Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar

2015 ◽  
Vol 14 (8) ◽  
pp. 3188-3203 ◽  
Author(s):  
Mélanie Mauriat ◽  
Jean-Charles Leplé ◽  
Stéphane Claverol ◽  
Jérôme Bartholomé ◽  
Luc Negroni ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Tension Wood ◽  
Wood Formation

ROLES OF AUXIN AND GIBBERELLIN IN GRAVITY-INDUCED TENSION WOOD FORMATION IN AESCULUS TURBINATA SEEDLINGS

IAWA Journal ◽  
2004 ◽  
Vol 25 (3) ◽  
pp. 337-347 ◽  
Author(s):  
Sheng Du ◽  
Hiroki Uno ◽  
Fukuju Yamamoto
Keyword(s):  
Tension Wood ◽  
Auxin Transport ◽  
Histological Analysis ◽  
Synergistic Effects ◽  
Wood Formation ◽  
Wood Fibers ◽  
Lower Side ◽  
Inhibitory Effects ◽  
Auxin Action ◽  
Ga Biosynthesis

The lowest nodes of 6-week-old Aesculus turbinata seedlings were treated with uniconazole-P, an inhibitor of gibberellin (GA) biosynthesis, or a mixture of uniconazole-P and GA3 in acetone solution. To the seedling stems, an inhibitor of auxin transport (NPA) or inhibitors of auxin action (raphanusanin or MBOA) were applied in lanolin paste. The seedlings were tilted at a 45° angle and kept for 10 weeks before histological analysis. Decreases in both normal and tension wood formation followed the application of uniconazole-P. The application of GA3 together with uniconazole-P partially negated the effect of uniconazole-P alone. The application of NPA inhibited tension wood formation at, above, and below the lanolin-treated portions. The treatment of raphanusanin or MBOA also resulted in decreases in tension wood formation at the treated portions. The inhibitory effects of these chemicals applied on the upper side of tilted stems or around the entire stem were greater than on the lower side. The application of uniconazole-P in combination with raphanusanin, MBOA or NPA showed synergistic effects on the inhibition of tension wood formation. The results suggest that both auxin and GA regulate the quantitative production of tension wood fibers and are essential to tension wood formation.

Ethylene Evolution Changes in TiltedFraxinus mandshuricaRupr. var.japonicaMaxim. Seedlings in Relation to Tension Wood Formation

2009 ◽  
Vol 51 (7) ◽  
pp. 707-713 ◽  
Author(s):  
Sha Jiang ◽  
Ke Xu ◽  
Na Zhao ◽  
Shu-Xin Zheng ◽  
Yan-Ping Ren ◽  
...  
Keyword(s):  
Tension Wood ◽  
Wood Formation ◽  
Ethylene Evolution

scholarly journals PtiCYP85A3, a BR C-6 Oxidase Gene, Plays a Critical Role in Brassinosteroid-Mediated Tension Wood Formation in Poplar

2020 ◽  
Vol 11 ◽  
Author(s):  
Yanli Jin ◽  
Chunyan Yu ◽  
Chunmei Jiang ◽  
Xiaotong Guo ◽  
Bei Li ◽  
...  
Keyword(s):  
Tension Wood ◽  
Critical Role ◽  
Wood Formation ◽  
Oxidase Gene

scholarly journals RNA-Seq analysis of differential gene expression in Betula luminifera xylem during the early stages of tension wood formation

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5427 ◽  
Author(s):  
Miaomiao Cai ◽  
Huahong Huang ◽  
Fei Ni ◽  
Zaikang Tong ◽  
Erpei Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tension Wood ◽  
Molecular Mechanisms ◽  
Southern China ◽  
Lignin Biosynthesis ◽  
Wood Formation ◽  
Cellulose Synthesis ◽  
Early Stages ◽  
Transcriptional Changes ◽  
Betula Luminifera

Background Betula luminifera H. Winkler, which is widely distributed in southern China, is an economically important broadleaf tree species. However, little genomic information of B. luminifera is available, and little is known about the molecular mechanisms of wood formation in this species. Meanwhile, few efforts have focused on investigating the early transcriptional changes during tension wood formation in woody plants. Results A reference transcriptome dataset was first generated containing 45,700 Unigenes, and 35,135 (76.9%) Unigenes were annotated by a BLAST similarity search against four public databases. Then, based on an anatomical investigation, the global gene expression changes during the early stages of tension wood formation were analyzed. Gene expression profiling showed that a total of 13,273 Unigenes were differentially regulated during the early stages of tension wood formation. Most genes involved in cellulose and lignin biosynthesis were highlighted to reveal their biological importance in tension wood formation. In addition, the transcription levels of many genes involved in the auxin response pathway were significantly changed during the early stages of tension wood formation. Furthermore, 18 TFs co-expressed with key enzymes of cellulose synthesis were identified. Conclusions Our results revealed the transcriptional changes associated with TW formation and identified potential key genes in the regulation of this process. These results will help to dissect the molecular mechanism of wood formation and provide key candidate genes for marker-assisted selection in B. luminifera.

