Label-Free Imaging of Fibronectin Adsorption at Poly-(l-lysine)-Decorated Liquid Crystal Droplets

2019 ◽  
Vol 123 (22) ◽  
pp. 13642-13650 ◽  
Author(s):  
Indu Verma ◽  
Ipsita Pani ◽  
Diksha Sharma ◽  
Shiny Maity ◽  
Santanu Kumar Pal
Keyword(s):  
Liquid Crystal ◽  
Label Free

Label-free liquid crystal immunosensor for detection of HBD-2

Talanta ◽  
2019 ◽  
Vol 203 ◽  
pp. 203-209 ◽  
Author(s):  
Xiuxia Su ◽  
Wenjing Huo ◽  
Dong Yang ◽  
Chonglin Luan ◽  
Jia Xu
Keyword(s):  
Liquid Crystal ◽  
Label Free

scholarly journals Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2294
Author(s):  
Haw-Ming Huang ◽  
Er-Yuan Chuang ◽  
Fu-Lun Chen ◽  
Jia-De Lin ◽  
Yu-Cheng Hsiao
Keyword(s):  
Liquid Crystal ◽  
Smart Phone ◽  
Organic Polymer ◽  
The Novel ◽  
Label Free ◽  
Home Test ◽  
Sensing Technology ◽  
Highly Sensitive ◽  
Vertically Aligned ◽  
Alignment Layers

The highly sensitive interfacial effects between liquid crystal (LC) and alignment layers make LC-bioinspired sensors an important technology. However, LC-bioinspired sensors are limited by quantification requiring a polarized microscope and expensive equipment, which makes it difficult to commercialize LC-bioinspired sensors. In this report, we first demonstrate that dye-doped LC (DDLC) chips coated with vertically aligned layers can be employed as a new LC-bioinspired sensing technology. The DDLC-bioinspired sensor was tested by detecting bovine serum albumin (BSA) and immunocomplexes of BSA pairs. The intensities of the dye color of the DDLC-bioinspired sensor can be changed with the concentrations of biomolecules and immunocomplexes. A detection limit of 0.5 µg/mL was shown for the color-indicating DDLC-bioinspired sensors. We also designed a new method to use the quantitative DDLC-bioinspired sensor with a smart-phone for potential of home test. The novel DDLC-bioinspired sensor is cheap, label-free, and easy to use, furthering the technology for home and field-based disease-related detection.

scholarly journals A liquid-crystal-based DNA biosensor for pathogen detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mashooq Khan ◽  
Abdur Rahim Khan ◽  
Jae-Ho Shin ◽  
Soo-Young Park
Keyword(s):  
Liquid Crystal ◽  
Pathogen Detection ◽  
Grid Cell ◽  
High Specificity ◽  
Erwinia Carotovora ◽  
Optical Microscope ◽  
Label Free ◽  
Label Free Detection ◽  
Homeotropic Orientation ◽  
Tem Grid

Abstract A liquid-crystal (LC)-filled transmission electron microscopy (TEM) grid cell coated with the cationic surfactant dodecyltrimethylammonium bromide (DTAB), to which a single-stranded deoxyribonucleic acid probe (ssDNAprobe) was adsorbed at the LC/aqueous interface (TEMDTAB/DNA), was applied for the highly specific detection of target DNA molecules. The DTAB-coated E7 (used LC mixture) in the TEM grid (TEMDTAB) exhibited a homeotropic orientation, and changed to a planar orientation upon adsorption of the ssDNAprobe. The TEMDTAB/DNA was then exposed to complementary (target) ssDNA, which resulted in a planar-to-homeotropic configurational change of E7 that could be observed through a polarized optical microscope under crossed polarizers. The optimum adsorption density (2 μM) of ssDNAprobe enabled the detection of ≥0.05 nM complementary ssDNA. This TEMDTAB/DNA biosensor could differentiate complementary ssDNA from mismatched ssDNA as well as double-stranded DNA. It also successfully detected the genomic DNAs of the bacterium Erwinia carotovora and the fungi Rhazictonia solani. Owe to the high specificity, sensitivity, and label-free detection, this biosensor may broaden the applications of LC-based biosensors to pathogen detection.

Label-free optical detection of thrombin using a liquid crystal-based aptasensor

2018 ◽  
Vol 141 ◽  
pp. 71-79 ◽  
Author(s):  
Heesang Kim ◽  
Zongfu An ◽  
Chang-Hyun Jang
Keyword(s):  
Liquid Crystal ◽  
Optical Detection ◽  
Label Free

Label-free sensitive detection of MUC1 using a liquid crystal based-system

2020 ◽  
Vol 47 (12) ◽  
pp. 1784-1793 ◽  
Author(s):  
Thai Duong Song Duong ◽  
Chang-Hyun Jang
Keyword(s):  
Liquid Crystal ◽  
Sensitive Detection ◽  
Label Free

A novel liquid crystal sensing platform for highly selective UO22+ detection based on UO22+-specific DNAzyme

2021 ◽  
Author(s):  
Congcong Hu ◽  
Ping Li ◽  
Zhaoyang Wu ◽  
Pengfei Fan ◽  
Duo Qian ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Uranyl Ion ◽  
Label Free ◽  
Sensing Platform ◽  
Selective Sensor

A label-free and selective sensor was established for uranyl ion (UO22+) detection based on UO22+-dependent DNAzyme and liquid crystal (LC). In the presence of UO22+, the substrate chains can be...

Distinct interfacial ordering of liquid crystal observed by protein-lipid interactions that enabled the label free sensing of cytoplasmic protein at LC-aqueous interface

The Analyst ◽  
2021 ◽  
Author(s):  
Santanu Kumar Pal ◽  
Manisha Devi ◽  
Indu Verma
Keyword(s):  
Liquid Crystal ◽  
Protein Adsorption ◽  
Aqueous Phase ◽  
Protein Interactions ◽  
Cytoplasmic Protein ◽  
Label Free ◽  
Lipid Interactions ◽  
Lipid Protein Interactions

Interfaces formed between lipid decorated liquid crystal (LC) film and aqueous phase can mimic the bimolecular membrane where interfacial occurring biological phenonmenon (e.g., lipid-protein interactions, protein adsorption) can be visually...

scholarly journals Label-free protein sensing by employing blue phase liquid crystal

2017 ◽  
Vol 8 (3) ◽  
pp. 1712 ◽  
Author(s):  
Mon-Juan Lee ◽  
Chung-Huan Chang ◽  
Wei Lee
Keyword(s):  
Liquid Crystal ◽  
Label Free ◽  
Protein Sensing ◽  
Free Protein ◽  
Phase Liquid ◽  
Blue Phase
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