scholarly journals Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets

Diabetologia ◽  
1978 ◽  
Vol 14 (6) ◽  
pp. 397-404 ◽  
Author(s):  
A. Andersson
Keyword(s):  
Pancreatic Islets ◽  
Culture Media ◽  
Insulin Production ◽  
Mouse Pancreatic Islets ◽  
Culture Effects

scholarly journals Protection of the pancreatic β-cell glucoreceptor mechanism for insulin secretion during culture in chemically defined medium

1978 ◽  
Vol 174 (3) ◽  
pp. 959-964 ◽  
Author(s):  
Erik Gylfe
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Culture Media ◽  
Early Stage ◽  
Insulin Response ◽  
Defined Medium ◽  
Β Cell ◽  
Before And After ◽  
Mouse Pancreatic Islets ◽  
High Concentrations

High concentrations of glucose have a protective effect on the glucoreceptor mechanism for insulin secretion during culture of pancreatic islets in chemically defined media. To study at what level glucose exerts this effect, insulin secretion from β-cell-rich mouse pancreatic islets was measured before and after culture for 1 week in the presence of different substances. Before culture, glucose and inosine were potent stimulators, mannose and fructose were less potent and xylitol had no effect on secretion. Culture in 3mm-glucose resulted in a 10-fold decrease in the insulin response to glucose stimulation. A less marked decrease was noted after culture in 20mm- or 30mm-glucose. Inosine-stimulated secretion was much decreased after culture in high concentrations of glucose, whereas the responses to mannose or fructose were unchanged. After culture in 30mm-mannose, glucose-stimulated secretion was similar to that observed after culture in high concentrations of glucose, whereas the response to mannose had much decreased. There were no secretory responses to glucose or fructose after culture in 30mm-fructose, or to glucose or xylitol after culture in 30mm-xylitol. Culture in 10mm-inosine did not preserve any significant response to glucose or inosine. The insulin contents of islets and culture media were higher after culture in high concentrations of glucose, mannose or inosine than after culture in fructose, xylitol or low concentrations of glucose. It is suggested that glucose, and to some extent mannose, preserves the glucoreceptor mechanism for insulin secretion by influencing an early stage in glucose metabolism, presumably glucokinase activity.

scholarly journals Opposite effects of starvation on oxidation of [14C]adenosine and adenosine-induced insulin release by isolated mouse pancreatic islets

1978 ◽  
Vol 176 (2) ◽  
pp. 619-621 ◽  
Author(s):  
A Andersson
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Insulin Release ◽  
Metabolic Flux ◽  
Insulin Biosynthesis ◽  
Insulin Production ◽  
Stimulate Insulin Secretion ◽  
Mouse Pancreatic Islets

To test further the hypothesis that ribonucleosides stimulate insulin secretion and biosynthesis by producing metabolic signals, the effects of starvation on adenosine-stimulated insulin production and the oxidation of adenosine by isolated mouse pancreatic islets were examined. No direct correlation was found between the metabolic flux and insulin secretion, since the starvation-induced impairment of the adenosine-stimulated insulin secretion was accompanied by an increased rate of adenosine oxidation. Adenosine-stimulated insulin biosynthesis was, however, unaffected by starvation.

Proteomic Analysis of Restored Insulin Production and Trafficking in Obese Diabetic Mouse Pancreatic Islets Following Euglycemia

2019 ◽  
Vol 18 (9) ◽  
pp. 3245-3258 ◽  
Author(s):  
Taewook Kang ◽  
Brandon B. Boland ◽  
Cristina Alarcon ◽  
Joseph S. Grimsby ◽  
Christopher J. Rhodes ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Proteomic Analysis ◽  
Diabetic Mouse ◽  
Insulin Production ◽  
Mouse Pancreatic Islets

Preferential Reduction of Insulin Production in Mouse Pancreatic Islets Maintained in Culture after Streptozotocin Exposure*

Endocrinology ◽  
1988 ◽  
Vol 122 (4) ◽  
pp. 1242-1249 ◽  
Author(s):  
DÉCIO L. EIZIRIK ◽  
STELLAN SANDLER ◽  
NILS WELSH ◽  
CLAES HELLERSTRÖM
Keyword(s):  
Pancreatic Islets ◽  
Insulin Production ◽  
Mouse Pancreatic Islets

Insulin production and glucose metabolism in isolated pancreatic islets of rats with NIDDM

Diabetes ◽  
1988 ◽  
Vol 37 (9) ◽  
pp. 1226-1233 ◽  
Author(s):  
B. Portha ◽  
M. H. Giroix ◽  
P. Serradas ◽  
N. Welsh ◽  
C. Hellerstrom ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Pancreatic Islets ◽  
Insulin Production ◽  
Isolated Pancreatic Islets

Slow [Ca2+]i oscillations induced by ketoisocaproate in single mouse pancreatic islets

Diabetes ◽  
1995 ◽  
Vol 44 (3) ◽  
pp. 300-305 ◽  
Author(s):  
F. Martin ◽  
J. V. Sanchez-Andres ◽  
B. Soria
Keyword(s):  
Pancreatic Islets ◽  
Mouse Pancreatic Islets
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