Characteristic Distance of Resonance Energy Transfer Coupled with Surface Plasmon Polaritons

2018 ◽  
Vol 9 (24) ◽  
pp. 7032-7039 ◽  
Author(s):  
Jhih-Sheng Wu ◽  
Yen-Cheng Lin ◽  
Yae-Lin Sheu ◽  
Liang-Yan Hsu
Keyword(s):  
Energy Transfer ◽  
Surface Plasmon ◽  
Surface Plasmon Polaritons ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Characteristic Distance ◽  
Plasmon Polaritons

scholarly journals Discovering Severe Acute Respiratory Syndrome Coronavirus 3CL Protease Inhibitors: Virtual Screening, Surface Plasmon Resonance, and Fluorescence Resonance Energy Transfer Assays

2006 ◽  
Vol 11 (8) ◽  
pp. 915-921 ◽  
Author(s):  
Lili Chen ◽  
Shuai Chen ◽  
Chunshan Gui ◽  
Jianhua Shen ◽  
Xu Shen ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Surface Plasmon Resonance ◽  
Virtual Screening ◽  
Severe Acute Respiratory Syndrome ◽  
Fluorescence Resonance Energy Transfer ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Resonance

An integrated system has been developed for discovering potent inhibitors of severe acute respiratory syndrome coronavirus 3C-like protease (SARS-CoV 3CLpro) by virtual screening correlating with surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) technologies-based assays. The authors screened 81,287 small molecular compounds against SPECS database by virtual screening; 256 compounds were subsequently selected for biological evaluation. Through SPR technology-based assay, 52 from these 256 compounds were discovered to show binding to SARS-CoV 3CLpro. The enzymatic inhibition activities of these 52 SARS-CoV 3CLpro binders were further applied to FRET-based assay, and IC50 values were determined. Based on this integrated assay platform, 8 new SARS-CoV 3CLpro inhibitors were discovered. The fact that the obtained IC50 values for the inhibitors are in good accordance with the discovered dissociation equilibrium constants (KDs) assayed by SPR implied the reliability of this platform. Our current work is hoped to supply a powerful approach in the discovery of potent SARS-CoV 3CLpro inhibitors, and the determined inhibitors could be used as possible lead compounds for further research.

Surface plasmon enhanced Förster resonance energy transfer between the CdTe quantum dots

2008 ◽  
Vol 93 (12) ◽  
pp. 123102 ◽  
Author(s):  
Vamsi K. Komarala ◽  
A. Louise Bradley ◽  
Yury P. Rakovich ◽  
Stephen J. Byrne ◽  
Yurii K. Gun’ko ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Surface Plasmon ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Cdte Quantum Dots ◽  
Förster Resonance Energy Transfer ◽  
Förster Resonance
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