High-Speed Chemical Imaging by Dense-Net Learning of Femtosecond Stimulated Raman Scattering

2020 ◽  
Vol 11 (20) ◽  
pp. 8573-8578
Author(s):  
Jing Zhang ◽  
Jian Zhao ◽  
Haonan Lin ◽  
Yuying Tan ◽  
Ji-Xin Cheng
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Stimulated Raman

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

scholarly journals Plasmon-enhanced stimulated Raman scattering microscopy with single-molecule detection sensitivity

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Cheng Zong ◽  
Ranjith Premasiri ◽  
Haonan Lin ◽  
Yimin Huang ◽  
Chi Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Detection Sensitivity ◽  
Single Molecule Detection ◽  
Label Free ◽  
Biomedical Systems ◽  
Stimulated Raman

AbstractStimulated Raman scattering (SRS) microscopy allows for high-speed label-free chemical imaging of biomedical systems. The imaging sensitivity of SRS microscopy is limited to ~10 mM for endogenous biomolecules. Electronic pre-resonant SRS allows detection of sub-micromolar chromophores. However, label-free SRS detection of single biomolecules having extremely small Raman cross-sections (~10−30 cm2 sr−1) remains unreachable. Here, we demonstrate plasmon-enhanced stimulated Raman scattering (PESRS) microscopy with single-molecule detection sensitivity. Incorporating pico-Joule laser excitation, background subtraction, and a denoising algorithm, we obtain robust single-pixel SRS spectra exhibiting single-molecule events, verified by using two isotopologues of adenine and further confirmed by digital blinking and bleaching in the temporal domain. To demonstrate the capability of PESRS for biological applications, we utilize PESRS to map adenine released from bacteria due to starvation stress. PESRS microscopy holds the promise for ultrasensitive detection and rapid mapping of molecular events in chemical and biomedical systems.

Effect of Fiber-Optics Nonlinearities in Long Haul and Ultra-High Speed DWDM Optical Transmission Networks at 10, 40 and 100 Gb/s Ultra-High Speed Data Rates

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Farman Ali ◽  
Yousaf Khan ◽  
Shahryar Shafique Qureshi ◽  
Shabir Ahmad ◽  
Muhammad Waqas
Keyword(s):  
Raman Scattering ◽  
Fiber Optics ◽  
Wavelength Division Multiplexing ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Ultra High Speed ◽  
Data Rates ◽  
High Speed Data ◽  
The Impact ◽  
Stimulated Raman

AbstractThe impact of major nonlinearities in optical fiber such as SRR (Stimulated Raman Scattering) and Four Wave Maxing (FWM) in cascaded amplifier Dense Wavelength Division Multiplexing optical long reach and high speed data rate transmission scheme have been investigated at different data rates. Pulse walk off effect is considered for the purpose of calculating Stimulated Raman Scattering (SRS) nonlinear factor. Examination has been accomplished to assess signal to noise ratio thinking about the consolidated impact of SRS and FWM within the sight of amplified spontaneous emission noise to achieve minutest noise at altered data rates.

Label-Free Chemical Imaging of Latent Fingerprints with Stimulated Raman Scattering Microscopy

2017 ◽  
Vol 89 (8) ◽  
pp. 4468-4473 ◽  
Author(s):  
Benjamin Figueroa ◽  
Yikai Chen ◽  
Kyla Berry ◽  
Andrew Francis ◽  
Dan Fu
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Latent Fingerprints ◽  
Stimulated Raman

scholarly journals Stimulated Raman Scattering Microscopy for Label-Free Chemical Imaging

2009 ◽  
Vol 20 (12) ◽  
pp. 30 ◽  
Author(s):  
Christian W. Freudiger ◽  
Wei Min ◽  
Brian G. Saar ◽  
X. Sunney Xie
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Stimulated Raman
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