect of ADAM17 on the cellular ACE2 content was relatively modest with an absolute control strength value less than 0.25 and approaching 0 at low ADAM17 activities. Although we found that ADAM17 and ACE2 are both expressed in pancreatic islets, the β-cell is not the major cell type expressing ACE2 in islets. During diabetes progression in 8-, 12-, and 15-week-old db/db mice, ACE2 mRNA and ACE2 activity levels in pancreatic islets were not decreased over time nor significantly decreased compared with nondiabetic db/m mice. Levels of ADAM17 mRNA and ADAM17 activity were also not significantly changed. Inhibiting basal ADAM17 activity in mouse islets failed to affect ACE2 levels. We conclude that whereas ADAM17 has the ability to shed ACE2, ADAM17 does not deplete ACE2 from pancreatic islets in diabetic db/db mice.

Preferential Reduction of Insulin Production in Mouse Pancreatic Islets Maintained in Culture after Streptozotocin Exposure*

Endocrinology ◽  
1988 ◽  
Vol 122 (4) ◽  
pp. 1242-1249 ◽  
Author(s):  
DÉCIO L. EIZIRIK ◽  
STELLAN SANDLER ◽  
NILS WELSH ◽  
CLAES HELLERSTRÖM
Keyword(s):  
Pancreatic Islets ◽  
Insulin Production ◽  
Mouse Pancreatic Islets

Insulin production and glucose metabolism in isolated pancreatic islets of rats with NIDDM

Diabetes ◽  
1988 ◽  
Vol 37 (9) ◽  
pp. 1226-1233 ◽  
Author(s):  
B. Portha ◽  
M. H. Giroix ◽  
P. Serradas ◽  
N. Welsh ◽  
C. Hellerstrom ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Pancreatic Islets ◽  
Insulin Production ◽  
Isolated Pancreatic Islets

Slow [Ca2+]i oscillations induced by ketoisocaproate in single mouse pancreatic islets

Diabetes ◽  
1995 ◽  
Vol 44 (3) ◽  
pp. 300-305 ◽  
Author(s):  
F. Martin ◽  
J. V. Sanchez-Andres ◽  
B. Soria
Keyword(s):  
Pancreatic Islets ◽  
Mouse Pancreatic Islets
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