Effect of exogenous IAA on tension wood formation by facilitating polar auxin transport and cellulose biosynthesis in hybrid poplar (Populus deltoids × Populus nigra) wood

Holzforschung ◽  
2017 ◽  
Vol 71 (2) ◽  
pp. 179-188 ◽  
Author(s):  
Min Yu ◽  
Kai Liu ◽  
Shengquan Liu ◽  
Haiyan Chen ◽  
Liang Zhou ◽  
...  
Keyword(s):  
Tension Wood ◽  
Auxin Transport ◽  
Hybrid Poplar ◽  
Wood Formation ◽  
Polar Auxin Transport ◽  
Fiber Formation ◽  
Pcr Analysis ◽  
Cellulose Content ◽  
Cellulose Biosynthesis ◽  
Exogenous Iaa

Abstract Auxins as phytohormons, responsible for coordination of growing processes, also contribute to the formation of tension wood (TW) in trees, but the mechanism of this process is still unclear. In this study, it has been tried to induce TW formation in erect hybrid poplar trees (in opposite to inclined or bended trees) by treatment with exogenous indole-3-acetic acid (IAA, as one of the auxins) or N-1-naphthylphthalamic acid (NPA) as an auxin transport inhibitor. The treatment with IAA resulted in TW formation and, as expected, NPA treatment did not. The gelatinous fiber formation and higher cellulose content in the cell wall were unambiguous indicators for TW formation. Real-time polymerase chain reaction (PCR) analysis revealed that genes of PIN1, ABCB1, and AUX2 involved in polar auxin transport were highly expressed in trees treated with exogenous IAA. Moreover, expressions of cellulose biosynthesis related genes of UGP1, UGP2 and CesA13 were strongly up-regulated. These observations indicate that the accelerated intercellular polar auxin transport caused by exogenous IAA is accounted for TW formation, i.e. the signal transduction of auxin is affected, which then facilitates cellulose biosynthesis. In contrast, the transcript abundances of PIN1 and all selected cellulose synthases (CesAs) were decreased after NPA treatment via inhibiting the cellular auxin efflux with negative effects on plant’s primary growth. These results are interpreted that TW formation is closely associated with the acceleration of intercellular polar auxin transport.

scholarly journals Protein expression in tension wood formation monitored at high tissue resolution in Populus

2017 ◽  
Vol 68 (13) ◽  
pp. 3405-3417 ◽  
Author(s):  
Joakim Bygdell ◽  
Vaibhav Srivastava ◽  
Ogonna Obudulu ◽  
Manoj K Srivastava ◽  
Robert Nilsson ◽  
...  
Keyword(s):  
Protein Expression ◽  
Tension Wood ◽  
Wood Formation

Analysis of Protein Expression along the Normal to Tension Wood Gradient in Eucalyptus gunnii

Holzforschung ◽  
2003 ◽  
Vol 57 (4) ◽  
pp. 353-358 ◽  
Author(s):  
C. Plomion ◽  
C. Pionneau ◽  
H. Baillères
Keyword(s):  
Protein Expression ◽  
Tension Wood ◽  
Lignin Content ◽  
Polyacrylamide Gel Electrophoresis ◽  
Wood Formation ◽  
Computer Assisted ◽  
Normal Wood ◽  
Growth Strain ◽  
Silver Nitrate Staining ◽  
Xylem Tissue

Abstract This paper examines the contribution of various xylem proteins to wood formation in Eucalyptus gunnii. Proteins were extracted from differentiating xylem harvested from a crooked tree, separated by high-resolution two-dimensional polyacrylamide gel electrophoresis, visualised by silver nitrate staining and analysed with a computer-assisted system for single protein spot quantification. Growth strain measurements allowed xylem samples to be classified quantitatively from tension wood to normal wood. Regression of lignin content on growth strain showed that a decrease in lignin content corresponded to decreasing growth strain values, i.e., presence of tension wood. Out of the 140 studied protein spots, 12 were significantly associated with growth strain: 7 being less abundant in tension wood and 5 being more abundant in tension wood. A clustered-correlation analysis was performed to study protein expression simultaneously along the gradient of gravistimulated stressed xylem tissue. Proteins were found to form “expression clusters”.

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